Molecular & Cellular Toxicology

The Korean Society of Toxicogenomics and Toxicoproteomics (대한독성유전 단백체학회)
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Alaria esculenta Extract Protects against Oxidative Damage by Inducing Heme Oxygenase-1 Expression via Akt and Nrf2

  • Choi, Chun-Yeon (Department of Biochemistry, College of Medicine, Inje University) ;
  • Jo, Guk-Heui (Department of Biochemistry, College of Medicine, Inje University) ;
  • Lee, Jung-Im (Division of Marine Environment and Bioscience, Korea Maritime University) ;
  • Seo, Young-Wan (Division of Marine Environment and Bioscience, Korea Maritime University) ;
  • Han, Tae-Jun (Department of Biology, University of Incheon) ;
  • Choi, Il-Whan (Department of Microbiology, College of Medicine, Inje University) ;
  • Liu, Kwang-Hyeon (Department of Pharmacology, College of Medicine, Inje University) ;
  • Oh, Sang-Taek (Department of Pharmacology, College of Medicine, Inje University) ;
  • Kim, Dong-Eun (Division of Bioscience and Biotechnology, Konkuk University) ;
  • Jang, Won-Hee (Department of Biochemistry, College of Medicine, Inje University) ;
  • Seog, Dae-Hyun (Department of Biochemistry, College of Medicine, Inje University) ;
  • Park, Yeong-Hong (Department of Biochemistry, College of Medicine, Inje University) ;
  • Yea, Sung-Su (Department of Biochemistry, College of Medicine, Inje University)
  • Published : 2009.06.30
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Abstract

Alaria esculenta is a brown seaweed found in the Arctic. This study investigated the protective effect of A. esculenta extract (AEE) against oxidant-mediated injury and its mode of action in RAW264.7 macrophages. The methyl thiazolyl tetrazolium (MTT) assay showed that $H_2O_2$ treatment reduced cell viability, whereas AEE protected cells from $H_2O_2$-mediated cytotoxicity in a dose-dependent manner. Because heme oxygenase-1 (HO-1) is known to protect cells against oxidative damage, we investigated the effect of AEE on HO-1 gene expression and HO enzyme activity. The protective effect of AEE against $H_2O_2$-induced injury was correlated with increased HO enzyme activity. AEE also induced HO-1 mRNA and protein expression, as determined RT-PCR and Western blotting, respectively. To characterize the mechanisms by which AEE induces HO-1 gene expression, we examined the effect of AEE on the nuclear translocation of NF-E2-related factor-2 (Nrf2) and Akt phosphorylation. AEE treatment activated upstream signaling for HO-1 gene expression, including the nuclear translocation of Nrf2 and Akt phosphorylation. Collectively, these results suggest that AEE has anti-oxidant activity that is mediated, at least in part, via the activation of Nrf2 and Akt and the subsequent induction of HO-1 gene expression.

Keywords

References

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