Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Study on Development of Vitrified Bovine Immature Oocytes Following ICSI

  • Park, Ji-Hoon (College of Veterinary Medicine, Chungnam National University) ;
  • Rhee, Man-Hee (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Sang-Keun (College of Veterinary Medicine, Chungnam National University)
  • Published : 2009.06.30
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Abstract

In the present study, effects of concentration of cryoprotectant solutions on the nuclear maturation of vitrifiedthawed bovine oocytes were examined. Also, the developmental capacity of vitrified-thawed immature oocytes following ICSI was investigated. Oocytes were cultured in TCM-199 medium supplemented with 5% FBS at $38^{\circ}$C in 5% $CO_2$ and air. The in vitro maturation rate of vitrified oocytes was 24.5 ${\pm}$ 4.2%. The in vitro maturation rate of vitrified oocytes was lower than that of the control (72.0 ${\pm}$ 3.5%, p<0.05). The in vitro maturation rate of vitrified${\sim}$thawed oocytes incubated in TCM-199 medium supplemented with 1.0${\sim}$5.0 ug CB were 26.7 ${\pm}$ 3.2%, 35.7 ${\pm}$ 3.2%, 54.0 ${\pm}$ 3.0%, 42.5 ${\pm}$ 3.6%, respectively. The in vitro maturation rate (57.0 ${\pm}$ 3.0%) of the vitrified-thawed oocytes treated with 3.0 ${\mu}$g CB for 20 min was the highest of all vitrification groups, although the maturation rate were significantly (p<0.05) lower than those of fresh oocytes. The in vitro maturation rates of the vitrified-thawed (with EDS and EDT) oocytes were 53.8 ${\pm}$ 3.4%, 51.1 ${\pm}$ 3.5%, respectively. This results were lower than the control group (72.0 ${\pm}$ 3.0%). The in vitro developmental rates of the vitrified-thawed oocytes following ICSI were 28.6 ${\pm}$ 4.5%, 25.6 ${\pm}$ 4.3%, respectively. This results were lower than the control group (40.0 ${\pm}$ 4.0%).

Keywords

References

  1. Candy CJ, Wood M, Whittingham DG, Merriman JA and Choudhury N. 1994. Cryopreservation of immature mouse oocytes. Hum. Reprod. 9:1738-1742
  2. Cuello C, Sntonia MG, Parrilla I, Tornel J, Vazquez JM, Roca J, Berthelot F, Martinat-Botte F and Martinez EA. 2004. Vitrification of porcine embryos at various development stages using different ultra-rapis cooling procedures. Theriogenology 62:353-361 https://doi.org/10.1016/j.theriogenology.2003.10.007
  3. Fabbri R, Porcu E, Marsella T, Roccetta G, Venturoli S and Flamigni C. 2001. Human oocytes cryopreservation: New perspectives regarding oocyte survival, Hum. Reprod. 16:411-416 https://doi.org/10.1093/humrep/16.3.411
  4. Fujihira T, Kishida R and Fukui Y. 2004. Developmental capacity of vitrified immature porcine oocytes following ICSI:Effects of cytochalasin B and cryoprotectants. Cryobiology 49:286-290 https://doi.org/10.1016/j.cryobiol.2004.08.004
  5. Hamlett DK, Franken DR, Cronje HS and Luus H. 1989. Murine oocyte cryopreservation: Comparison between fertilization success rates of fresh and frozen metaphase I and II oocytes. Arch. Andol. 23:27 https://doi.org/10.3109/01485018908986785
  6. Isachenko V, Soler C, Isachenko E, Perez-Sanchez F and Grishchenko V. 1998. Vitrification of immature porcine oocytes:effects of lipid droplets, temperature, cytoskeleton, and addition and removal of cryoprotectant. Cryobiology 36: 250-253 https://doi.org/10.1006/cryo.1998.2079
  7. Kasai M, Komi JH, Takakamo A, Tssnoda H. Sakurai T and Machida T. 1990. A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability. J. Reprod. Fertil. 89:91-97 https://doi.org/10.1530/jrf.0.0890091
  8. Leibo SP and Oda K. 1993. High survival of mouse zygote and embryos cooled rapidly or slowly in ethylene glycol plus polyvinyl pyrrolidone. Cryo-Letters 14:133-144 https://doi.org/10.1006/cryo.1996.1981
  9. Mazur P. 1972. Cryobiology; The Freezing of Biological Systems. Sci. Washington D.C., 168:939-949 https://doi.org/10.1126/science.168.3934.939
  10. Mullen SF, Agca Y, Broermann DC, Jenkins CL, Johnson CA and Critser JK. 2004. The effect of osmotic stress on the metaphase II spindle of human oocytes, and the relevance to cryopreservation. Hum. Reprod. 19:1148-1154 https://doi.org/10.1093/humrep/deh201
  11. Rall WF and Fahy GM. 1985. Ice-free cryopreservation of mouse embryos at -196${^{\circ}C}$ by vitrification. Nature 313:573-575 https://doi.org/10.1038/313573a0
  12. Rall WF. 1992. Cryopreservation of oocytes and embryos methods and applications. Anim. Reprod. 28:237-245 https://doi.org/10.1016/0378-4320(92)90110-Y
  13. Renard JP, Nguyen BX and Gamier V. 1984. Two-step freezing of two-cell rabbit embryos after partial dehydration at room temperature. J. Reprod. Fert. 71:573-580 https://doi.org/10.1530/jrf.0.0710573
  14. Robinski B, Arav A and Devires AL. 1991. Cryopreservation of oocytes using directional cooling and antifreeze glycoproteins. Cryo-Lettters 12:93-106
  15. Schmidt M, Hyttle P, Grece T and Avery B. 1993. Ultrastructure of frozen thawed bovine in vitro matured oocytes. Theriogenology 39:304 https://doi.org/10.1016/0093-691X(93)90159-3
  16. Suzuki T and Nishikata Y. 1992. Fertilization and cleavage of frozen thawed bovine oocytes by one step dilution methos in vitro. Theriogenology 37:306 https://doi.org/10.1016/0093-691X(92)90375-2
  17. Toth TL, Jones HW, Baka SC, Muasher S, Veeck LL and Lanzendorf SE. 1994. Fertilization and in vitro development of cryopreserved human prophase I oocytes. Fertil. Steril. 61:891-894 https://doi.org/10.1016/S0015-0282(16)56702-8
  18. Vajta G, Holm P, Kuwayama M, Booth PJ, Jacobsen A, Greve T and Callesen H. 1998. Open pulled straw (OPS) vitrification:A new way to reduce cryoinjuries of bovine ova and embryos. Mol. Reprod. Dev. 51:53-58 https://doi.org/10.1002/(SICI)1098-2795(199809)51:1<53::AID-MRD6>3.0.CO;2-V
  19. van Blerkom J. 1989. Maturation at high frequency of germinal vesicle-stage mouse oocyte after cryopreservation: Alterations in cytoplasmic, nuclear, nucleolar and chromosomal structure and organization associated with vitrification. Human Reprod. 4:883-89
  20. van der Elst JC, Nerinckx SS and van Steirteghem AC. 1993. Slow and ultrarapid freezing of fully grown germinal vesicle-stage mouse oocytes: Optimization of survival rate out-weighted by defective blastocyst formation. J. Assist. Reprod. Gene 10:202-212 https://doi.org/10.1007/BF01239222
  21. Vicente JS and Garcia-Ximenez F. 1994. Osmotic and cryoprotective effects of a mixture of DMSO and ethylene glycolon rabbit morulae. Theriogenology 42:1205-1215 https://doi.org/10.1016/0093-691X(94)90869-9