Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Detection of influenza A viruses by RT-PCR with single primer of nonstructural gene

비구조 단백질 유전자 primer를 사용한 RT-PCR에 의한 인플루엔자 A형 바이러스의 검출

  • Moon, Hyeong-Sun (School of Veterinary Medicine, Kangwon National University) ;
  • Bae, Yoon-Yeong (School of Veterinary Medicine, Kangwon National University) ;
  • Jin, Ji-Dong (School of Veterinary Medicine, Kangwon National University) ;
  • Kang, Zheng-Wu (School of Veterinary Medicine, Kangwon National University) ;
  • Hahn, Tae-Wook (School of Veterinary Medicine, Kangwon National University)
  • 문형선 (강원대학교 수의학부(대학)) ;
  • 배윤영 (강원대학교 수의학부(대학)) ;
  • 김길동 (강원대학교 수의학부(대학)) ;
  • 강정무 (강원대학교 수의학부(대학)) ;
  • 한태욱 (강원대학교 수의학부(대학))
  • Published : 2009.06.30
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Abstract

Influeza type A virus have been worldwide problematic in animals as well as in humans. In this study, the use of reverse-transcriptase polymerase chain reaction (RT-PCR) was described for detecting influenza virus type A. The primer of RT-PCR was designed from an nonstructural (NS) gene of Influenza A virus. By RT-PCR, a product with the size of 189 bp was detected only when influenza virus type A was used as template. No products could be detected with Influenza virus type B as well as other respiratory pathogens. The detection limit of the RT-PCR was up to $10^{0.3}TCID_{50}$ which is comparable to the sensitivity of cell culture method. The RT-PCR could detect the influenza A virus from nasal turbinates of the ferrets infected with influenza virus type A not type B.

Keywords

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