Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
권호 표지

The Effect of the Radix Ginseng on Expression of CDSI and GFAP Following Hypoxic Injury on Central Nervous System

인삼(人蔘)이 허혈성 중추신경 손상으로 발현 증가된 CD81 및 GFAP에 미치는 영향

  • Seo, Jong-Hoon (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Song, Bong-Gun (Department of Internal Medicine, Wonkwang University Oriental Medical School) ;
  • Lyu, Yeoung-Su (Dept. of Neoropsychiatry, College of Oriental Medicine, Wonkwang University) ;
  • Kang, Hyung-Won (Dept. of Neoropsychiatry, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Tae-Heon (Dept. of Neoropsychiatry, College of Oriental Medicine, Wonkwang University)
  • 서중훈 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 송봉근 (원광대학교 한의과대학 내과학교실) ;
  • 류영수 (원광대학교 한의과대학 신경정신과 교실) ;
  • 강형원 (원광대학교 한의과대학 신경정신과 교실) ;
  • 김태헌 (원광대학교 한의과대학 신경정신과 교실)
  • Published : 2009.03.30
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Abstract

Objectives : Gliosis disturbs recovery of damaged astrocytes following central nervous system(CNS) injury. Gliosis relates to up-regulation of CD81 and GFAP. In glial cells at injured CNS, the expression of CD81 and GFAP is increased. It is possible that when the gliosis formation is suppressed, regeneration of oxons can occur. According to the recent study, the treatment with anti CD81 antibodies enhanced functional recovery in rats with spinal injury. So, the author studies the effect of water extract of Radix Ginseng on regulation of CD81 and GFAP with CNS injury. Methods : In the cell study, hypoxic damage was induced by CoC12. And according to Longa et al, cerebral ischemia was made by middle cerebral artery occlusion in the rat. Cross sections of rat brain were examined under microscope. MTT analysis was performed to examine cell viability, cell based ELISA, Western Blot and PCR were used to detect the expression of CD81 and GFAP. Results : The following results were obtained. 1. We found that CD81 and GFAP were decreased in hypoxic injured cells following Radix Ginseng administration. 2. We injected the extract of Radix Ginseng to the middle cerebral artery occlusion in rats, and the immunohistochemistry analysis showed that CDS1 and GFAP were decreased. Conclusions : These results show that the extract of Radix Ginseng could suppress the gliosis formation and prevent cell death, by controlling the expression of CDS1 and GFAP. Therefore, Radix Ginseng could be a useful to regenerate CNS injury.

Keywords

References

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