Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Selection of Rearing Media, Proper Temperature and Period for Storage of Sterile Lucilia sericata (Diptera: Calliphoridae) Eggs and Larvae

무균 구리금파리 유충 생산용 배지의 선발과 알 및 유충의 저장을 위한 온도 및 기간

  • Jang, Sin-Ae (Institute of Agriculture and Life Science / Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang Nat'l Univ.) ;
  • Yun, Ji-Eun (Institute of Agriculture and Life Science / Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang Nat'l Univ.) ;
  • Park, Chung-Gyoo (Institute of Agriculture and Life Science / Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang Nat'l Univ.)
  • 장신애 (경상대학교 농업생명과학연구원, 대학원 응용생명과학부) ;
  • 윤지은 (경상대학교 농업생명과학연구원, 대학원 응용생명과학부) ;
  • 박정규 (경상대학교 농업생명과학연구원, 대학원 응용생명과학부)
  • Published : 2009.06.30
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Abstract

Rearing media were selected for the production of sterile larvae of greenbottle blowfly, Phaenicia (=Lucilia) sericata (Meigen) which is widely used in maggot therapy. Eight media available in the market were used in this study. Egg hatchability was not different among the media. Survivorship of the larvae was higher in blood agar (BA), sabouraud dextrose agar, and brucella blood agar than the others. A higher content (20${\sim}$40%) of sheep blood in BA and chocolate agar increased the survivorship of larvae. The eggs and the early 3rd larvae could be stored for 12 days at $8^{\circ}C$ and for 15 days at $6^{\circ}C$ without decrease in hatchability and larval survivorship, respectively.

구더기 치료에 널리 이용되고 있는 무균 구리금파리 [Phaenicia (=Lucilia) sericata (Meigen)]를 보다 손쉽게 사육하기 위한 배지를 선발하고, 알과 유충의 저장조건을 실험하였다. 시판되고 있는 8가지의 미생물 배양용 선택배지에서 실험한 결과, 알의 부화율은 배지 간에 차이가 없었고, 유충의 생존율은 1, 2차 실험결과 blood agar(BA), sabouraud dextrose agar 및 brucella blood agar 배지에서 가장 높았다. BA 및 chocolate agar 배지에서 양의 혈액 함량을 20${\sim}$40%로 높일수록 유충의 생육이 좋았다. 알은 부화율의 저하 없이 $8^{\circ}C$에서는 12일까지 저장할 수 있었고, 3령 초기 유충은 생존율의 저하 없이 $6^{\circ}C$에서 15일까지 저장할 수 있었다.

Keywords

References

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