Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Forensic Evaluation of Prostate-Specific-Antigen (PSA) Rapid Test Kit for Identification of Human Semen

전립선특이항원검사 Kit에 의한 정액의 신속 검출법

  • Lim, Chae-Won (Department of Scientific Investigation Section, Daegu Metropolitan Police Agency) ;
  • Lee, Jong-Hoon (Department of Scientific Investigation Section, Daegu Metropolitan Police Agency) ;
  • Kim, Hyung-Lak (Department of Laboratory medicine, Sunchon St. Garollo Hospital)
  • 임채원 (대구지방경찰청 과학수사계) ;
  • 이종훈 (대구지방경찰청 과학수사계) ;
  • 김형락 (순천성가롤로병원 진단검사의학과)
  • Received : 2009.04.06
  • Accepted : 2009.06.25
  • Published : 2009.06.30
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Abstract

It would be one of the most important tests that determination of semen in crime scene as a matter of significant evidences. Recently, it has been developed for the identification of semen in forensic specimens which was used simply, easily and reproductively. In this study, Prostate-Specific-Antigen (PSA) Rapid Test kit was evaluated for the forensic identification of semen and compared with one step semen inspection forensic rapid test kit. The sensitivity and specificity of the rapid PSA kit were examined in addition to the stability of PSA. The positive band of rapid PSA kit shown even with 1,000,000-fold diluted semen, which was at least 100 timed higher than qualitative one step semen inspection forensic rapid test kit. PSA was detected in urine from normal male adult, however, it was not detected in urine from young boys and female body fluids. It was shown that PSA was very stable to resist boiling for 20 minutes and the effect of bacteria. In crime scene investigation, rapid PSA kit is expected to help to identify semen easily in the evidences.

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