Polyphenol and Anti-oxidant Effects of Kalopanax septemlobus Koidz. Leaf Extracts

음나무(Kalopanax septemlobus Koidz.) 잎 추출물의 폴리페놀 및 항산화효과

  • Jun, Dong-Ha (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Lee, Jin-Tae (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Cheon, Soon-Ju (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Lee, Chang-Eon (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Kim, Tae-Hoon (Department of Herbal Medicinal Pharmacology, Daegu Haany University) ;
  • Lee, Do-Hyung (Department of Forest Resources, Yeungnam University) ;
  • Han, Jin-Gyu (Korea Forest Research Institute) ;
  • Kim, Sea-Hyun (Korea Forest Research Institute)
  • 전동하 (대구한의대학교 화장품약리학과) ;
  • 이진태 (대구한의대학교 화장품약리학과) ;
  • 천순주 (대구한의대학교 화장품약리학과) ;
  • 이창언 (대구한의대학교 화장품약리학과) ;
  • 김태훈 (대구한의대학교 한약재약리학과) ;
  • 이도형 (영남대학교 산림자원학과) ;
  • 한진규 (국립산림과학원) ;
  • 김세현 (국립산림과학원)
  • Published : 2009.08.30

Abstract

The aim of the study was to assess the cosmeceutical activity of Kalopanax septemlobus leaf and it is possible that can be used as a cosmetic ingredient for applicaiton of cosmetic industries. The concentration of total phenolic compound of hot water and 70% EtOH extracts of K. septemlobus leaf showed 104 mg/L and 125 mg/L respectively. In the result of DPPH(1,1- diphenyl-2-picryl -hydrazyl) scavenging radical activity, 70% EtOH extracts of K. septemlobus leaf showed 93.1% and it was similar to BHA(butylated hydroxyanisole) effect at 1,000ppm concentration. Xanthine oxidase inhibition of hot water extracts and 70% EtOH extracts of K. septemlobus leaf were 46.6% and 60.4% at 1,000ppm, respectively. In these results, K. septemlobus leaf has a great potential as a cosmeceutical ingredient with a natural anti-oxidant source.

음나무 잎 추출물의 생리활성을 분석하기 위해 열수추출물과 70% EtOH 추출물의 폴리페놀의 함량을 측정하였으며, 항산화활성 분석을 위해 전자공여능, nitric oxide radical 저해활성, xanthine oxidase 저해활성, ABTS radical cation decolorization을 측정하였다. 폴리페놀의 총 함량은 열수추출물과 70% EtOH 추출물에서 각각 104 mg/L, 125 mg/L으로 정량되어 70% EtOH 추출물이 열수추출 물에 비해 높은 함량을 보였다. DPPH radical 소거능은 70% EtOH 추출물이 1,000 ppm에서 93.1%로 나타나 같은 농도의 BHA와 유사한 소거능을 보였다. nitric oxide radical 저해활성은 70% EtOH 추출물과 열수추출물이 1,000 ppm에서 49.6%, 49.5%로 나타나 같은 농도의 BHA보다 높은 nitrit oxide radical 저해활성을 보였다. Xanthine oxidase 저해활성은 1,000 ppm농도에서 열수 추출물과 70% EtOH 추출물에서 각각 46.6%, 60.4% 활성을 나타내었다. ABTS radical cation decolorization 활성은 1,000 ppm에서 열수추출물이 92.7%, 70% EtOH 추출물이 91.4%로 나타났다. 이상의 결과로 보아 음나무 잎의 70% EtOH 추출물과 열수추출물 모두 많은 폴리페놀이 함유되어 있으며, 70% EtOH 추출물과 열수추출물 모두 합성항산화제에 비해 항산화 활성이 다소 낮지만 천연 항 산화제로 우수한 항산화활성을 나타내는 것으로 분석되었다. 이를 바탕으로 음나무 잎의 강한 항산화능을 피부노화 예방관련 미용식품 및 기능성 화장품 소재로 활용 가능한 유용한 수종으로 개발 될 수 있을 것이다.

Keywords

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