한국응용곤충학회지 (Korean journal of applied entomology)

  • 제48권2호
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  • Pages.211-219
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  • 2009
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  • 1225-0171(pISSN)
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  • 2287-545X(eISSN)
한국응용곤충학회 (Korean Society of Applied Entomology)
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성페로몬과 RAPD 분자지표를 이용한 사과 재배지 복숭아순나방(Grapholita molesta)발생 모니터링과 집단 이동 분석

Analysis of Migration of the Oriental Fruit Moth, Grapholita molesta, in Apple-Cultivating Areas Based on Population Monitoring Using Sex Pheromone and RAPD Molecular Marker

  • 김용균 (안동대학교 생명자원과학부) ;
  • 배성우 (국립농업과학원 산업곤충과) ;
  • 손예림 (안동대학교 생명자원과학부) ;
  • 박정아 (안동대학교 생명자원과학부)
  • Kim, Yong-Gyun (Department of Bioresource Sciences, Andong National University) ;
  • Bae, Sung-Woo (Department of Industrial Entomology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Son, Ye-Rim (Department of Bioresource Sciences, Andong National University) ;
  • Park, Jung-A (Department of Bioresource Sciences, Andong National University)
  • 발행 : 2009.06.30
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초록

사과원에 발생하는 복숭아순나방(Grapholita molesta)의 지역 및 계절적 집단 변동 상황을 성페로몬 트랩과 RAPD (random amplified polymorphic DNA) 분자마커를 이용하여 분석하였다. 사과 재배지에서 과원들 간에 이 해충의 이동을 검출하기 위해 페로몬 트랩을 사과원 내부와 이들 사과원 사이 지역에 설치하였다. 사과원 안팎에서 조사된 성페로몬 모니터링 결과는 모두 복숭아순나방의 연중(4월${\sim}$10월) 4회 성충 발생 피크를 나타냈다. 월동세대 이후 모니터링 결과를 살펴보면 사과원 외부에 비해 사과원 내부 성충 발생은 빈번한 살충제 처리에 따라 현격하게 줄었다. 사과원 내부에서 복숭아순나방은 국부적 선호성에 따른 불균등한 발생 분포를 나타냈다. RAPD 분자지표를 이용한 유전분석이 이러한 복숭아순나방의 지역 및 계절적 이동을 뒷받침하였다. 본 연구는 성페로몬을 이용한 인근 과수원 안팎의 모니터링 자료와 계절의 진행됨에 따라 집단간 유전적 분화가 감소하는 결과를 통해 사과재배지 과원들 사이에 복숭아순나방의 이동이 가능하다는 것을 제시하고 있다.

Local and seasonal populations of the oriental fruit moth, Grapholita molesta, were monitored with sex pheromone trapping and RAPD (random amplified polymorphic DNA) molecular marker to analyze their movement in apple orchards. To detect their movements among farms, pheromone traps were placed at regions between apple farms ('outside-farms') as well as within-farms ('inside-farms'). Four seasonal adult peaks were evident in apple-cultivating fields from April to October in both trappings of inside- or outside-farms. After overwintering generation, populations of inside-farms were significantly reduced with frequent insecticide applications, compared to populations of outside-farms. Within apple farms, G. molesta tended to be unevenly distributed because of significant sublocal preference. Active movements of local and seasonal populations of G. molesta were supported by gene flow analysis using RAPD marker. Monitoring data using sex pheromone and seasonal reduction in initial genetic differentiation detected in the overwintering populations suggest that there must be significant movement of G. molesta among different orchards in apple-cultivating areas.

키워드

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