Journal of Forest and Environmental Science

Institute of Forest Science, kangwon National University (강원대학교 산림과학연구소)
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Agrobacterium tumefaciens-Mediated Genetic Transformation: Mechanism and Factors

  • Kumar, Nitish (Department of Agricultural Biotechnology, Anand Agricultural University) ;
  • Vijayanand, K.G. (Discipline of wasteland research, Central Salt & Marine Chemicals Research Institute (Council of Scientific and Industrial Research)) ;
  • Reddy, Myppala P. (Plant Stress Genomics and Technology Center, King Abdullah University of Science and Technology) ;
  • Singh, Amritpal S. (Department of Agricultural Biotechnology, Anand Agricultural University) ;
  • Naraynan, Subhash (Department of Agricultural Biotechnology, Anand Agricultural University)
  • Received : 2009.11.04
  • Accepted : 2009.12.26
  • Published : 2009.12.31
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Abstract

Agrobacterium-mediated genetic transformation has been widely used for the production of genetically modified transgenic plants to obtain specific desired traits. Most of the molecular mechanisms that underlie the transformation steps have been well elucidated over the years. However, a few steps, such as nuclear targeting, T-DNA integration, and Agrobacterium-plant proteins involved remain largely obscure and are still under extensive studies. This review describes the major steps involved in the molecular mechanism of Agrobacterium-mediated transformation and provides insight in the recent developments in studies on the Agrobacterium-mediated genetic transformation system. Some factors affecting the transformation efficiency are also briefly discussed.

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