Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Investigation of postnatal stem cells from canine dental tissue and bone marrow

성견 치계줄기세포 및 골수줄기세포 특성에 관한 연구

  • Jhin, Min-Ju (Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University, Department of Dentistry, Hanil General Hospital) ;
  • Kim, Young-Sung (Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University, Department of Periodontics, Asan Medical Center) ;
  • Kim, Su-Hwan (Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University, Department of Periodontics, Asan Medical Center) ;
  • Kim, Kyoung-Hwa (Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Lee, Chul-Woo (Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Koo, Ki-Tae (Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Kim, Tae-Il (Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Seol, Yang-Jo (Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Ku, Young (Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Rhyu, In-Chul (Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Chung, Chong-Pyoung (Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Lee, Yong-Moo (Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University)
  • 진민주 (서울대학교 치의학대학원 치주과학교실 및 치학연구소, 한일병원 치과) ;
  • 김영성 (서울대학교 치의학대학원 치주과학교실 및 치학연구소, 서울아산병원 치주과) ;
  • 김수환 (서울대학교 치의학대학원 치주과학교실 및 치학연구소, 서울아산병원 치주과) ;
  • 김경화 (서울대학교 치의학대학원 치주과학교실 및 치학연구소) ;
  • 이철우 (서울대학교 치의학대학원 치주과학교실 및 치학연구소) ;
  • 구기태 (서울대학교 치의학대학원 치주과학교실 및 치학연구소) ;
  • 김태일 (서울대학교 치의학대학원 치주과학교실 및 치학연구소) ;
  • 설양조 (서울대학교 치의학대학원 치주과학교실 및 치학연구소) ;
  • 구영 (서울대학교 치의학대학원 치주과학교실 및 치학연구소) ;
  • 류인철 (서울대학교 치의학대학원 치주과학교실 및 치학연구소) ;
  • 정종평 (서울대학교 치의학대학원 치주과학교실 및 치학연구소) ;
  • 이용무 (서울대학교 치의학대학원 치주과학교실 및 치학연구소)
  • Published : 2009.06.30
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Abstract

Purpose: The aim of this study was to evaluate the stemness of cells from canine dental tissues and bone marrow. Methods: Canine periodontal ligament stem cells (PDLSC), alveolar bone stem cells (ABSC) and bone marrow stem cells(BMSC) were isolated and cultured. Cell differentiations (osteogenic, adipogenic and chondrogenic) and surface antigens (CD146, STRO-1, CD44, CD90, CD45, CD34) were evaluated in vitro. The cells were transplanted into the subcutaneous space of nude mice to assess capacity for ectopic bone formation at 8 weeks after implantation. Results: PDLSC, ABSC and BMSC differentiated into osteoblasts, adipocytes and chondrocytes under defined condition. The cells expressed the mesenchymal stem cell markers differently. When transplanted into athymic nude mice, these three kinds of cells with hydroxyapatite /${\beta}$- tricalcium phosphate (HA/TCP) carrier showed ectopic bone formation. Conclusions: This study demonstrated that canine dental stem cells have stemness like bone marrow stem cells. Transplantation of these cells might be used as a therapeutic approach for dental stem cell-mediated periodontal tissue regeneration.

Keywords

References

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