사상체질의학회지 (Journal of Sasang Constitutional Medicine)

  • 제21권3호
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  • Pages.138-153
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  • 2009
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  • 1226-4075(pISSN)
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  • 2287-786X(eISSN)
사상체질의학회 (The Society Of Sasang Constitutional Medicine)
권호 표지

$\beta$-Amyloid로 유도된 신경독성에 대한 열다한소탕(熱多寒少湯) 가감방(加感方)의 항(抗)치매효과

Protection effect of New-Yeolda-Hanso tang against $\beta$-Amyloid Induced Cytotoxicity in NGF-differentiated PC12 Cells

  • 배나영 (대전대학교 한의과대학 사상체질과) ;
  • 양현옥 (한국과학기술 연구원 강릉분원 천연물 소재 연구센터) ;
  • 안택원 (대전대학교 한의과대학 사상체질과)
  • Bae, Na-Young (Dept. of Sasang Constitution Medicine, College of Oriental Medicine, Daejeon Univ.) ;
  • Yang, Hyun-Ok (Natural Product Research Center, Korea Institute of Science and Technology) ;
  • Ahn, Taek-Won (Dept. of Sasang Constitution Medicine, College of Oriental Medicine, Daejeon Univ.)
  • 투고 : 2009.10.24
  • 심사 : 2009.12.02
  • 발행 : 2009.12.31
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초록

1. Objectives: Yeolda-Hanso tang (YH) has long been used as traditional herbal formula in Korea as various diseases. Now we modified Yeolda-Hanso tang (YH) for neurodegenerative diseases treatment and named New-Yeolda-Hanso tang (NYH). We investigated neuroprotective effects of NYH on NGF-differentiated PC12 cells cytotoxicity induced by $\beta$-Amyloid peptide (A$\beta$25-35) and evaluated the ability of NYH to prevent and treat for neurodegenerative diseases via autophagy enhancement. 2. Methods and Results: 1) Protective effect of NYH on PC12 cells cytotoxity induced by A$\beta$25-35. PC12 cells survival was measured by MTT and lactate dehydrogenase (LDH) assay. $20{\mu}M$ $\beta$-Amyloid peptide (A$\beta$25-35) induced cytotoxicity on NGF-differentiated PC12 cells. NYH attenuated the cytotoxic effects of A$\beta$25-35 in a dose-dependent manner. 2) Pharmacological induction of Autophagy by NYH in PC12 cells Autophagy induction and activation was measured by immunoblot assay. Marker of autophagy, LC3 II expression and the ratio of LC3-II/I was slightly increased in the protein treated with YH, and significantly augmented in the protein treated with NYH. NYH-induced increase of LC3-II protein level was inhibited by 3MA. 3) Induction of Autophagy by NYH on A$\beta$25-35-induced injury in PC12 cells In MTT assay, $100{\mu}g/ml$ re-treated NYH attenuated $20{\mu}M$ A$\beta$25-35-induced cytotoxicity in PC12 cells. Protection effect of NYH was blocked by autophagy inhibitor 3MA. In immunoblot assay, $1200{\mu}g/ml$ pre-treated NYH activated autophagy in $20{\mu}M$ A$\beta$25-35-induced cytotoxicity in PC12 cells. The observed effect was partially blocked by 3MA. 3. Conclusions: All the results indicated that NYH possesses neuroprotective potential partially mediated by autophagy enhancement and NYH may be considered to be a promising new herbal formula to prevent and treat for neurodegenerative diseases including Alzheimer's disease (AD).

키워드

과제정보

연구 과제 주관 기관 : 한국과학기술원

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