Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Acetylcholinesterase Inhibitory Effect of Green Tea Extracts According to Storage Condition

저장 조건에 따른 녹차 추출물의 acetylcholinesterase(AChE) 저해 효과

  • Kwak, Ji Hyun (Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jeong, Chang-Ho (Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Ji Hye (Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Choi, Gwi Nam (Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Shin, Young-Hee (Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Seung-Cheol (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Cho, Sung Hwan (Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Choi, Sung-Gil (Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Heo, Ho Jin (Division of Applied Life Sciences, Institute of Agriculture and Life Science, Gyeongsang National University)
  • 곽지현 (경상대학교 응용생명과학부.농업생명과학연구원) ;
  • 정창호 (경상대학교 응용생명과학부.농업생명과학연구원) ;
  • 김지혜 (경상대학교 응용생명과학부.농업생명과학연구원) ;
  • 최귀남 (경상대학교 응용생명과학부.농업생명과학연구원) ;
  • 신영희 (경상대학교 응용생명과학부.농업생명과학연구원) ;
  • 이승철 (경남대학교 식품생명학과) ;
  • 조성환 (경상대학교 응용생명과학부.농업생명과학연구원) ;
  • 최성길 (경상대학교 응용생명과학부.농업생명과학연구원) ;
  • 허호진 (경상대학교 응용생명과학부.농업생명과학연구원)
  • Published : 2009.08.31
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Abstract

Acetylcholinesterase (AChE) inhibitors, which enhance cholinergic transmission by reducing the enzymatic degradation of acetylcholine, are the only source of the compound that is currently approved for the treatment of Alzheimer's disease (AD). In these experiments, the concentration samples of green tea extracts were 100, 500, and 1,000 ${\mu}g$/mL. Among them, the highest concentration (1,000 ${\mu}g$/mL) of fresh green tea extracts showed the most potent inhibitory effect on AChE by reducing more than 40% of enzyme activity, and in a concentration-dependent pattern. In addition, we examined the effect of other storing conditions on AChE inhibition. In order to maintain storage for 3 months, the materials were held at the certain storing conditions of temperature (room temperature, 4 and $-20^{\circ}C$) and for water activity (0.81, 0.69, and 0.23). In these storing conditions, the difference in temperature did not contribute to the AChE inhibitory effect. Our presented data showed that the AChE inhibitory effect was affected by the concentration of green tea extract and by water activity (0.81). These results suggest that green tea may serve as a potential dietary source of AChE inhibitor.

잎녹차 추출물 보다는 분말녹차 추출물의 경우가 그리고 저장된 sample보다는 fresh sample의 AChE 저해활성이 상대적으로 높았고 이는 생리활성 물질의 추출효율성과 생리활성 물질의 특성 변화 측면에서 기인되는 것으로 판단된다. 또한 본 연구팀에서 구성한 저장 조건에서, 4주간의 짧은 저장기간이 3개월 저장조건보다는 우수한 효소 저해활성도를 나타냈다. 수분활성도 측면에서는 수분활성도 0.81의 조건을 갖는 sample에서의 효과가 비교적 우수했고, 3개월 저장하는 동안의 저장온도 조건은 효소 저해 활성도에 큰 차이를 보이는 요소로 작용하지 못했다. 다만 상온이나 냉장조건 보다는 냉동상태의 저장조건이 다소 우수한 것으로 판단된다. 결국 생리활성물질을 보다 효율적으로 추출할 수 있는 녹차의 상태와 처리 또는 섭취 농도가 더욱 중요한 것으로 사료되고, 추출 효율을 증가시키기 위해서는 잎 보다는 분말의 상태가 더 우수한 것으로 판단된다. 따라서 녹차 추출물은 acetylcholine성 신경세포의 기능을 강화시켜줄 수 있는 AChE 저해제로서 활용가치가 높을 것이다.

Keywords

References

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