Journal of Pharmaceutical Investigation

The Korean Society of Pharmaceutical Sciences and Technology (한국약제학회)
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Preliminary Imaging Analysis for Enhanced Intestinal Uptake of Non-soluble Polystyrene Microspheres in the Presence of Oleic Acid using Rat Intestine

  • Tran, Huyen Thi Thanh (Bioavailability Control Laboratory, College of Pharmacy, Kangwon National University) ;
  • Tran, Phuong Ha Lien (Bioavailability Control Laboratory, College of Pharmacy, Kangwon National University) ;
  • Tran, Thao Truong-Dinh (Bioavailability Control Laboratory, College of Pharmacy, Kangwon National University) ;
  • Lee, Kyung-Ho (Bioavailability Control Laboratory, College of Pharmacy, Kangwon National University) ;
  • Lee, Beom-Jin (Bioavailability Control Laboratory, College of Pharmacy, Kangwon National University)
  • Published : 2009.06.20
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Abstract

In vitro intestinal uptake of non-soluble polystyrene microspheres (NPMS) was visualized with and without oleic acid using a fluorescence microscopy. Fluorescent polystyrene latex microspheres with 1${\mu}$m larger size were used as models for nonspecifically absorbed nonbiodegradable particulates. The NPMS could not penetrate the enterocytes but a few NPMS could be penetrated via Peyer's patches. When the oleic acid was mixed with NPMS, the transporting efficiency of NPMS through enterocytes as well as Peyer's patches was significantly enhanced. The modification of the intestinal membrane permeability and surface feature of the NPMS in the presence of oleic acid might be a clue to the transport of NSPM although the detailed mechanism is still under investigation.

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References

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