The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
권호 표지

Effects of Concurrent Administration of KKSDU and AJ on Atopic Dermatitis-like Skin Lesions in NC/Nga Mouse

아토피양(樣) 피부염 NC/Nga생쥐에서 가감소독음(加減消毒飮)과 아토피크림-자운고(紫雲膏)의 병용투여가 피부염에 미치는 영향

  • Song, Hyun-Jee (Department of Pediatrics, College of Oriental Medicine, Dae Jeon University) ;
  • Han, Jae-Kyung (Department of Pediatrics, College of Oriental Medicine, Dae Jeon University) ;
  • Kim, Yun-Hee (Department of Pediatrics, College of Oriental Medicine, Dae Jeon University)
  • 송현지 (대전대학교 한의과대학 소아과학교실) ;
  • 한재경 (대전대학교 한의과대학 소아과학교실) ;
  • 김윤희 (대전대학교 한의과대학 소아과학교실)
  • Published : 2009.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : The purpose of this study is to investigate the effect of concurrent administration of KKSDU and AJ on atopic dermatitis in an in-vivo experiment using an NC/Nga atopic dermatitis mouse, which has histological and clinical similarities to the condition in humans. Methods : We evaluated clinical skin score, hematology, serum total IgE and IgG1 of NC/Nga atopic dermatitis mouse and analyzed the cytoline level, total cell number, immunohistochemical staining, histological features of axillary lymph node(ALN), draining lymph node(DLN), peripheral blood mononuclear cells(PBMCs) and dorsal skin tissue in NC/Nga mouse. Results : Orally administration of KKSDU and concurrent administration of KKSDU and AJ decreased the clinical skin score, total cell number of WBC, platelet, neutrophils, eosinophils in blood, serum total IgE & IgG1, IL-5, IL-13. Also, total cell number of ALN and dorsal skin tissue, absolute cell number of CD3e+&CD19+, CD4+&CD8+, CD3+/CCR3+, CCR3+, CD3+/CD69+, CD3+/CXCR5+ in ALN, PBMCs, absolute cell number of CCR3+, CD3+/CD69+, CD11b+/Gr-1+ in dorsal skin tissue, Eotaxin2 mRNA, CCR3 mRNA in dorsal skin tissue and gene expression of IL-5 mRNA, IL-13 mRNA in ALN are significantly decreased. Furthermore, thickness of epidermis, infiltrated inflammatory immune cell & mast cell in dermis, histologic infiltration of mast cell, the size of inflammatory lymphocytes cells & plasma cells in ALN and histologic infiltration of CD4+ & CCR3+ in ALN and dorsal skin tissue are significantly decreased. However, total cell number of DLN, absolute cell number of CD3+&CD19+, CD4+&CD8+, B220+/CD23+, CD3+/CD69+ in DLN and CD4+CD25+foxp3+Treg cell, foxp3 mRNA in dersal skin tissue are increased significantly. Conclusions : Concurrent administration of KKSDU and AJ on atopic dermatitis in an in-vivo experiment using an NC/Nga atopic dermatitis mouse was very effective to the atopic detmatitis treatment.

Keywords

References

  1. Finlay AY. Quality of life in atopic dermatitis. J Am Acad Dermatol. 2001;45:S64-6. https://doi.org/10.1067/mjd.2001.117010
  2. Lewis-Jones S. Quality of life and childhood atopic dermatitis: the misery of living with childhood eczema. Int J Clin Pract. 2006;60:984-92. https://doi.org/10.1111/j.1742-1241.2006.01047.x
  3. Feldman S, Behnam SM, Behnam SE, Koo JYM. Involving the patient: impact of inflammatory skin disease and patient-focused care. J Am Acad Dermatol. 2005;53:S78-85. https://doi.org/10.1016/j.jaad.2005.04.033
  4. 안효섭. 홍창의. 소아과학. 서울:대한교과서주식회사. 2004:1166-92, 1207-11.
  5. 허인희, 홍성민, 엄유식, 변학성, 심성용, 김경준. 외치요법을 위주로 한 아토피 피부염환자 증례. 한방안이비인후과피부과학회지. 2006;9(3):201-8.
  6. 진경선, 임태형, 박은정, 이해자, 한경훈, 나원경. 淸上防風湯의 아토피성 알레르기 반응 조절 효과. 대한한방소아과학회지. 2004;18(2):61-76.
  7. 유한정, 이진용, 김덕곤. 內消和中湯加味方이 아토피 피부염 환아의 혈중 Cytokine 분비에 미치는 영향. 대한한방소아과학회지. 2006;20(1):15-29.
