International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
권호 표지

Effects of Baicalin on the differentiation and activity of preosteoclasts

  • Ko, Seon-Yle (Department of Oral Biochemistry, School of Dentistry, Dankook University)
  • Published : 2009.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Baicalin is a flavonoid purified from the medicinal plant Scutellaria baicalensis. It has been reported that baicalin exhibits antibacterial, anti-inflammatory and analgesic effects. The present study was undertaken to determine the underlying cellular mechanisms of baicalin action in preosteoclasts. The effects of this flavonoid on preosteoclasts were determined by measuring osteoclast generation and osteoclast activity in macrophage-colony stimulating factor (M-CSF)-dependent bone marrow cells (MDBMCs) and in co-cultures of MDBMCs and osteoblasts. Osteoclast generation was assayed by measuring the number of tartrateresistant acid phosphatase (TRAP) (+) multinucleated cells after culture. Osteoclast activity was assayed by measuring the area of the resorption pit after culture. We found that osteoclast generation was induced by M-CSF and receptor activator of NF-kB ligand (RANKL), and by the 1.25-dihydroxycholecalciferol in our cultures. Baicalin decreased both osteoclast generation and activity in MDBM cultures and co-cultures indicating that it may inhibit bone resorption.

Keywords

References

  1. Cai X, Li C, Du G, Cao Z. Protective effects of baicalin on ligature-induced periodontitis in rats. J Periodontal Res. 2008a;43:14-21
  2. Cai X, Li CZ, Cao ZG, Du GF, Liu LH. Protective effect of baicalin on experimental periodontitis in rats and its possible mechanisms. Zhonghua Kou Qiang Yi Xue Za Zhi. 2008b;43:281-5
  3. Chen KM, Ge BF, Ma HP, Zheng RL. The serum of rats administered flavonoid extract from Epimedium sagittatum but not the extract itself enhances the development of rat calvarial osteoblast-like cells in vitro. Pharmazie. 2004;59:61-4
  4. Do SH, Lee JW, Jeong WI, Chung JY, Park SJ, Hong IH, Jeon SK, Lee IS, Jeong KS. Bone-protecting effect of Rubus coreanus by dual regulation of osteoblasts and osteoclasts. Menopause. 2008;15:676-83 https://doi.org/10.1097/gme.0b013e31815bb687
  5. Gao Z, Huang K, Yang X, Xu H. Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi. Biochim Biophys Acta. 1999;1472:643-50 https://doi.org/10.1016/S0304-4165(99)00152-X
  6. Ha H, Kwak HB, Lee SW, Jin HM, Kim HM, Kim HH, Lee Z.H. Reactive oxygen species mediate RANK signaling in osteoclasts. Exp Cell Res. 2004;301:119-27 https://doi.org/10.1016/j.yexcr.2004.07.035
  7. Hidaka S, Okamoto Y, Nakajima K. Preventive effects of traditional Chinese medicines on experimental osteoporosis induced by ovariectomy in rats. Caicif Tissue Int. 1997;61:239-46 https://doi.org/10.1007/s002239900329
  8. Kim JK, Kim SW, Kim HY, Lee BE, Ko SY. OPB, a water extract from Rehmannia glutinosa Libosch and Eleutherococcus senticosus Mas, inhibits osteoclast differentiation and function. Int J Oral Biol. 2007;32:23-34
  9. Ko SY. Effects of Baicalin on the Proliferation and Activity of osteoblastic cells. Korean J Oral Medicine 2008;33:105-10
  10. Liu WC, Li XW, Li YS. Cai KY, Yao KD, Yang Z, Li X. Effects of baicalin-modified poly (D,L-lactic acid) surface on the behavior of osteoblasts. J Mater Sci Mater Med 2003;14:961-5 https://doi.org/10.1023/A:1026398532009
