Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Availability of Identification by RAPD of Aspergillus species from Sputum

객담에서 분리한 Aspergillus 속의 RAPD를 이용한 분자생물학적 동정의 유용성

  • Kim, Young-Kwon (Korean Collection of Medical Fungi, Medical Science College of Konyang University) ;
  • Hong, Sung-Rho (Department of Clinical Laboratory Science, Dongnam Health University) ;
  • Kim, Sang-Ha (Department of Public Health and Welfare, Graduate School of Konyang University) ;
  • Seo, Choong-Wonand (Department of Laboratory Medicine, Konyang University Hospital)
  • 김영권 (건양대학교 의과학대학 한국의진균자원센터) ;
  • 홍성노 (동남보건대학 임상병리과) ;
  • 김상하 (건양대학교 보건복지대학원) ;
  • 서충원 (건양대학교병원 진단검사의학과)
  • Received : 2009.11.16
  • Accepted : 2009.12.16
  • Published : 2009.12.31
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Abstract

On the basis of morphological characteristics, of total 128 strains of from sputum of tuberculos inpatient were identified as A. fumigatus (61 strains), A. niger (37), A. flavus (26), A. versicolor (1), A. nidulans (1), A. clavatus (1) and Neosartorya fennelliae (1). These strains were re-identified according to recent Aspergillus classification system which is mainly based on molecular characteristics. The strains were grouped by randomly amplified polymorphic DNA (RAPD) techniques. The representative strains from each group were sequenced with partial ${\beta}$-tubulin gene and compared with those of reference strains in the Aspergillus and were identified by the sequence. The identification was confirmed by morphology examination. As the results, they are reidentified as A. fumigatus (58), A. niger (11), A. tubingensis (26), A. flavus (27), A. sydowii (3), A. nidulans (1), A. clavatus (1) and Neosatorya fennelliae (1). This is the first report of A. tubuingensis in clinical field in Korea.

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