  8. 김홍배, 김원일, 이승연. 升麻葛根湯의 항히스타민 효과 및 IL-4와 GM-CSF 발현에 미치는 영향. 대한한방소아과학회지. 2006;20(2):69-83.
  9. 박성의, 진미림, 구영선, 김동희. 脾虛蘊濕方이 아토피 피부염 유발 NC/Nga 생쥐의 혈액 내 면역관련인자에 미치는 영향. 동의생리병리학회지. 2007;21(4):849-55.
  10. 손정민, 홍승욱. 防風通聖散이 아토피 피부염을 유발한 동물모델의 피부 손상에 미치는 영향. 한방안이비인후피부과학회지. 2007;20(1):99-114.
  11. 신상호, 김미보, 변석미, 이호섭, 박선규, 고우신, 윤화정. 아토피 피부염의 외치법에 대한 고찰. 한방안이비인후피부과학회지. 2008;21(2):80-93.
  12. 황치환, 윤채성, 송승현, 원영호, 황충연. 葛根解肌湯加味方과 三黃洗劑加味方 병용이 NC/Nga 생쥐의 아토피 피부염에 미치는 영향. 한방안이비인후피부과학회지. 2008;21(2):1-18.
  13. 민영규, 최정화, 김종한, 박수연. 消風散加減方과 外治方 병용이 NC-Nga 아토피 생쥐에 미치는 영향. 대한이비인후피부과학회지. 2005;18(1):116-34.
  14. 김정진, 양성완, 손낙원, 안규석. 加味生料四物湯의 항염증 효과와 止痒膏의 아토피피부염 손상 및 止痒 효과에 미치는 영향. 동의생리병리학회지. 2003;17(2):428-35.
  15. 홍철희, 서은성, 원영호, 김영근, 황충연. 溫淸飮과 三黃洗劑加味方 병용이 NC/Nga 아토피 생쥐에 미치는 영향. 동의생리병리학회지. 2007;21(3):679-87.
  16. 金永勳. 晴崗醫鑑. 서울:성보사. 1984:361.
  17. 韓醫科大學 方劑學敎授 共編著. 方劑學. 서울:영림사. 1999:92-3.
  18. 송현지, 김윤희, 한재경. 아토피樣 피부염 NC/Nga생쥐에서 加減消毒飮의 투여가 피부염에 미치는 영향. 대한한방소아과학회지. 2009;23(1):23-35.
  19. 大田大學校 韓方病院. 韓方病院 處方集. 大田:韓國出版社. 2001:542.
  20. 蔡炳允. 韓方外科. 서울:고문사. 1991:397.
  21. 조태동, 송진희. 허브 아로마 라이프. 서울:대원사. 2006:19-23,29,41,43.
  22. 문수진. 에센셜 오일의 여드름피부 개선효과-lavender, tea tree, 캐모마일 오일의 피부 임상중심. 중앙대학교 의약식품대학원 석사학위논문. 2005.
  23. 조성준. 향기치료의 기적. 서울:도서출판宇石. 2000:120,140,169-71.
  24. Dogru M., Katakami C., Nakagawa N., Tetsumoto K., and Yamamoto M. Impression cytology in atopic dermatitis. Ophthalmology. 1998;105:1478-84. https://doi.org/10.1016/S0161-6420(98)98032-0
  25. Hoffler U., Oette K. Comparative studies on four thrombocyte counting methods: counting in the smear preparation after Fonio, chamber counting in the phase contrast microscope, counting with the Coulter Thrombocounter, and the Technicon Auto-Counter. Z Klin Chem Klin Biochem. 1974;12(5):198-206.
  26. Matsuda H., N. Watanabe, G.P. Geba, J. Sperl, M. Tsudzuki, J. Hiroi. Development of atopic dermatitis like skin lesion with IgE hyperproduction in NC/Nga mouse. Int Immunol. 1997;9:461-6. https://doi.org/10.1093/intimm/9.3.461
  27. Galli SJ. Allergy. Curr. Biol. 2006;10:R93-5.
  28. Yuki Hashimoto, Iwao Arai, Yutaka Nakanishi, Takanobu Sakurai, Atsushi Nakamura. Scratching of their skin by NC/Nga mouse leads to development of dermatitis. Life Sciences. Japan 17 March. 2004;76:783-94. https://doi.org/10.1016/j.lfs.2004.07.022
  29. K Johrens, I Anagnostopoulos, H Durkop & H Stein. Different T-bet expression patteerns characterize particular reactive lymphoid tissue lesions. Histopathology. 2006;48:343-52. https://doi.org/10.1111/j.1365-2559.2005.02305.x
  30. Danniel W. A foundation for analysis in the health science. Biostics. 1983:136-46.
  31. 안성구, 박병덕, 원진형, 성열오, 천승현. 개원의를 위한 아토피 피부염. 서울:퍼시픽출판사. 2007:25,35.
