Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Anti-Obesitic Effects of Cathepsin S Inhibitory Fraction Derived from Paecilomyces tenuipes in Mice Fed a High-Fat Diet

고지방식이를 섭취한 쥐에서 눈꽃동충하초 유래 cathepsin S 저해 분획의 비만 억제 효과

  • 허건 ((주)한국야쿠르트 중앙연구소) ;
  • 명길선 ((주)한국야쿠르트 중앙연구소) ;
  • 이정희 ((주)한국야쿠르트 중앙연구소) ;
  • 허철성 ((주)한국야쿠르트 중앙연구소)
  • Published : 2009.08.31
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Abstract

In this study, cathepsin S (CTSS) inhibitory fraction was isolated from Paecilomyces tenuipes and anti-obesitic effects of the fraction were evaluated in mice, fed a high-fat diet. Hot water extract of P. tenuipes (DHW) was divided into 2 fractions, water eluate fraction (DHP1) and methanol eluate fraction (DHP2) using Diaion HP-20. $IC_{50}$ values for DHW, DHP1 and DHP2 against CTSS were 108.7, 890.3 and 2.3 ${\mu}g$/mL, respectively. To evaluate anti-obesitic effects of the fractions, each fraction was administrated orally to C57BL/6 mice for 4 weeks with a high-fat diet. DHP2 had the highest inhibitory effect on CTSS activity, causing serious reduction in weight gain, a reduction in the amount of adipose tissue and in serum lipids levels. These results suggest that the inhibition of CTSS by compounds derived from P. tenuipes may be effective in preventing and in ameliorating obesity.

Cysteine protease의 일종인 CTSS가 비만에 관여한다는 것이 알려진 후 CTSS저해를 통한 비만 억제의 가능성이 제시되고 있다. 본 연구는 눈꽃동충하초로부터 CTSS저해 활성을 갖는 분획을 분리하여 항비만 효과를 확인하는 것을 목적으로 수행되었다. 눈꽃 동충하초 열수추출물을 Diaion HP-20 수지 흡착 여부에 따라서 DHP1, DHP2 두 개의 분획으로 나누었다. CTSS에 대한 $IC_{50}$값은 열수추출물, DHP1, DHP2가 각각 108.7, 890.3, 2.3 ${\mu}g$/mL이었다. 고지방식이로 비만을 유도한 마우스에 각 분획들을 4주간 섭취시키고 체중 변화, 부고환 지방 무게, 지방세포 크기 및 혈중지질 농도 등을 측정한 결과 CTSS 저해활성이 가장 높은 분획 DHP2를 섭취시킨 실험군에서 가장 뛰어난 항비만 효능을 확인할 수 있었다. 결론적으로 본 연구는 눈꽃동충하초로부터 분리된 CTSS저해 성분들이 비만을 치료하거나 예방하는데 효과적으로 활용될 수 있는 가능성을 제시한다고 볼 수 있다. 향후 눈꽃동충하초로부터 CTSS저해 물질을 분리 및 동정한다면 보다 명확한 항비만 기작을 밝혀낼 수 있을 것이다.

Keywords

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