The Effects of Hydroxyapatite-Chitosan Membrane on Bone Regeneration in Rat Calvarial Defects

  • Shin, Jung-A (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University, College of Dentistry) ;
  • Choi, Jung-Yoo (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University, College of Dentistry) ;
  • Kim, Sung-Tae (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University, College of Dentistry) ;
  • Kim, Chang-Sung (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University, College of Dentistry) ;
  • Lee, Yong-Keun (Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University, College of Dentistry) ;
  • Cho, Kyoo-Sung (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University, College of Dentistry) ;
  • Chai, Jung-Kiu (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University, College of Dentistry) ;
  • Kim, Chong-Kwan (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University, College of Dentistry) ;
  • Choi, Seong-Ho (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University, College of Dentistry)
  • Published : 2009.08.15

Abstract

Purpose: Recently, interest in chitosan has increased due to its excellent biological properties such as biocompatibility, antibacterial effect, and rapid healing capacity. On the other hand, hydroxyapatite is used as a bone substitute in the fields of orthopedics and dentistry. The hydroxyapatite-chitosan (HA-CS) complex containing hydroxyapatite nanoparticles was developed for synergy of both biomaterials. The objective of this study was to evaluate the effect of hydroxyapatite (HA)-chitosan (CS) membrane on bone regeneration in the rat calvarial defect. Methods: Eight-millimeter critical-sized calvarial defects were created in 70 male Sprague-Dawley rats. The animals were divided into 7 groups of 10 animals and received either 1) chitosan (CS) 100% membrane, 2) hydroxyapatite (HA) 30%/CS 70% membrane, 3) HA 30%/CS 70%, pressed membrane, 4) HA 40%/CS 60% membrane, 5) HA 50%/CS 50% membrane, 6) HA 50%/CS 50%, pressed membrane, or 7) a sham . surgery control. The amount of newly formed bone from the surface of the rat calvarial defects was measured using histomorphometry, following 2- or 8- week healing intervals. Results: Surgical implantation of the HA - CS membrane resulted in enhanced local bone formation at both 2 and 8 weeks compared to the control group. The HA - CS membrane would be significantly more effective than the chitosan membrane in early bone formation. Conclusions: Concerning the advantages of biomaterials, the HA-CS membrane would be an effective biomaterial for regeneration of periodontal bone. Further studies will be required to improve the mechanical properties to develop a more rigid scaffold for the HA-CS membrane.

