Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Evaluation of Estrogenic Activity of Pumpkin Seed Extract using Recombinant Yeast Assay

재조합 효모를 이용한 호박씨 추출물의 에스트로겐 활성 평가

  • Tahk, Hong-Min (Department of Food and Nutrition, College of Human Ecology, Chung-Ang University) ;
  • Lee, Bog-Hieu (Department of Food and Nutrition, College of Human Ecology, Chung-Ang University) ;
  • Rho, Sook-Nyung (Department of Food and Nutrition, College of Human Ecology, Chung-Ang University) ;
  • Kim, Chun-Soo (McCoy Corporation) ;
  • Jung, Ji-Youn (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Choi, Chang-Sun (Department of Food and Nutrition, College of Human Ecology, Chung-Ang University)
  • 탁홍민 (중앙대학교 생활과학대학 식품영양학과) ;
  • 이복희 (중앙대학교 생활과학대학 식품영양학과) ;
  • 노숙령 (중앙대학교 생활과학대학 식품영양학과) ;
  • 김천수 ((주)맥코이교역) ;
  • 정지윤 (공주대학교 산업과학대학 특수동물학과) ;
  • 최창순 (중앙대학교 생활과학대학 식품영양학과)
  • Published : 2009.06.30
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Abstract

The aim of this study is to evaluate the estrogenic activity of Cucurbita pepo seed extract which includes ${\beta}-sitosterol$ and other phytosterols. Sample was extracted from Cucurbita pepo seed by supercritical carbondioxide method and resuspended with ethanol. Estrogenic activity was measured by recombinant yeast assay which detects estrogenic activity using recombinant yeast with high level of estrogenic receptor. However, estrogenic activity of pumpkin seed extract was not found in this study. Based on this data, pumpkin seed extract will not cause estrogenic disturbance.

기능성 식품 소재로 주목을 받고 있는 호박종자추출물의 에스트로겐 활성을 재조합 효모를 이용하여 측정하였다. 양성대조물질로 사용된 $17{\beta}-Estradiol$은 강력한 에스트로겐 활성을 나타내었으며, 농도의존적인 반응을 나타내었다. 초임계추출법에 의하여 얻어진 호박종자추출물 분말을 에탄올로 재추출하여 시험에 사용한 결과 에스트로겐 활성이 관찰되지 않았다. 따라서 호박종자추출물이 건강기능식품 제조에 사용될 때 에스트로겐 활성으로 인한 부작용은 없을 것으로 기대된다.

Keywords

References

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