  32. 김정희. 아토피 피부염의 최신 지견. 소아알레르기 및 호흡기학회지. 2004;14(1):12-23.
  33. Leung DYM. Pathogenesis of atopic dermatitis. J Allergy Clin Immunol. 1999;104:99-108. https://doi.org/10.1016/S0091-6749(99)70150-8
  34. 이지현, 김규한, 김명남, 김진우, 노영석, 박영립, 박천욱, 이광훈, 이애영, 조상현, 최지호. 한국 아토피피부염의 치료지침. 대한피부과학회지. 2006;44(8):907-13.
  35. 이상규. Immune System-Two Face of the Same Coin. 분자세포생물학뉴스. 2008;20(1):70-7.
  36. Nakamura K, Kitani A, Strober W. Cell contact-dependent immuno-suppression by CD4+CD25+ regulatory T cells is mediated by cell surFSCe-bound transforming growth factor beta. J Exp Med. 2001;194:629-44. https://doi.org/10.1084/jem.194.5.629
  37. Brunkow ME, Jeffery EW, Hjerrild KA, Paeper B, Clark LB, Yasayko SA, Wilkinson JE, Galas D, Ziegler SF, Ramsdell F. Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse. Nature genetics. 2001;27(1):68-73.
  38. Tone Y, Furuuchi K, Kojima Y, Tykocinski ML, Greene MI, Tone M. Smad3 and NFAT cooperate to induce foxp3 expression through its enhancer. Nature Immunology. 2008;9(2):194-202. https://doi.org/10.1038/ni1549
  39. 錢乙. 小兒藥證直訣. 북경:인민위생출판사. 1985:212-6.
  40. 윤길영. 동의방제학. 서울:고문사, 1980:48-51, 64.
  41. 전국한의과대학 본초학교수 공편저. 본초학. 서울:영림사. 2000:196-7, 578-80.
  42. 陳實功. 外科正宗. 上海:上海科學技術出版社. 1989:269, 303.
  43. 矢數道明. 漢方治療百話(6권). 서울:東南出版社. 1991:322.
  44. 吳謙. 醫宗金鑑. 서울:大星文化社. 1983:80.
  45. 祁坤. 外科大成. 台北:文光圖書有限公司. 1975:212
  46. 邱嗣康. 中醫外科. 北京:人民衛生出版社. 1989:233.
  47. 김창민, 신민규, 안덕균, 이경순. 중약대사전. 서울:도서출판 정담. 1997:887-95, 3611-8, 1182-3, 1458-60.
  48. 임종국, 안준철, 문진영. 當歸藥針液의 항산화효능에 관한 연구(2). 대한침구학회지. 1997;14(1):383-96.
  49. 오하식, 김형환, 안덕균, 최호영. 當歸類가 혈관신생에 미치는 영향에 대한 비교연구. 대한본초학회지. 2001;16(2):19-26.
  50. 김시혜, 정혁상, 조백건, 이진용, 김덕곤. 紫草가 아토피 피부염에 미치는 영향. 대한한방소아과학회지. 2004;18(1):63-75.
  51. 박대원, 이용섭, 서영배, 안덕균, 최호영. 생쥐의 B 세포에서 anti-CD40과 $\gamma$IL-4로 유도된 cytokine 생산에 대한 紫草의 효과. 대한본초학회지. 2004;16(2):19-27.
  52. 남상윤, 이범준. 랫드의 화상모델에서 전복껍질과 참기름의 치료효과. 한국수의공중보건학회지. 2003;27(2):119-27.
  53. 김수경, 최해윤, 지선영, 이상곤. 紫雲膏와 Gentamicin 倂用이 創傷治癒 및 創傷感染에 미치는 影響에 關한 硏究. 대한본초학회지. 2004;19(4):137-60.
  54. 전재홍. 紫雲膏가 자외선에 의한 피부손상과 광노화에 미치는 영향. 동국대학교 박사학위논문. 2006.
  55. 공태영, 박태열. 紫雲膏(A)를 이용한 火傷 치료 및 피부질환치험례. 한의외치요법학회지. 2000;2(1):53-7.
  56. 조소은. 병리학적 관점에서의 아토피 피부염의 치료와 스킨케어 방향에 관한 연구. 공주영상정보대학 논문집. 2001;8:637-51.
