Nutrition Research and Practice

The Korean Nutrition Society (한국영양학회)
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The inhibition of inflammatory molecule expression on 3T3-L1 adipocytes by berberine is not mediated by leptin signaling

  • Choi, Bong-Hyuk (School of Life Science, Handong Global University) ;
  • Kim, Yu-Hee (School of Life Science, Handong Global University) ;
  • Ahn, In-Sook (School of Life Science, Handong Global University) ;
  • Ha, Jung-Heun (School of Life Science, Handong Global University) ;
  • Byun, Jae-Min (School of Life Science, Handong Global University) ;
  • Do, Myoung-Sool (School of Life Science, Handong Global University)
  • Published : 2009.06.30
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Abstract

In our previous study, we have shown that berberine has both anti-adipogenic and anti-inflammatory effects on 3T3-L1 adipocytes, and the anti-adipogenic effect is due to the down-regulation of adipogenic enzymes and transcription factors. Here we focused more on anti-inflammatory effect of berberine using real time RT-PCR and found it changes expressions of adipokines. We hypothesized that anti-adipogenicity of berberine mediates anti-inflammtory effect and explored leptin as a candidate mediator of this signaling. We studied this hypothesis by western blot analysis, but our results showed that berberine has no effect on the phosphorylations of STAT-3 and ERK which have important roles on leptin signaling. These results led us to conclude that the anti-inflammatory effect of berberine is not mediated by the inhibition of leptin signal transduction. Moreover, we have found that berberine down-regulates NF-${\kappa}B$ signaling, one of the inflammation-related signaling pathway, through western blot analysis. Taken together, the anti-inflammatory effect of berberine is not mediated by leptin, and berberine induces anti-inflammatory effect independent of leptin signaling.

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