Royal Jelly Protein and Lipid Composition in Apis cerana indica F.

  • 발행 : 2009.06.30

초록

The histological and transmission electron microscopic studies revealed the synthesis activity predominantly in the hypopharyngeal glands of the nurse bees. The biochemical analysis of both, the hypopharyngeal gland extract and royal jelly elucidated unequivocally the proteins and lipids as the major constituents. Further the SDS-PAGE of hypopharyngeal gland extract showed about 17 protein bands, perhaps 14.10, 20.00, 29.00 and 43.00 kDa predominantly while that of royal jelly revealed only two protein bands of 29.00 and 43.00 kDa molecular weight suggesting them as the major royal jelly proteins (MRJP). The lipid profile of royal jelly consists of triglycerides, cholesterol, HDL, LDL and VLDL.

키워드

참고문헌

  1. Albert S, Bhattacharya D, Klaudiny J, Schmitzova J, $\check{S}$imuth J (1999) The family of major royal jelly proteins and its evolution. J Mol Evol 49, 290-297 https://doi.org/10.1007/PL00006551
  2. Alliain CC, Poon LS, Chan CSG, Richmond W, Fu PC (1974) Enzymatic determination of total serum cholesterol. Clin Chem 20, 470-475
  3. Annoni G, Bottasso BM, Ciaci D, Donato MF, Tripoli A (1982) Evaluation of a new enzymatic method for triglyceride estimation. J Res Lab Med 9, 115
  4. Brouwers EVM (1982) Measurement of hypopharyngeal gland activity in the honeybee. J Apic Res 21, 193-198 https://doi.org/10.1080/00218839.1982.11100541
  5. Cruz-Landim C da, Hadek R (1969) Ultrastructure of Apis mellifera hypopharyngeal gland. Proc 6$^{th}$ Int Congr IUSSI, Bern, 121-130
  6. Deseyn J, Billien J (2005) Age-dependent morphology and ultrastructure of the hypopharyngeal gland of Apis mellifera workers (Hymenoptera, Apidae). Apidologie 36, 49-57 https://doi.org/10.1051/apido:2004068
  7. Echigo T, Takenaka T, Yatsunami K (1986) Comparative studies on chemical composition of honey, royal jelly and pollen loads. Bull Faculty of Agric, The Tamagawa University, 26, 1-12
  8. Friedwald WT, Levy RT, Fredickson DS (1972) Estimation of the concentration of low density lipoprotein cholesterol in plasma, without use of preparative ultracentrifuge. Clin Chem 18, 499-502
  9. Hanes J, Šimuth J (1992) Identification and partial characterization of major royal jelly protein of honeybee (Apis mellifera L.). J Apic Res 31, 22-26 https://doi.org/10.1080/00218839.1992.11101256
  10. Howe SR, Dimick PS, Benton AW (1985) Composition of freshly harvested and commercial royal jelly. J Apic Res 24, 52-61 https://doi.org/10.1080/00218839.1985.11100649
  11. Kamakura M, Suehobu N, Fukushima M (2001) Fifty seven KD protein in royal jelly enhances proliferation of primary culture of hepatocytes and increases albumin production in the absence of serum. J Biochem Biophys Res Commun 13, 282, 865-874
  12. Knecht D, Kaatz HH (1990) Patterns of larval food production by hypopharyngeal glands in adult worker honeybees. Apidologie 21, 457-468 https://doi.org/10.1051/apido:19900507
  13. Kubo T, Sasaki M, Nakamura J, Sasagawa H, Ohashi K, Takuchi H (1996) Change in the expression of hypopharyngeal gland proteins of the worker honeybees (Apis mellifera L.) with the age and/or role. J Biochem 119, 291-295 https://doi.org/10.1093/oxfordjournals.jbchem.a021237
  14. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685 https://doi.org/10.1038/227680a0
  15. Lerker G, Capella P, Conte LS, Ruini F, Giordani G (1982) Components of royal jelly, II. The lipid fraction, hydrocarbons and sterols. J Apic Res 21, 178-184 https://doi.org/10.1080/00218839.1982.11100538
  16. Michener CD (1974) The social behaviour of the Bees. Harvard University Press, Cambridge, Massachusetts
  17. Sato O, Kunikata T, Kohno K, Iwaki K, Ikeda M, Kurimoto M (2004) Characterization of royal jelly proteins in both Africanized and European honeybees (Apis mellifera) by two dimensional gel electrophoresis. J Agric Food Chem 52, 15-20 https://doi.org/10.1021/jf030340e
  18. Schimitzova J, Klaudiny J, Albert S, Schroder W, Schreckngost W, Hanes J, Judova J, $\check{S}$imuth J (1998) A new family of major royal jelly proteins of honeybee Apis mellifera. L. Cell Mol Life Sci 54, 1020-1030 https://doi.org/10.1007/s000180050229
  19. $\check{S}$imuth J (2001) Some properties of the main protein of honeybee(Apis mellifera) royal jelly. Apidologie 32, 69-80 https://doi.org/10.1051/apido:2001112
  20. Takenaka T, (1982) Chemical composition of royal jelly. Honeybee Sci 3, 69-74
  21. Tembhare DB (2008) Techniques in Life Sciences. Himalaya Publ. House, Mumbai India
  22. Wilson EO (1971) The insect societies. Belknap Press of Harvard University, Cambridge