  57. Schreiber SL and Crabtree GR. The mechanism of action of cyclosporin A and FK506. Immunol Today. 1992;13(4):136-42. https://doi.org/10.1016/0167-5699(92)90111-J
  58. H Matsuda, N Watanabe, GP Geba, J Sperl, M Tsudzuki, J Hiroi, M Matsumoto, H Ushio, S Saito, PW Askenase and C Ra. Development of atopic dermatitis-like skin lesion with IgE hyperproduction in NC/Nga mouse. International Immunology. 1997;9:461-6. https://doi.org/10.1093/intimm/9.3.461
  59. Tocci MJ, Matkovich DA, Collier KA. The immunosupressant FK506 selectively inhibits expression of early T cell activation genes. J Immunol. 1989;143:718-26.
  60. Jun Hiroi, Takanori Sengoku, Kyoko Morita, Shinichi Kishi, Sachio Sato, Toshikazu Ogawa, Masaoki Tsudzuki, Hiroshi Matsuda, Adumi Wada and Kozaburo Esaki. Effect of Tacrolimus Hydrate(FK506) Ointment on Spontaneous Dermatitis in NC/Nga Mice. The Japanese Journal of Pharmacology. 1998;76:175-83. https://doi.org/10.1254/jjp.76.175
  61. Elena Gonzalez-Rey, Alejo Chorny and Mario Delgado. Regulation of immune tolerance by anti-inflammatory neuropeptides. Nat Rev Immunol. 2007;7:52-63. https://doi.org/10.1038/nri1984
  62. Leung DY. Atopic dermatitis: new insights and opportunities for therapeutic tervention. J Allergy Clin Immunol. 2000;105(5):860-76. https://doi.org/10.1067/mai.2000.106484
  63. Christian Vestergaard, Hiroyuki Yoneyama, Masako Murai, Kohichiro Nakamura, Kunihiko Tamaki, Yuya Terashima, Toshio Imai, Osamu Yoshie, Tatsuro Irimura, Hitoshi Mizutani, and Kouji Matsushima1. Overproduction of Th2-specific chemokines in NC/Nga mouse exhibiting atopic dermatitis–like lesions. J Clin Invest. 1999;104(8):1097–105.
  64. Ryouji Yagi, Haruko Nagai, Yutaka Iigo, Toshihiro Akimoto, Takao Arai and Masato Kubo. Development of Atopic Dermatitis-Like Skin Lesions in STAT6-Deficient NC/Nga mouse. The Journal of Immunology. 2002;168:2020-7. https://doi.org/10.4049/jimmunol.168.4.2020
  65. Tatsuya Sasakawaa, Yasuyuki Higashib, Syozo Sakumac, Yoshitaka Hirayamaa, Yuka Sasakawaa, Yoshitaka Ohkuboa, Toshio Gotoa, Masahiro Matsumotod, Hiroshi Matsudae. Atopic Dermatitis-Like Skin Lesions Induced by Topical Application of Mite Antigens in NC/Nga mouse. Int Arch Allergy Immunol. 2001;126:239-47. https://doi.org/10.1159/000049520
  66. Kim CH, Rott LS, Clark-Lewis I, Campbell DJ, Wu L, Butcher EC. Subspecialization of CXCR5+ T cells : B helper activity is focused in a germinal center-localized subset of CXCR5+ T cells. J Exp Med. 2001;18;193(12):1373-81. https://doi.org/10.1084/jem.193.12.1373
  67. Masayuki Heishi, Yukiho Imai, Hiroko Katayama, Ryoichi Hashida, Mikito Ito, Akira Shinagawa, Yuji Sugita. Gene Expression Analysis of Atopic Dermatitis-Like Skin Lesions Induced in NC/Nga mouse by Mite Antigen Stimulation under Specific Pathogen-Free Conditions. Int Arch Allergy Immunol. 2003;132:355-63. https://doi.org/10.1159/000074903
  68. Bo Huang, Ping-Ying Pan, Qingsheng Li, Alice I. Sato, David E. Levy, Jonathan romberg, Celia M. Divino and Shu-Hsia Chen. Gr-1+CD115+ Immature Myeloid Suppressor Cells Mediate the Development of Tumor-Induced T Regulatory Cells and T-Cell Anergy in Tumor-Bearing Host. Cancer Research. 2006;15:1123-31.
  69. Ebtekar M. Effects of Persistent Organic Pollutants on the Immune System: The Case of Dioxins. Iranian Journal of Environmental Health Science & Engineering. 2004;2:1-7.
  70. Wolfgang Weninger, and Ulrich H. von Andrian. Chemokine regulation of naïve T cell traffic in health and disease. Seminars in Immunology. 2003;15:257-70.