Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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The Effect of Injinho-tang Extract on Hepatocellular Carcinogenesis and Hepatic Cirrhosis Induced by Diethylnitrosamine and CCl4 in Rats

인진호탕(茵蔯蒿湯) 추출액이 Diethylnitrosamine과 CCl4로 유발된 흰쥐의 간암(肝癌) 형성과 간경변(肝硬變)에 미치는 영향

  • Lee, Jong-Bum (Department of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Heo, Rae-Kyong (Department of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Seung, Kee-Moon (Department of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Moon, Goo (Department of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Lee, Jong-Deok (Department of Radiology, Won-kwang University Hospital) ;
  • Won, Jin-Hee (Department of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University)
  • 이종범 (원광대학교 한의과대학 내과학교실) ;
  • 허래경 (원광대학교 한의과대학 내과학교실) ;
  • 성기문 (원광대학교 한의과대학 내과학교실) ;
  • 문구 (원광대학교 한의과대학 내과학교실) ;
  • 이종덕 (원광대학교 광주병원 영상의학과) ;
  • 원진희 (원광대학교 한의과대학 내과학교실)
  • Published : 2009.09.30
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Abstract

Injinho-tang(IJ) has been used for the treatment of hepatobiliary diseases. This study was performed to observe the effect of IJ extract on the hepatocellular carcinogenesis and hepatic cirrhosis induced by Diethylnitrosamine(DENA) and $CCl_4$ in Rats. Experimental groups were divided into two ; 8th and 12th week group, and subdivided into four; normal group(Nor), hepatocellular cancer and hepatic cirrhosis inducing control group(Con), and IJ extract 260mg/kg/day(IJA) or 520mg/kg/day(IJB) administered groups to Con. The results obtained are as follows: The body weight was decreased in the Con, IJA and IJB compared with the Nor from the 2nd week to the 12th week. The weight of liver and the weight of liver/100g body weight were decreased significantly in Con, IJA and IJB compared with the Nor. The activities of aspartate aminotransferase(AST) and alanine aminotransferase(ALT) were significantly increased in the Con compared with Nor, but decreased in the IJA and IJB compared with Con from the 8th week group. The activities of alkaline phosphatase(ALP), lactacte dehydrogenase (LDH) and alpha fetoprotein(AFP) were increased significantly in the Con compared with Nor, but decreased in the IJA and IJB compared with Con. The activities of superoxide dismutase(SOD) were decreased in the IJA and IJB compared with Con, but the activities of catalase were increased in the IJA and IJB compared with Con. According to the light and electron microscopical observation, IJA and IJB improved the morphological and histopathological changes of the liver injured by DENA and $CCl_4$. The number of hepatic p53 positive cells was decreased in the IJA and IJB compared with Con. These results suggest that administration of IJ extract suppress or retard DENA and $CCl_4$-induced hepatocelluar carcinogenesis and hepatic cirrhosis in rats.

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