Journal of Preventive Medicine and Public Health

The Korean Society for Preventive Medicine (대한예방의학회)
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Determination of Appropriate Sampling Time for Job Stress Assessment: the Salivary Chromogranin A and Cortisol in Adult Females

  • Hong, Ran-Hi (Department of Preventive Medicine, Chung-Ang University College of Medicine) ;
  • Yang, Yun-Jung (Department of Preventive Medicine, Chung-Ang University College of Medicine) ;
  • Kim, Sang-Yon (Department of Preventive Medicine, Chung-Ang University College of Medicine) ;
  • Lee, Won-Young (Department of Preventive Medicine, Chung-Ang University College of Medicine) ;
  • Hong, Yeon-Pyo (Department of Preventive Medicine, Chung-Ang University College of Medicine)
  • Published : 2009.07.31
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Abstract

Objectives : This study was conducted to determine the appropriate sampling time of the salivary stress markers, chromogranin A (CgA) and cortisol as objective indices of job stress assessment in adult females. Methods : The subjects were 20${\sim}$39-year-old women (13 office workers, 11 sales-service workers, and 11 college students) who were eligible for the study and free of acute and chronic medical conditions. Salivary CgA and cortisol levels were determined by enzyme-linked immunosorbent assay (ELISA). Saliva samples were collected (2 $m{\ell}$ each) at 7:00, 8:00, 10:30, 12:00, 17:30, and 22:30 on a typical day. Salivary CgA and cortisol levels, according to sampling time, were compared among the three groups using general linear model. The full version of the Korean Occupational Stress Scale (KOSS), which includes socioeconomic characteristics, health behavior, workrelated characteristics, and BMI, was used to access the subjects' job stress. Multiple regression analysis of the job stressors identified by the KOSS was performed on salivary CgA and cortisol levels. Results : The salivary CgA level peaked at 7:00 (time of awakening), then decreased and were maintained at a low level throughout the day, and increased slightly at 17:30. The salivary cortisol level increased steeply within the 1st hour after awakening, followed by a gradual decrease by 12:00, and was then maintained at a low level throughout the day. The salivary cortisol levels of subjects who worked ${\leq}$5 days per week and graduated from the university were significantly lower at 8:00 (p=0.006). The salivary cortisol levels of non-smokers were significantly lower at 7:00 p=0.040) and 8:00 (p=0.003) compared to smokers. There were no significant differences in salivary CgA and cortisol levels at 10:30 and 12:00 in general characteristics. The regression coefficients on salivary CgA level were significant with interpersonal conflict at 17:30 and job insecurity at 22:30. Regression coefficients on salivary cortisol level were significant with organizational system and total job stressors at 17:30. Conclusions : We suggest that the appropriate sampling times for the salivary stress markers, CgA and cortisol, are at 7:00 (time of awakening), 8:00 (1 hour after awakening), 17:30 (early evening), and 22:30 (before sleep).