  11. Minkin C. Bone acid phosphatase: Tartrate-resistant acid phosphatase as a marker of osteoclast function. Calcif Tissue Int. 1982;34:285-90 https://doi.org/10.1007/BF02411252
  12. Ono Y, Fukaya Y, Imai S, Yamakuni T. Beneficial effects of Ajuga decumbens on osteoporosis and arthritis. Biol Pharm Bull. 2008;31:1199-204 https://doi.org/10.1248/bpb.31.1199
  13. Reddy SV. Regulatory mechanisms operative in osteoclasts. Crit Rev Eukaryot Gene Expr. 2004;14:255-70 https://doi.org/10.1615/CritRevEukaryotGeneExpr.v14.i4.20
  14. Reginster JY. Treatment of bone in elderly subjects: calcium, vitamin D, fluor, bisphosphonates, calcitonin. Horm Res. 1995;43:83-8 https://doi.org/10.1159/000184243
  15. Rodan GA. Emerging therapies in osteoporosis. Ann Rep Med Chem. 1994;29:275-85 https://doi.org/10.1016/S0065-7743(08)60741-1
  16. Sasaki T, Takahashi N, Higashi S, Suda T. Multinucleated cells formed on calcified dentin from mouse bone marrow cells treated with $1\alpha$,25-dihydroxyvitamin $D_{3}$ have ruffled borders and resorb dentin. Anat Rec. 1989;224:379-91 https://doi.org/10.1002/ar.1092240307
  17. Shen YC, Chiou WF, Chou YC, Chen CF. Mechanisms in mediating the anti-inflammatory effects of baicalin and baicalein in human leukocytes. Eur J Pharmacol. 2003;465:171-81 https://doi.org/10.1016/S0014-2999(03)01378-5
  18. Song YZ, Yang YZ, Jia YZ. Antagonistic effect of baicalin on oxidative stress injury in neurons and astrocytes of rats. Zhongguo Zhong Xi Yi Jie He Za Zhi 2004;24:339-42
  19. Stock JL. Drug therapy in Osteoporosis, in Diagnostic and Therapeutic Principles. edited by Rosen CJ, pp 173-187, Humana Press. Totowa, NJ, 1996
  20. Suda T, Takahashi N, Udagawa N, Jimi E, Gillespie MT, Martin TJ. Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families. Endocr Rev. 1999;20:345-57 https://doi.org/10.1210/er.20.3.345
  21. Takahashi N, Yamana H, Yoshiki S, Roodman GD, Mundy GR, Jones SJ, Boyde A, Suda T. Osteoclast-like cell formation and its regulation by osteotropic hormones in mouse bone marrow cultures. Endocrinol. 1988a;122:1373-82 https://doi.org/10.1210/endo-122-4-1373
  22. Takahashi N, Akastu T, Sasaki T, Nicholson GC, Moseley JM, Martin TJ, Suda T. Induction of calcitonin receptors by $1\alpha$,25-dihydroxyvitamin $D_{3}$ in osteoclast-like multinucleated cells formed from mouse bone marrow cells. Endocrinol. 1988b;123:1504-10 https://doi.org/10.1210/endo-123-3-1504
  23. Wang GF, Wu ZF, Wan L, Wang QT, Chen FM. Influence of baicalin on the expression of receptor activator of nuclear factor-kappaB ligand in cultured human periodontal ligament cells. Pharmacology. 2006;77:71-7 https://doi.org/10.1159/000092853
  24. Wasnich R. What is an osteoporotic fracture? in Osteoporosis, Diagnotic and Therapeutic Principles, edited by Rosen CJ, pp 79-88, Humana Press. Totowa, NJ, 1996
  25. Xie F, Wu CF, Lai WP, Yang XJ, Cheung PY, Yao XS, Leung PC, Wong MS. The osteoprotective effect of Herba epimedii(HEP) extract in vivo and in vitro. eCAM. 2005;2:353-61
  26. Yeung HC. Handbook of Chinese herbs and formulas. Institute of Chinese medicine, Los Angeles, 1985
  27. Zhang DW, Cheng Y, Wang NL, Zhang JC, Yang MS, Yao XS. Effects of total flavonoids and flavonol glycosides from Epimedium koreanum Nakai on the proliferation and differentiation of primary osteoblasts. Phytomedicine. 2008;15:55-61 https://doi.org/10.1016/j.phymed.2007.04.002