Macromolecular Research

  • 제17권6호
  • /
  • Pages.424-429
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  • 2009
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  • 1598-5032(pISSN)
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  • 2092-7673(eISSN)
한국고분자학회 (The Polymer Society of Korea)
권호 표지

Biological Synthesis of Alkyne-terminated Telechelic Recombinant Protein

  • Ayyadurai, Niraikulam (School of Biotechnology, Yeungnam University) ;
  • Kim, So-Yeon (Institute for Environmental Technology and Industry, Pusan National University) ;
  • Lee, Sun-Gu (Institute for Environmental Technology and Industry, Pusan National University) ;
  • Nagasundarapandian, Soundrarajan (Department of Polymer Science and Chemical Engineering, Pusan National University) ;
  • Hasneen, Aleya (Department of Polymer Science and Chemical Engineering, Pusan National University) ;
  • Paik, Hyun-Jong (Department of Polymer Science and Chemical Engineering, Pusan National University) ;
  • An, Seong-Soo (Gachon BioNano Research Institute, Kyungwon University) ;
  • Oh, Eu-Gene (Graduate School of Global Entrepreneurship, Hoseo University)
  • 발행 : 2009.06.25
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초록

In this study, we demonstrate that the biological unnatural amino acid incorporation method can be utilized in vivo to synthesize an alkyne-terminated telechelic protein, Synthesis of terminally-functionalized polymers such as telechelic polymers is recognized to be important, since they can be employed usefully in many areas of biology and material science, such as drug delivery, colloidal dispersion, surface modification, and formation of polymer network. The introduction of alkyne groups into polymeric material is particularly interesting since the alkyne group can be a linker to combine other materials using click chemistry. To synthesize the telechelic recombinant protein, we attempted to incorporate the L-homopropargylglycine into the recombinant GroES fragment by expressing the recombinant gene encoding Met at the codons for both N- and C-terminals of the protein in the Met auxotrophic E. coli via Hpg supplementation. The Hpg incorporation rate was investigated and the incorporation was confirmed by MALDI-TOF analysis of the telcchelic recombinant protein.

키워드

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