Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Effect of poly(lactide-co-glycolide) (PLGA) on bone regeneration in rabbit calvaria

토끼 두개골 결손부의 골재생에 대한 poly(lactide-co-glycolide) (PLGA) 의 영향

  • Park, Jae-Young (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Hwang, Woo-Jin (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Jeong, Seong-Nyum (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Kim, Yun-Sang (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Pi, Sung-Hee (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • You, Hyung-Keun (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Shin, Hyung-Shik (Department of Periodontology, School of Dentistry, Wonkwang University)
  • 박재영 (원광대학교 치과대학 치주과학교실) ;
  • 황우진 (원광대학교 치과대학 치주과학교실) ;
  • 정성념 (원광대학교 치과대학 치주과학교실) ;
  • 김윤상 (원광대학교 치과대학 치주과학교실) ;
  • 피성희 (원광대학교 치과대학 치주과학교실) ;
  • 유형근 (원광대학교 치과대학 치주과학교실) ;
  • 신형식 (원광대학교 치과대학 치주과학교실)
  • Published : 2009.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: The purpose of this study is to histologically and histomorphometrically evaluate the effect of PLGA on bone regeneration compared with bone graft material. Methods: The experimental study was conducted in 10 rabbits with 2 different healing periods of 2 and 4 weeks. Following surgical exposure of the calvarium, 4 circular bone defects with a diameter of 4.6mm were formed. Rabbits were divided into control group, test groups I, and II. 10 defects assigned to the test group Ⅰ were grafted with Nu-oss and other 10 defects assigned to the test group II were grafted with PLGA. The rest of the defects were in the negative control group. At 2nd and 4th week after surgery, 10 rabbits were sacrificed through intracardiac perfusion and then specimens were obtained. Histological analysis was performed following staining with trichorme and transversal sectioning of the calvarial bone. Results: A group which used PLGA showed tissue reactions characterized by severe inflammation, rather than distinctive new bone formation. Conclusions: The present experimental investigations have failed to prove any beneficial effects of PLGA. PLGA used in this study exhibited foreign body reactions and a less favorable pattern of new bone formation in comparison to control group. Conclusion: PLGA did not function as scaffold. Further investigations of many types of micro PLGA that could improve its potential in GBR procedures are needed.

