Journal of the Korean Magnetic Resonance Society (한국자기공명학회논문지)

Korean Magnetic Resonance Society (한국자기공명학회)
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Sensitivity Enhancement of Methyl-TROSY by Longitudinal 1H Relaxation Optimization

  • Lee, Dong-Han (Department of NMR-based Structural Biology, Max Planck Institute for Biophysical Chenistry) ;
  • Vijayan, Vinesh (Department of NMR-based Structural Biology, Max Planck Institute for Biophysical Chenistry) ;
  • Montaville, Pierre (Department of NMR-based Structural Biology, Max Planck Institute for Biophysical Chenistry) ;
  • Becker, Stefan (Department of NMR-based Structural Biology, Max Planck Institute for Biophysical Chenistry) ;
  • Griesinger, Christian (Department of NMR-based Structural Biology, Max Planck Institute for Biophysical Chenistry)
  • Published : 2009.06.20
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Abstract

The NMR detection of methyl groups is of keen interest because they provide the long-range distance information required to establish global folds of high molecular weight proteins. Using longitudinal $^1H$ relaxation optimization, we achieve a gain in sensitivity of approximately 1.6-fold in the methyl-TROSY and its NOESY experiments for the 38 kDa protein mitogen activated protein kinase p38 in its fully protonated and $^{13}C$ and $^{15}N$ labeled state.

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References

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