The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effects of Spatholobi Caulis MeOH Extract on the Production of NO and Pro-inflammatory Cytokines in LPS-activated Raw264.7 Cells

계혈등 메탄올추출물이 LPS로 활성화된 Raw264.7 Cell에서 Nitric Oxide 및 Pro-inflammatory Cytokines 생성에 미치는 영향

  • Choe, Song-I (Korea Herbal Cosmeceutical Material Bank, Daegu Haany University) ;
  • Park, Sook-Jahr (College of Oriental Medicine, Daegu Haany University, BK21, Daegu Haany University) ;
  • Byun, Sung-HUi (College of Oriental Medicine, Daegu Haany University) ;
  • Lee, Jong-Rok (College of Oriental Medicine, Daegu Haany University, Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University) ;
  • Park, Moon-Ki (Korea Herbal Cosmeceutical Material Bank, Daegu Haany University) ;
  • Kim, Sang-Chan (College of Oriental Medicine, Daegu Haany University, BK21, Daegu Haany University)
  • 최송이 (대구한의대학교 국가지정 한약자원 향장소재은행) ;
  • 박숙자 (대구한의대학교 한의과대학, 대구한의대학교 BK21) ;
  • 변성희 (대구한의대학교 한의과대학) ;
  • 이종록 (대구한의대학교 한의과대학, 대구한의대학교 한방생명자원연구센터) ;
  • 박문기 (대구한의대학교 국가지정 한약자원 향장소재은행) ;
  • 김상찬 (대구한의대학교 한의과대학, 대구한의대학교 BK21)
  • Published : 2009.06.30
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Abstract

Objectives: Spatholobi Caulis is the stem of Spatholobus suberectus Dunn., which has been used for treating menstrual disorders, anemia, and rheumatic arthralgia in traditional Oriental medicine, This study was conducted to evaluate the inhibitory effects of Spatholobi Caulis extract (SCE) on the production of nitric oxide (NO) and pro-inflammatory cytokines in LPS-activated Raw264,7 cells. Methods : Cell viability was determined by MIT assay. Relative levels of NO were measured with Griess reagent and pro-inflammatory cytokines were detected by ELISA Expression of iNOS and COX-2 proteins were determined by western blotting. Results : NO production and iNOS expression were increased by incubation with LPS for 24 h. However, the augmented NO was reduced by SCE in a dose-dependent manner. Expressions of iNOS and COX-2 were inhibited by the treatment with SCE, SCE also suppressed the production of pro-inflammatory cytokines, such as IL-1beta and IL-6. Conclusions : These results indicate that the MeOH extract of Spatholobi Caulis warrant further development as an anti-inflammatory agent for the treatment of Gram-negative bacterial infections.

Keywords

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