Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of Treatment with Docosahexaenoic Acid into N-3 Fatty Acid Adequate Diet on Learning Related Brain Function in Rat

N-3계 지방산 적절 함량 식이의 docosahexaenoic acid 첨가가 기억력 관련 뇌 기능에 미치는 영향

  • Lim, Sun-Young (Division of Marine Environment & Bioscience, Korea Maritime University)
  • 임선영 (한국해양대학교 해양환경생명과학부)
  • Published : 2009.07.30
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Abstract

The effect of adding docosahexaenoic acid into an n-3 fatty acid adequate diet on the improvement of learning related brain function was investigated. On the second day after conception, Sprague Dawley strain dams were subjected to a diet containing either n-3 fatty acid adequate (Adq, 3.4% linolenic acid) or n-3 fatty acid adequate+docosahexaenoic acid (Adq+DHA, 3.31%linolenic acid plus 9.65% DHA). After weaning, male pups were fed on the same diet of their respective dams until adulthood. Motor activity and Morris water maze tests were measured at 10 weeks. In the motor activity test, there were no statistically significant differences in moving time and moving distance between the Adq and Adq+DHA diet groups. The n-3 fatty acid adequate with DHA (Adq+DHA) group tended to show a shorter escape latency, swimming time and swimming distance compared to the n-3 fatty acid adequate group (Adq), but the differences were not statistically significant. There was no difference in resting time, but the Adq+DHA group showed a higher swimming speed compared to the Adq group. In memory retention trials, the numbers of crossing of the platform position (region A), in which the hidden platform was placed, were significantly greater than those of other regions for both Adq and Adq+DHA groups. Based on these results, adding DHA into the n-3 fatty acid adequate diet from gestation to adulthood tended to induce better spatial learning performance in Sprague Dawley rats as assessed by the Morris water maze test, although the difference was not significant.

SD계 흰쥐를 사용하여 motor activity 실험에서 동물들의 자발적인 운동성을 측정한 결과, 주어진 시간 내 움직인 시간과 움직인 거리에는 n-3 지방산이 적절히 함유된 식이군 (Adq group)과 DHA가 첨가된 식이군(Adq+DHA group)간에 유의적 차이를 관찰할 수가 없었다. 학습효과 실험에서 n-3 지방산이 적절히 함유된 식이군 (Adq group)의 경우 목적 플랫트폼까지 걸리는 시간이 DHA 첨가 식이군(Adq+DHA group)에 비하여 다소 길었으나 유의적 차이는 관찰할 수가 없었다. 수영 속도(swimming speed)에서 DHA 첨가 식이군(Adq+DHA group)의 경우, n-3 지방산이 적절히 함유된 식이군 (Adq group)에 비해 유의적으로 빨랐으나 수영 풀에서 움직인 거리 (swimming distance)에는 두 식이군 간의 유의적 차이가 없었다. 두 식이군의 흰쥐들이 수영한 시간(swimming time)과 쉬고 있는 시간(resting time)의 경우, 쉬는 시간에는 유의적 차이가 없었으나 수영 시간 또한 n-3 지방산이 적절이 함유된 식이(Adq group)로 사육된 쥐가 DHA가 첨가된 식이로 사육된 쥐(Adq+DHA group)보다 수영한 시간이 길었으나 여기서도 유의적 차이는 없었다. 기억력 테스트에서 n-3 지방산이 적절히 함유된 식이군(Adq group) 및 DHA가 첨가된 식이군(Adq+DHA group) 모두는 목적 플랫트폼이 있었던 A 지역에 대한 기억이 우수하여 다른 지역들인 B, C, D를 지나가는 횟수보다 유의적으로 많았음을 관찰하였다(p<0.05). 이상의 결과로부터 임신에서부터 성인이 될 때까지 n-3 지방산이 적절히 함유된 식이로 사육된 쥐와 비교할 때 DHA가 첨가된 식이로 사육된 흰쥐가 Morris water maze를 이용한 공간기억력 실험에서 다소 우수한 기억 학습효과를 나타내었으나 유의적 차이는 없었음을 관찰 할 수가 있었다.

