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Morphological and Physiological Characteristics of Amanita hemibapha subsp. hemibapha (Berk. & Broome) Sacc.

달걀버섯의 형태 및 생리적 특성

  • Sou, Hong-Duck (Department of Forestry, College of Agricultural Life Science, Kyungpook National University) ;
  • Hur, Tae-Chul (Department of Forestry, College of Agricultural Life Science, Kyungpook National University) ;
  • Joo, Sung-Hyun (Department of Forestry, College of Agricultural Life Science, Kyungpook National University)
  • 서홍덕 (경북대학교 농업생명과학대학 임학과) ;
  • 허태철 (경북대학교 농업생명과학대학 임학과) ;
  • 주성현 (경북대학교 농업생명과학대학 임학과)
  • Published : 2009.06.30

Abstract

This study was conducted to investigate optimum cultural conditions for Amanita hemibapha isolated in Korea and its hyphae characteristics. Micrographs shows the presence of clamp connection. A. hemibapha grows as mycelial form(M-phase) 2-4 ${\mu}m$ and yeast-like form(Y-phase) 7-8 ${\mu}m$. The fungal spores were broadly elliptical and papillate, 8-11 ${\times}$ 6-9 ${\mu}m$ in size. The nucleotide sequence analysis of the ITS of nuclear ribosomal DNA from sporocarps and in-vitro-grown mycelium supported the fungal species is Amanita hemibapha. A. hemibapha showed sequence similarity in the ITS rDNA with A. caesarea(97.5) and A. jacksonii(98.5%) which are morphologically similar species to A. hemibapha. The optimal pH and temperature for mycelial growth of A. hemibapha were pH 6.0 and $28^{\circ}C$, respectively. The fungal species showed best growth in SYP and GYS medium. A. hemibapha grew well with mannitol and glucose as carbon sources and peptone as a nitrogen source.

달걀버섯 균사의 특징 및 동정과 균사 생육을 위한 최적배지 조건을 조사하였다. 실체, 광학, 주사전자현미경을 통하여 달걀버섯의 균사를 관찰하였다. 그 결과, clamp connection을 볼 수 있었으며, 균사형(M형)직경이 2-4 ${\mu}m$, 효모형(Y형)균사 직경이 7-8 ${\mu}m$로 나타났다. 포자는 광타원형-원형으로 $8-11\;{\times}6-9$ ${\mu}m$로 관찰되었다. 채집한 달걀버섯을 통해 분자학적으로 동정한 결과, A. hemibapha는 A. caesarea와 A. jacksonii와 각각 97.5, 98.5%의 높은 유사성을 나타내었다. 균사생육을 위한 최적 온도는 $28^{\circ}C$이고, pH6.0에서 최적 균사생육을 나타내었다. 또한, SYP, GYS 배지를 사용하였을 때 가장 우수한 균사생육을 나타내었다. 탄소원으로는 Mannitol과 Glucose를 첨가 하였을 때 균사 생육이 가장 활발히 나타났고, 질소원으로는 Peptone이 첨가 되었을 때 가장 좋은 생육이 이루어졌다.

Keywords

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Cited by

  1. Analysis of Environmental Characteristics in Habitat of Amanita hemibapha vol.39, pp.3, 2011, https://doi.org/10.4489/KJM.2010.39.3.164
  2. Nitrogen Source-requirement and Preference of Ectomycorrhizal Fungi in Pure Culture vol.41, pp.3, 2013, https://doi.org/10.4489/KJM.2013.41.3.149