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References

  1. Rice PL. Stress and Health. Pacific Grove: Brooks/Cole Publishing Company; 1999. p.159-281
  2. Chang SJ, Koh SB, Kang DM, Kim SA, Kang MG, Lee CG, et al. Developing an occupational stress scale for Korean employees. Korean J Occup Environ Med 2005; 17(4): 297-317. (Korean)
  3. Karasek R, Brisson C, Kawakami N, Houtman I, Bongers P, Amick B. The job content questionnaire (JCQ): An instrument for internationally comparative assessments of psychosocial job characteristics. J Occup Health Psychol 1998; 3(4): 322-355 https://doi.org/10.1037/1076-8998.3.4.322
  4. Shigeyama C, Ansai T, Awano S, Soh I, Yoshida A, Hamasaki T, et al. Salivary levels of cortisol and chromogranin A in patients with dry mouth compared with age-matched controls. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008; 106(6): 833-839 https://doi.org/10.1016/j.tripleo.2008.07.005
  5. Lee T, Shimizu T, Iijima M, Obinata K, Yamashiro Y, Nagasawa S. Evaluation of psychosomatic stress in children by measuring salivary chromogranin A. Acta Paediatr 2006; 95(8): 935-939 https://doi.org/10.1080/08035250500538940
  6. Nakane H, Asami O, Yamada Y, Harada T, Matsui N, Kanno T, et al. Salivary chromogranin A as an index of psychosomatic stress response. Biochem Res 1998; 19(6): 401-406
  7. Kirschbaum C, Hellhammer DH. Salivary cortisol in psychoneuroendocrine research: Recent developments and applications. Psychoneuroendocrinology 1994; 19(4): 313-333 https://doi.org/10.1016/0306-4530(94)90013-2
  8. Kirschbaum C, Hellhammer DH. Salivary cortisol in psychobiological research: An overview. Neuropsychobiology 1989; 22(3): 150-169 https://doi.org/10.1159/000118611
  9. Den R, Toda M, Nagasawa S, Kitamura K, Morimoto K. Circadian rhythm of human salivary chromogranin A. Biomed Res 2007; 28(1): 57-60 https://doi.org/10.2220/biomedres.28.57
  10. DeSantis AS, Adam EK, Doane LD, Mineka S, Zinbarg RE, Craske MG. Racial/ethnic differences in cortisol diurnal rhythms in a community sample of adolescents. J Adolesc Health 2007; 41(1): 3-13 https://doi.org/10.1016/j.jadohealth.2007.03.006
  11. Turner RJ, Avison WR. Status variations in stress exposure: Implications for the interpretation of research on race, socioeconomic status, and gender. J Health Soc Behav 2003; 44(4): 488-505 https://doi.org/10.2307/1519795
  12. Edwards S, Clow A, Evans P, Hucklebridge F. Exploration of the awakening cortisol response in relation to diurnal cortisol secretory activity. Life Sci 2001; 68(18): 2093-2103 https://doi.org/10.1016/S0024-3205(01)00996-1
  13. Buchanan TW, Kern S, Allen JS, Tranel D, Kirschbaum C. Circadian regulation of cortisol after hippocampal damage in humans. Biol Psychiatry 2004; 56(9): 651-656 https://doi.org/10.1016/j.biopsych.2004.08.014
  14. Schulz P, Kirschbaum C, PruBner J, Hellhammer D. Increased free cortisol secretion after awakening in chronically stressed individuals due to work overload. Stress Med 1998; 14(2): 91-97 https://doi.org/10.1002/(SICI)1099-1700(199804)14:2<91::AID-SMI765>3.0.CO;2-S
  15. Kang DM, Son BM, Koh SB, Son M, Kim J, Jang J, et al. Effects of physical workload on salivary cortisol level. Korean J Occup Environ Med 2004; 16(1): 57-69. (Korean)
  16. Bauer ME, Vedhara K, Perks P, Wilcock GK, Lightman SL, Shanks N. Chronic stress in caregivers of dementia patients is associated with reduced lymphocyte sensitivity to glucocorticoids. J Neuroimmunol 2000; 103(1): 84-92 https://doi.org/10.1016/S0165-5728(99)00228-3
  17. Pruessner JC, Hellhammer DH, Kirschbaum C. Burnout, perceived stress, and cortisol responses to awakening. Psychosom Med 1999; 61(2): 197-204 https://doi.org/10.1097/00006842-199903000-00012
  18. Gaviao MB, Engelen L, van der Bilt A. Chewing behavior and salivary secretion. Eur J Oral Sci 2004; 112(1): 19-24 https://doi.org/10.1111/j.0909-8836.2004.00105.x
  19. Center for Drug Evaluation and Research. Guidance for industry: Bioanalytical methods validation for human studies. Rockville: Center for Drug Evaluation and Research; 1998 [cited 2008 Mar 17]. Available from: URL:http://www.pharmanet.com.br/pdf/2578dft.pdf
  20. Grossia G, Perskia A, Evengardb B, Blomkvistc V, Orth-Gomerd K. Physiological correlates of burnout among women. J Psychosom Res 2003; 55(4): 309-316 https://doi.org/10.1016/S0022-3999(02)00633-5
  21. Toda M, Den R, Nagasawa S, Kitamura K, Morimoto K. Relationship between lifestyle scores and salivary stress markers cortisol and chromogranin A. Arch Environ Occup Health 2005; 60(5): 266-269 https://doi.org/10.3200/AEOH.60.5.266-269
  22. Rystedt LW, Cropley M, Devereux JJ, Michalianou G. The relationship between longterm job strain and morning and evening saliva cortisol secretion among white-collar workers. J Occup Health Psychol 2008; 13(2): 105-113 https://doi.org/10.1037/1076-8998.13.2.105
  23. Steptoe A, Cropley M, Griffith J, Kirschbaum C. Job strain and anger expression predict early morning elevations in salivary cortisol. Psychosom Med 2000; 62 (2): 286-292 https://doi.org/10.1097/00006842-200003000-00022
  24. Ruis MA, Te Brake JH, Engel B, Ekkel ED, Buist WG, Blokhuis HJ, et al. The circadian rhythm of salivary cortisol in growing pigs: Effect of age, gender and stress. Physiol Behav 1997; 62(3): 623-630 https://doi.org/10.1016/S0031-9384(97)00177-7