Keywords

References

  1. Carrie, I., P. Guesnet, J. M. Bourr, and H. Frances. 2000. Diets containing long-chain n-3 polyubsaturated fatty acids affect behavior differently during development than aging in mice. Br. J. Nutr. 83, 439-447
  2. Connor, W. E. and M. Neuringer. 1988. The effect of n-3 fatty acid deficiency and repletion upon the fatty acid composition and function of brain and retina. pp. 275-294, In Karnovsky, M. L., A. Leaf, and L. C. Bolls (eds.), Biological Membranes: Aberrations in Membrane Structure and Function, Liss Inc., New York
  3. Gamoh, S., M. Hahimoto, K. Sugioka, M. Shahdat-Hossain, Y. Misawa and S. Masumura. 1991. Chronic administration of docosahexaenoic acid improves reference memoryrelated learning ability in young rats. Neurosci. 93, 237-241
  4. Green, P. and E. Yavin. 1998. Mechanisms of docosahexaenoic acid accretion in the fetal brain. J. Neurosci. Res. 52, 129-136 https://doi.org/10.1002/(SICI)1097-4547(19980415)52:2<129::AID-JNR1>3.0.CO;2-C
  5. Greiner, R. S., T. Moriguchi, B. M. Slotnick, A. Hurron, and N. Salem. 2001. Olfactory discrimination deficits in n-3 fatty acid-deficient rats. Physiol. Behav. 72, 379-385 https://doi.org/10.1016/S0031-9384(00)00437-6
  6. Lim, S. Y. and H. Suzuki. 2000. Intakes of dietary docosahexaenoic acid ethyl ester and egg phosphatidylcholine improve maze-learning ability in young and old mice. J. Nutr. 130, 1629-1632
  7. Lim, S. Y. and H. Suzuki. 2001. Changes in maze behavior of mice occur after sufficient accumulation of docosahexaenoic acid in brain. J. Nutr. 131, 319-324
  8. Lim, S. Y. and H. Suzuki. 2005. Effect of dietary docosahexaenoic acid on maze-learning ability in aged mice fed n-3 fatty acid deficient diet. Food Sci. Biotechnol. 14, 788-792
  9. Lim, S. Y. 2007. Effect of supplementation with docosahexaenoic acid from gestation to adulthood on spatial learning performance in rat. J. Life Sci. 17, 1400-1405 https://doi.org/10.5352/JLS.2007.17.10.1400
  10. Moriguchi, T., R. Greiner, and N. Salem. 2000. Behavioral deficits associated with dietary induction of decreased brain docosahexaenoic acid concentration. J. Neurochem. 75, 2563-2573 https://doi.org/10.1046/j.1471-4159.2000.0752563.x
  11. Moriguchi, T. and N. Salem. 2003. Recovery of brain docosahexaenoate leads to recovery of spatial task performance. J. Neurochem. 87, 297-309 https://doi.org/10.1046/j.1471-4159.2003.01966.x
  12. Morris, R. 1984. Developments of a water-maze procedure for studying spatial learning in the rat. J. Neurosci. Methods 11, 47-60. https://doi.org/10.1016/0165-0270(84)90007-4
  13. Niu, S. L., D. C. Mitchell, S. Y. Lim, Z. M. Wen, H. Y. Kim, N. Salem, and B. J. Litman. 2004. Reduced G proteincoupled signaling efficiency in retinal rod outer segments in response to n-3 fatty acid deficiency. J. Biol. Chem. 279, 31098-31104 https://doi.org/10.1074/jbc.M404376200
  14. Reeves, P. G., F. H. Neilsen, and G. C. Fahey. 1993. Committee report on the AIN-93 purified rodent diet. J. Nutr. 123, 1939-1951
  15. Salem, N., B. Litman, H. Y. Kim, and K. Gawrisch. 2001. Mechanisms of action of docosahexaenoic acid in the nervous system. Lipids 36, 945-959 https://doi.org/10.1007/s11745-001-0805-6
  16. SanGiovanni, J. P., S. Parra-Cabrera, G. A. golditz, C. S. Berkey, and J. T. Dwyer. 2000. Meta-analysis of dietary essential fatty acids and long-chain polyunsaturated fatty acids as they related to visual resolution acuity in healthy preterm infants. Pediatr. 105, 1292-1298 https://doi.org/10.1542/peds.105.6.1292
  17. Sastry, P. 1985. Lipids of nervous tissue: Composition and metabolism. Prog. Lipid Res. 24, 69-176 https://doi.org/10.1016/0163-7827(85)90011-6
  18. Sinclair, A. J. 1975. Incorporation of radioactive polyunsaturated fatty acids into liver and brain of developing rat. Lipids 10, 120-123
  19. Stewart, C. A. and R. Morris. 1993. The water maze. pp. 107-122, In Sagal, A. (ed.), Behavioral Neuroscience: A Practical Approach, Vol. 1, Oxford University Press, New York
  20. Su, H. M., M. C. Huang, N. M. R. Saad, P. W. Nathanielsz, and J. T. Brenna. 2001. Fetal baboons convert 18:3n-6 to 22:6n-3 in vivo: a stable isotope tracer study. J. Lipid Res. 42, 581-586
  21. Uauy, R. D., D. G. Birch, E. E. Birch, J. E. Tyson, and D. R. Hoffman. 1990. Effect of dietary omega-3-fatty-acids on retinal function of very-low-birth-the-weight neonates. Ped. Res. 28, 485-492 https://doi.org/10.1203/00006450-199011000-00014
  22. Valenzuela, A., R. Van Bernhardi, V. Valenzuela, G. Ramirez, R. Alarcon, J. Sanhueza, and S. Nieto. 2004. Supplementation of female rats with alpha- linolenic acid or docosahexaenoic acid leads to the same omega-6/ omega-3 LC-PUFA accretion in mother tissues and in fetal and newbron brains. Ann. Nutr. Metab. 48, 28-35 https://doi.org/10.1159/000075082
  23. Weisinger, H. S., A. J. Vingrys, B. V. Bui, and A. J. Sinclair. 1999. Effect of dietary n-3 deficiency and repletion in the guinea pig retina. Invest. Ophthalmol. Vis. Sci. 40, 327-338
  24. Weisinger, H. S., J. A. Armitage, A. J. Sinclair, A. J. Vingrys, P. Burns, and R. S. Weisinger. 2001. Perinatal omega-3 fatty acid deficiency affects blood pressure later in life. Nature Med. 7, 258-259 https://doi.org/10.1038/85354
  25. Weisinger, H. S., J. A. Armitage, B. G, Jeffrey, D. C. Mitchell, T. Moriguchi, A. J. Sinclair, R. S. Weisinger, and N. Salem. 2002. Retinal sensitivity loss in third-generation n-3 PUFA-deficient rats. Lipids 37, 759-765 https://doi.org/10.1007/s11745-002-0958-3
  26. Willatts, P., J. S. Forsyth, M. K. DiModugno, S. Varma, and M. Colvin. 1998. Influence of long-chain polyunsaturated fatty acids on infant cognitive function. Lipids 33, 973-980 https://doi.org/10.1007/s11745-998-0294-7

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