International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
권호 표지

Study on Productivity and Genotype Structure by Several Enzyme Loci of Silkworm(Bombyx mori L.) Parthenoclones Obtained by Thermal and Combined(Low-High Temperature) Method

  • Published : 2009.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to establish whether there are differences in the productivity of the same silkworm(Bombyx mori L.) parthenoclones, obtained by two different methods-thermal and combined, as well as to study their genotype structure by several enzyme loci. It was established that all individuals of parthenoclones Joana, Joana(${\downarrow}{\uparrow}$), Pohi and Pohi(${\downarrow}{\uparrow}$), are homozygous by the studied esterase and phosphoglucomutase loci, which substantiated the clones' genetic stability. By comparative analysis of some biological and technological properties, it was found that parthenoclone Pohi(${\downarrow}{\uparrow}$) obtained by low-high temperature activation is characterized by higher values of these properties as compared to parthenoclone Pohi obtained by thermal parthenogenesis. Comparing the two methods of inducing ameiotic parthenogenetic development, we would recommend that parthenoclone Joana is sustained by thermal parthenogenesis, and parthenoclone Pohi-by the combined method (low-high temperature).

Keywords

References

  1. Klimenko V (1980) Induced parthenogenesis mechanism for silkworm. Message I. Interdependency between the thermal activation effect and the speed of postactivation cooling. Genetics XVI, 1002-1010. (in Russian)
  2. Klimenko V (1980) Induced parthenogenesis mechanism for silkworm. Message II. Reactivation of unfertilized eggs deactivated by gradual cooling after thermal treatment. Genetics XVI, 1010-1019. (in Russian)
  3. Murakami A (1985) Genetic studies on parthenogenesis in B. mori: thermal activation and its significance. Annu Rept Nat Inst Genet Jap 36, 48-49
  4. Singh R, Raghdvendra R, Karappa B, Snyal P, Premalatha, V. (2002) Studies on parthenogenetic development, hybrid vigour and cocoon shape variability in bivoltine F1 hybrids of the silkworm, B. mori L. Int J Indust Entomol 5, 59-65
  5. Shaw C, Prasad R (1970) Starch gel electrophoresis of enzymes – A compilation of Recipeds. Biochem Genet 4, 297-320 https://doi.org/10.1007/BF00485780
  6. Spencer N, Hopkinson D, Harris H (1964) Phosphoglucomutase polymorphism in man. Nature 204, 742-745 https://doi.org/10.1038/204742a0
  7. Staykova T, Grekov D, Panayotov M (2004) Electrophoretic analysis of nonspecific esterases in silkworm (Bombyx mori L.) female genital organs and eggs. Int J Indust Entomol 9, 59-63
  8. Stoykova T, Popov P, Dimitrov B (2003) Electrophoretic analysis of non-specific haemolymph esterases during silkworm (Bombyx mori L.) ontogenesis. Sericologia 43, 153-162
  9. Staykova T, Grekov D (2006) Stage specificity and polymorphism of haemolymph esterases in races and hybrids of silkworm (Bombyx mori L.) kept in Bulgaria. Proceedings of the International workshop on silk handcrafts cottage industris and silk enterprises development in Africa, Europe, Central Asia and the Near East & Second executive meeting of Black, Caspian seas and Central Asia Silk Assosiation (BACSA), Bursa, Turkey, 6-10 March, 667-674
  10. Staykova T. (2008) Genetically-determined polymorphism of nonspecific esterases and phosphoglucomutase in eight introduced breeds of the silkworm, Bombyx mori, raised in Bulgaria. J Insect Sci 8, 18
  11. Strunnikov V, Terskaya E, Maresin V, Demyanov E (1980) On the nature of activating silkworm eggs to parthenogenetic development. ANSSSR Reports 253, 1247-1251. (in Russian)
  12. Strunnikova L. Demyanov E (1980) Activation of eggs laid by female silkworm to parthenogentic development. Silk 3, 9-10. (in Russian)
  13. Sugai E, Aoyagi H, Otsuka K (1983) Some fundamental studies on parthenogenesis of the silkworm ovarian eggs B. mori L. J Seric Sci Jap 52, 51-56
  14. Takei R, Nakagaki R, Kodoira, Nagashima E (1990) Factor analysis on the parthenogenetic development of ovarian eggs in the silkworm, B. mori L. (Lepidoptera, Bombycidae). Appl Entomol Zool 25, 43-48 https://doi.org/10.1303/aez.25.43
  15. Vasileva Y (2006) Study on methods for obtaining and breeding parthenogenetic silkworm individuals (Bombyx mori L.). Ph. D. Thesis, Bulgarian Agricultural Academy. (in Bulgarian)
  16. Vereyskaya V (1979) Cytology of silkworm egg maturing (B. mori L.) at induced parthenogenesis. Ontogenesis 10, 244-251. (in Russian)
  17. Yonqiang W, Mengkui X, Jianguo X (2001) Studies on parthenogenesis of commercial silkworm races. Acta Seric Sin 27, 20-23