한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
권호 표지

반응표면분석에 의한 사과 pomace로부터의 폴리페놀 추출조건 최적화

Optimization of Extraction Conditions of Polyphenolic Compounds from Apple Pomace by Response Surface Methodology

  • 발행 : 2009.06.30
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초록

사과 pomace의 알콜 추출 조건을 최적화하기 위하여 중심합성계획법을 이용하여 용매비와 침지시간, 추출시간, 추출온도는 독립변수로 수율과 총 폴리페놀 함량은 종속변수로 설정하였다. 실험 결과 최적조건은 반응표면분석을 이용하여 모니터링하였다. 반응표면그래프 분석 결과 사과 pomace의 알콜 추출 시 수율은 침지시간과 추출시간에는 큰 영향을 받지 않았으며, 시료에 대한 용매비가 높을수록 또는 추출온도가 높아질수록 증가하였다. 총폴리페놀 함량은 모든 독립변수에 영향을 받아 비례적으로 함께 증가하였으나 최고점을 보인 후 다시 감소되는 경향을 나타내었다. 총 폴리페놀 함량을 최대로 하는 최적 알콜 추출 조건은 사과 pomace에 용매비를 13.00 mL/g로 하고 89.02분 동안 침지시킨 후 $70^{\circ}C$에서 180분 추출하였을 때로서 그 때의 수율은 3.76%, 총 폴리페놀 함량은 29.68 mg catechin equiv./g으로 나타났다.

This study examined the optimization of alcohol extraction conditions for maximizing the total polyphenols derived from apple pomace, by response surface methodology (RSM). The effects of four independent variables, including $X_1$ (ratio of solvent to sample content), $X_2$ (dipping time), $X_3$ (extraction time), and $X_4$(extraction temperature), were investigated at five levels using central composite design (CCD). $Y_1$ (yield) and $Y_2$ (total polyphenols) were chosen as dependent variables. The coefficients of determination, $R^2$, were greater than 0.900 (0.9042 and 0.9555). The results showed that the model fit was very significant (p<0.001). The optimum extraction conditions were as follows: 13.00 mL/g for the ratio of solvent to sample content, 89.02 min for dipping time, 180 min for extraction time, and $70^{\circ}C$ for extraction temperature. At these conditions, the predicted total polyphenol content was 29.68 mg catechin equiv./g.

키워드

참고문헌

  1. Whang HJ. Changes of phenolic compounds in Korean apple (fuji) during maturation. Korean J. Food Nutr. 12: 364-369 (1999)
  2. Yun HJ, Lim SY, Hur JM, Jeong JW, Yang SH, Kim DH. Changes of functional compounds in, and testure characteristics of, apples, during post-irradiation storage at different temperatures. Korean J. Food Preserv. 14: 239-246 (2007)
  3. Agricultural & Forestry Statistical Yearbook, Ministry of Agriculture & Forestry, Seoul, Korea. pp. 116-117 (2006)
  4. Kim SD, Jang KS, Kim MK. Fermentation of apple vinegar in the farmhouse. J. East Asian Soc. Diet. Life 4: 79-90 (1994)
  5. Sudha ML, Baskaran V, Leelavathi K. Apple pomace as a source of dietary fiber and polyphenols and its effect on the rheological characteristics and cake making. Food Chem. 104: 686-692 (2007) https://doi.org/10.1016/j.foodchem.2006.12.016
  6. Sohn KS, Seog EJ, Lee JH. Quality characteristics of clarified apple juices produced by various methods. Korean J. Food Preserv. 13: 138-143 (2006)
  7. Lu Y, Foo LY. Antioxidant and radical scavenging activities of polyphenols from apple pomace. Food Chem. 68: 81-85 (2000) https://doi.org/10.1016/S0308-8146(99)00167-3
  8. Cha JY, Kim HJ, Chung CH, Cho YS. Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata. J. Korean Soc. Food Sci. Nutr. 28: 1310-1315 (1999)
  9. Hong JJ, Ahn TH. Changes in total flavonoid and total polyphenol contents of leafy vegetables (spinach, chard and whorled mallow) by blanching time. Korean J. Food Cook. Sci. 21: 190-194 (2005)
  10. An BJ, Park TS, Lee JY, Park GH, Hyun SJ, Lee JT, Cho YJ, Kim YS. Growth inhibitory effect of irradiated green tea polyphenol addition in cosmetic composition. J. Korean Soc. Appl. Biol. Chem. 50: 217-223 (2007)
  11. Whang HJ, Han WS, Yoon KR. Quantitative analysis of total phenolic content in apple. Anal. Sci. Technol. 14: 377-383 (2001)
  12. Lu Y, Foo LY. Identification and quantification of major polyphenols in apple pomace. Food Chem. 59: 187-194 (1997) https://doi.org/10.1016/S0308-8146(96)00287-7
  13. Renard CMGC, Dupont N, Guillermin P. Concentrations and characteristics of procyanidins and other phenolics in apples during fruit growth. Phytochemistry 68: 1128-1138 (2007) https://doi.org/10.1016/j.phytochem.2007.02.012
  14. Jang DH. Maple Use for RSM education. Korea Satistical Society, Seoul, Korea (2005)
  15. Lee MS, Ryu GH. Optimization of hot water extraction conditions for cod byproduct by response surface methodology analysis. Food Eng. Prog. 10: 248-255 (2006)
  16. Kim HK, Do JR, Hong JH, Lee GD. Optimization of extraction conditions for cabbage. J. Korean Soc. Food Sci. Nutr. 34: 1625-1632 (2005) https://doi.org/10.3746/jkfn.2005.34.10.1625
  17. Kim JO, Kwon ST, Lee GD, Hong JH, Moon DH, Kim TW, Kim DI. Optimization of extraction condition on fig (Ficus carica L.) by response surface methodology. Korean J. Food Preserv. 15: 66-73 (2008)
  18. Singleton V, Rossi J. Colorimetry of total phenolics with phosphomolybdic- phosphotungstic acid reagents. Am. J. Enol. Viticult. 16: 144-158 (1965)
  19. Kim HK, Do JR, Hong JH, Lee GD. Optimization for functional properties of cabbage extracts. Korean J. Food Preserv. 12: 591-599 (2005)
  20. Park NY, Kwon JH, Kim HK. Optimization of extraction conditions for ethanol extracts from chrysanthemum morifolium by response surface methodology. Korean J. Food Sci. Technol. 30: 1189-1196 (1998)
  21. Kim NM, Sung HS, Kim WJ. Effect of solvents and some extraction conditions on antioxidant activity in cinnamon extracts. Korean J. Food Sci. Technol. 25: 204-209 (1993)
  22. Choi MA, Park NY, Woo SM, Jeong YJ. Optimization of extraction conditions from Hericium erinaceus by response surface methodology. Korean J. Food Sci. Technol. 35: 777-782 (2003)
  23. Abad-Garcia B, Berrueta LA, Lopez-Marquez DM, Crespo-Ferrer I, Gallo B, Vicente F. Optimization and validation of a methodology based on solvent extraction and liquid chromatography for the simultaneous determination of several polyphenolic families in fruit juices. J. Chromatogr. 1154: 87-96 (2007) https://doi.org/10.1016/j.chroma.2007.03.023
  24. Adil IH, Cetin HI, Yener ME, Bayindirli A. Subscritical (carbon dioxide+ethanol) extraction of polyphenols from apple and peach pomaces, and determination of the antioxidant activities of the extracts. J. Supercrit. Fluid. 43: 55-63 (2007) https://doi.org/10.1016/j.supflu.2007.04.012