Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Potential Application of the Recombinant Escherichia coli-Synthesized Heme as a Bioavailable Iron Source

  • Kwon, Oh-Hee (Department of Biotechnology, The Catholic University of Korea) ;
  • Kim, Su-Sie (Department of Biotechnology, The Catholic University of Korea) ;
  • Hahm, Dae-Hyun (Institute of Oriental Medicine and Acupuncture & Meridian Science Research Center, Kyung Hee University) ;
  • Lee, Sang-Yup (Department of Chemical and Biomolecular Engineering, KAIST) ;
  • Kim, Pil (Department of Biotechnology, The Catholic University of Korea)
  • Published : 2009.06.30
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Abstract

To investigate the potential use of microbial heme as an iron source, recombinant Escherichia coli coexpressing ALA synthase (HemA) as well as the NADP-dependent malic enzyme (MaeB) and dicarboxylic acid transporter (DctA) were cultured. The typical red pigment extracted from the recombinant E. coli after 38 h showed highest absorbance at 407 nm, and the amount of iron in 38.4 mg of microbial heme extract derived from 6-1 fermentation broth was 4.1 mg. To determine the commercial potential of the recombinant E.coli-synthesized iron-associated heme as an iron source, mice were fed the iron-free provender with the microbial heme extract. The average body weight reduction of mice fed non-iron provender was 2.3%, whereas no detectable weight loss was evident in mice fed microbial heme addition after 15 days. The heme content of the blood from microbial heme fed mice was 4.2 mg/ml whereas that of controls was 2.4 mg/ml, which implies that the microbial heme could be available for use as an animal iron source.

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