Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)
- Volume 41 Issue 4
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- Pages.158-166
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- 2009
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- 1738-3544(pISSN)
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- 2288-1662(eISSN)
Availability of Identification by RAPD of Aspergillus species from Sputum
객담에서 분리한 Aspergillus 속의 RAPD를 이용한 분자생물학적 동정의 유용성
- Kim, Young-Kwon (Korean Collection of Medical Fungi, Medical Science College of Konyang University) ;
- Hong, Sung-Rho (Department of Clinical Laboratory Science, Dongnam Health University) ;
- Kim, Sang-Ha (Department of Public Health and Welfare, Graduate School of Konyang University) ;
- Seo, Choong-Wonand (Department of Laboratory Medicine, Konyang University Hospital)
- Received : 2009.11.16
- Accepted : 2009.12.16
- Published : 2009.12.31
Abstract
On the basis of morphological characteristics, of total 128 strains of from sputum of tuberculos inpatient were identified as A. fumigatus (61 strains), A. niger (37), A. flavus (26), A. versicolor (1), A. nidulans (1), A. clavatus (1) and Neosartorya fennelliae (1). These strains were re-identified according to recent Aspergillus classification system which is mainly based on molecular characteristics. The strains were grouped by randomly amplified polymorphic DNA (RAPD) techniques. The representative strains from each group were sequenced with partial