Keywords

References

  1. Magnusson I, Batich C, Collins BR.New attachment formation following controlled tissue regeneration using biodegradable membranes. J Periodontol 1998;59:1-6 https://doi.org/10.1902/jop.1988.59.1.1
  2. Aquirre-Zorzano LA, Estefania-Cundin E, GilLozano J. Periodontal regeneration of infrabony defectsusing resorbable membrane: determinant of the healing response. An observational clinical study. Int J Periodontics Rest Dent 1999;19:363-371
  3. Zitzmann NU, Naef R and Schaurer P. Resorbable versus nonresorbable membranes in combination with Bio-Oss for guided bone regeneration. Int J Oral Maxillofac Implant 1997;12:844-852
  4. Becker W, Becker BD, Mellonig J. A prospective multi- center study evaluation periodontal regeneration for class II furcation invasions and intrabony defects after treatment with a bioabsorbable barrier membrane: 1-year results, J Periodontol 1996;67:641-649 https://doi.org/10.1902/jop.1996.67.7.641
  5. Hong SB, Kwon YH, Lee MS, Herr Y. Comparative study on tissue responses of 3 resorbable membranes in rats. J Korean Acad Periodontol 2002;32:475-488 https://doi.org/10.5051/jkape.2002.32.3.475
  6. Amano K, Ito E. The action of lysozyme on partially deacetylate chitin. European J Biochem 1978;85:97-104 https://doi.org/10.1111/j.1432-1033.1978.tb12216.x
  7. Pangburn SH, Trescony PV, & Heller J. Lysozyme degradation of partially deacetylated chitin, its films and hydrogels. Biomaterials 1982;3:105-108 https://doi.org/10.1016/0142-9612(82)90043-6
  8. Reynolds, B.L. Wound healing III: Artificial maturation of arrested regenerate with an acetylated amino sugar. The American Surgeon 1960;26:113-117
  9. Kim OS, Chung HJ. Effects of chitosan on human gingival fibroblasts in vitro. J Korean Acad Periodontol 2002;32:235-247 https://doi.org/10.5051/jkape.2002.32.1.235
  10. Paik JW, Lee HJ, Yoo YJ. The effects of chitosan on the human periodontal ligament fibroblasts in vitro. J Korean Acad Periodontol 2001;31:823-832 https://doi.org/10.5051/jkape.2001.31.4.823
  11. Park JS, Choi SH, Moon IS. Eight-week histological analysis on the effect of chitosan on surgically created one-wall intrabony defects in beagle dogs. J Clin Periodontol 2003;30:443-453 https://doi.org/10.1034/j.1600-051X.2003.10283.x
  12. Salata LA, Craig GT, Brook IM. Bone healing following the use of hydroxyapatite or ionomericbone substitutes alone or combined with a guided bone regeneration technique: an animal study. Int J Oral Maxillofac Implants 1998;13:44-51
  13. Zhang C, Hu YY,Cui FZ, Zhang SM, Ruan DK. A study on a tissue-engineered bone using rhBMP-2 induced periosteal cells with a porous nano-hydroxyapatite/collagen /poly(L-lactic acid) scaffold. Biomed Mater 2006;2:56-62
  14. Wang HL, MacNeal RL, Shieh AT. Utilization of a resorbable collagen membrane in repairing gingival recession defects. Pract Periodont Aesthet Dent 1996;8:441-448
  15. Wang HL, O'Neal RB, Tomas CL. Evaluation of and absorbale collagen membrane in treating class II furcation defects. J Periodontol 1994;65:1029-1036 https://doi.org/10.1902/jop.1994.65.11.1029
  16. Polson AM, Garrett S, Stoller NH. Guided tissue regeneration in human furcation defects after using biodegradable barrier. : A multi-center feasibility study. J Periodontol 1995;66:377-385 https://doi.org/10.1902/jop.1995.66.5.377
  17. Bouchard P, Giovannoli JL, Mattout C. Clinical evaluation of a bioabsorble regenerative material in mandibular class II furcation therapy. J Clin Periodontol 1997;24:511-518 https://doi.org/10.1111/j.1600-051X.1997.tb00220.x
  18. Jung UW, Song KY, Kim CS, Lee YK, Cho KS, Kim CK, Choi SH. Effects of a chitosan membrane coated with polylactic and polyglycolic acid on bone regeneration in a rat calvarial defect. Biomed Mater 2007;3:S101-105
  19. Webster TJ, Ergun C, Doremus RH. Enhanced functions of osteoblasts on nanophase ceramics. Biomaterials 2000;21:1803-1810 https://doi.org/10.1016/S0142-9612(00)00075-2
  20. Zhang YF, Cheng XR, Chen Y. Three-dimensional nanohydroxyapatite/ chitosan scaffolds as potential tissue engineered periodontal tissue. J Biomater Appl 2007;21:333-349 https://doi.org/10.1177/0885328206063853
  21. Kong L, Gao Y, Cao W. Preparation and characterization of nano-hydroxyapatite/chitosan composite scaffolds. J Biomed Mater Res A 2005;75:275-282
  22. Guobao W, Peter X. Ma. Structure and properties of nano-hydroxyapatite/polymer composite scaffolds for bone tissue engineering. Biomaterials 2004;25:4749–4757 https://doi.org/10.1016/j.biomaterials.2003.12.005
  23. Schmitz JP, Hollinger JO: The critical size defect as an experimental model for craniomandibulofacial nonunoins, Clin Orthop 1986;205:299-308
  24. Caton J, Mota L, Gandini L. Non-human primate models for testing the efficacy and safety of periodontal regeneration procedures. J Periodontol 1994;65:1143-1150 https://doi.org/10.1902/jop.1994.65.12.1143
  25. Selvig KA. Discussion: Animal models in reconstructive therapy. J Periodontol 1994;65:1169-1172 https://doi.org/10.1902/jop.1994.65.12.1169
  26. Jung UW, Suh JJ, Choi SH. The bone regenerative effects of chitosan on the calvarial critical size defect in Sprague-Dawley rats. J Korean Acad Periodontol 2000;30:851-868 https://doi.org/10.5051/jkape.2000.30.4.851
  27. Pang EK, Paik JW, Kim SK. Effects of chitosan on human periodontal ligament fibroblasts in vitro and on bone formation in rat calvarial defects J Periodontol 2005;76:1526-1533 https://doi.org/10.1902/jop.2005.76.9.1526
  28. Oonishi H, Hench LL, Wilson J. Comparative bone growth behavior in granules of bioceramic materials of various sizes. J Biomed Mater Res 1999;44:31-43 https://doi.org/10.1002/(SICI)1097-4636(199901)44:1<31::AID-JBM4>3.0.CO;2-9
  29. Manjubala I, Sivakumar M, Sureshkumar RV. Bioactivity and osseointegration study of calcium phosphate ceramic of different chemical composition. J Biomed Mater Res 2002;63:200-208 https://doi.org/10.1002/jbm.10128
  30. Liljensten E, Adolfsson E, Strid KG. Resorbable and nonresorbable hydroxyapatite granules as bone graft substitutes in rabbit cortical defects. Clin Implant Dent Relat Res 2003;5:95-101 https://doi.org/10.1111/j.1708-8208.2003.tb00190.x
  31. Andrade JC, Camilli JA, Kawachi EY. Behavior of dense and porous hydroxyapatite implants and tissue response in rat femoral defects. J Biomed Mater Res 2002;62:30-36 https://doi.org/10.1002/jbm.10242
  32. Takeshita F, Ayukawa Y, lyama S. Histological comparison of early wound healing following dense hydroxyapatite granule grafting and barrier placement in surgically-created bone defects neighboring implants. J Periodontol 1997;68:924-932 https://doi.org/10.1902/jop.1997.68.10.924