한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제13권2호
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  • Pages.89-95
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  • 2009
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
권호 표지

생쥐의 초기 두개악안면 발생 중 p63의 발현 양상

Expression of p63 during Early Craniofacial Development of the Mouse Embryo

  • 호소야 아카히로 (연세대학교 치과대학 구강생물학교실, BK21 치의과학 사업단, 구강악안면경조직 재생연구센터, 구강과학연구소) ;
  • 이종민 (연세대학교 치과대학 구강생물학교실, BK21 치의과학 사업단, 구강악안면경조직 재생연구센터, 구강과학연구소) ;
  • 김지연 (연세대학교 치과대학 구강생물학교실, BK21 치의과학 사업단, 구강악안면경조직 재생연구센터, 구강과학연구소) ;
  • 정한성 (연세대학교 치과대학 구강생물학교실, BK21 치의과학 사업단, 구강악안면경조직 재생연구센터, 구강과학연구소) ;
  • 조성원 (연세대학교 치과대학 구강생물학교실, BK21 치의과학 사업단, 구강악안면경조직 재생연구센터, 구강과학연구소)
  • Akihiro, Hosoya (Division in Anatomy and Developmental Biology, Dept. of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 Project, Yonsei University College of Dentistry) ;
  • Lee, Jong-Min (Division in Anatomy and Developmental Biology, Dept. of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 Project, Yonsei University College of Dentistry) ;
  • Kim, Ji-Youn (Division in Anatomy and Developmental Biology, Dept. of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 Project, Yonsei University College of Dentistry, Dept. of Oral Histology, Matsumoto Dental Univeristy) ;
  • Jung, Han-Sung (Division in Anatomy and Developmental Biology, Dept. of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 Project, Yonsei University College of Dentistry) ;
  • Choi, Sung-Won (Division in Anatomy and Developmental Biology, Dept. of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 Project, Yonsei University College of Dentistry)
  • 발행 : 2009.06.30
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초록

p63은 다양한 상피 조직의 줄기세포와 전구세포에 존재한다는 사실이 잘 알려져 있으나, 치아 형성, 특히 사기질과 뿌리 형성시기에서의 p63 위치느 ㄴ아직 연구해야 할 과제로 남아 있다. 본 연구에서는 p63이 치아 발생 동안 치아상피에 편재하여 나타나는 것을 면역조직화학 기법을 이용하여 확인하였다. p63은 피부, 모낭, 구강점막 그리고 턱밑샘 도관을 포함하는 상피의 바닥층과 바닥위층에 위치하였다. 그러나 치아 부위에서는 치아관의 모든 세포, 사기질기관, 헤르트비히 뿌리상피집 그리고 말라세쯔 상피잔사에 p63이 관찰되었다. 이 결과는 치아 발생 중 p63이 줄기세포 유지 외에도 다른 기능을 한다는 사실을 보여준다.

p63 has been demonstrated to localize in stem cells and precursor cells of various epithelial tissues previously, but the localization of p63 throughout tooth formation, particularly during the enamel and root formation stages, remains to be adequately characterized. Therefore, in this study, we have demonstrated, via immunohistochemical methods, that p63 is ubiquitously expressed in the dental epithelium during tooth development. p63 was detected in the basal and suprabasal layers of the epithelia, including the skin, hair follicles, oral mucosa, and submandibular ducts. However, in the tooth region, all cells of the dental lamina, enamel organ, Hertwig's epithelial root sheath (HERS), and epithelial cell rests of Malassez (ERM) evidenced immunoreactivity for p63. These results indicate that p63 may perform different roles, other than stem cell maintenance, in tooth development.

키워드

참고문헌

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