Keywords

References

  1. Urist MR, Dowell TA, Hay PH, Strates BS. Inductive substrates for bone formation. Clin Orthop Relat Res. 1968;59:59-96
  2. Von Arx T, Broggini N, Jensen SS et al. Membrane Durability and Tissue Response of Different Bioresorbable Barrier Membranes: A Histologic Study in the Rabbit Calvarium. INT J ORAL MAXILLOFAC IMPLANTS 2005;20:843-853
  3. Hurzeler MB, Quinones CR, Hutmacher D, Schupbach P. Guided bone regeneration around dental implants in the atrophic alveolar ridge using a bioresorbable barrier. An experimental study in the monkey. Clin Oral Implants Res 8 1997;8:323-331 https://doi.org/10.1034/j.1600-0501.1997.080411.x
  4. Gher ME, Quintero G, Assad D, Monaco E, Richardson AC. Bone grafting and guided bone regeneration for immediate dental implants in humans. J Periodontol 1994;65:881-891 https://doi.org/10.1902/jop.1994.65.9.881
  5. Nowzari H, Slots J. Microbiologic and clinical study of polytetrafluoro-ethylene membranes for guided bone regeneration around implants. Int J Oral Maxillofac Implants 1995;10:67-73
  6. Price RL, Waidb MC, Haberstroha KM, Webstera TJ. Selective bone cell adhesion on formulations containing carbon nanofibers. Biomaterials 2003;24:1877-1787 https://doi.org/10.1016/S0142-9612(02)00609-9
  7. Sakabe H, Ito H, Miyamoto T, Noishiki Y, Ha WS. In vivo blood compatibility of regenerated silk fibroin. Sen-i Gakkaishi 1989;45:487-490 https://doi.org/10.2115/fiber.45.11_487
  8. Athreya SA, Martin DC. Impedance spectroscopy of protein polymer modified silicon micromachined probes. Sensors Actuators 1999;72:203-216 https://doi.org/10.1016/S0924-4247(98)00223-4
  9. Khil MS, Cha DI, Kim IS, Bhattarai N. Electrospun nanofibrous polyurethane membrane as wound dressing. J Biomed Mater Res 2003;67B:675-679 https://doi.org/10.1002/jbm.b.10058
  10. Bhattarai SR, Bhattarai N, Yi HK et al. Novel biodegradable electrospun membrane: scaffold for tissue engineering. Biomaterials 2004;25:2595-2602 https://doi.org/10.1016/j.biomaterials.2003.09.043
  11. Woodward SC, Brewer PS, Moatamed F. The intracellular degradation of poly(epsilon-caprolactone). J Biomed Mater Res 1985;19:437-444 https://doi.org/10.1002/jbm.820190408
  12. Hutmacher DW, Garcia AJ. Scaffold-based bone engineering by using genetically modified cells. Gene 2005;347:1-10 https://doi.org/10.1016/j.gene.2004.12.040
  13. Yohko G, Masuhiro T, Norihiko M. J Biomed Mater. Effect of the chemical modification of the arginyl residue in Bombyx mori silk fibroin on the attachment and growth of fibroblasts cells. J Biomed Mater Res 1998;39:351-357 https://doi.org/10.1002/(SICI)1097-4636(19980305)39:3<351::AID-JBM2>3.0.CO;2-I
  14. Yohko G, Masuhiro T, Norihiko M, Yohji I. Synthesis of poly(ethylene glycol)-silk fibroin conjugates and surface interaction between L-929 cells and the conjugates. Biomaterials 1997;18:267-271 https://doi.org/10.1016/S0142-9612(96)00137-8
  15. Minoura N, Aiba S, Gotoh Y, Tsukada M, Imai Y. Attachment and growth of cultured fibroblast cells on silk protein matrices. J Biomed Mater Res 1995;29:1215-1221 https://doi.org/10.1002/jbm.820291008
  16. Cai K, Yao K, Cui Y et al. Influence of different surface modification treatments on poly(d,l-lactic acid) with silk fibroin and their effects on the culture of osteoblast in vitro. Biomaterials. 2002;23:1603-1611 https://doi.org/10.1016/S0142-9612(01)00287-3
  17. Cai K, Yao K, Lin S et al. Poly(d,l-lactic acid) surfaces modified by silk fibroin: effects on the culture of osteoblast in vitro. Biomaterials 2002;23:1153-160 https://doi.org/10.1016/S0142-9612(01)00230-7
  18. Chen CS, Mrksich M, Huang S, Whitesides GM, Ingber DE. Science. Geometric control of cell life and death. Science 1997;276:1425-428 https://doi.org/10.1126/science.276.5317.1425
  19. van Kooten TG, Whitesides JF, von Recum AF. J Biomed Mater Res. Influence of silicone (PDMS) surface texture on human skin fibroblast proliferation as determined by cell cycle analysis. J Biomed Mater Res 1998;43:1-14 https://doi.org/10.1002/(SICI)1097-4636(199821)43:1<1::AID-JBM1>3.0.CO;2-T
  20. Min BM, Jeong L, Nam YS et al. Formation of silk fibroin matrices with different texture and its cellular response to normal human keratinocytes. Int J Biol Macromol 2004;34:223-230
  21. Kim KH, Jeong L, Park HN et al. Biological efficacy of silk fibroin nanofiber membranes for guided bone regeneration. J Biotechnology 2005;120:327-339 https://doi.org/10.1016/j.jbiotec.2005.06.033
  22. Becker W, Becker BE, Berg L et al. New attachment after treatment with root isolation procedures; report for treated class III and class II furcations and vertical osseous defects. Int J Periodontics Restorative Dent 1988;8:8-23
  23. Hutmacher DW, Kirsch A, Ackermann KL, Hurzeler MB. A tissue engineered cell-occlusive device for hard tissue regeneration-A preliminary report. Int J Periodontics Restorative Dent 2001;21:49-59
  24. Becker W, Becker BE, Berg L et al. New attachment after treatment with root isolation procedures; report for treated class III and class II furcations and vertical osseous defects. Int J Periodontics Restorative Dent 1988;8:8-23