환도(環跳)($GB_{30}$) 초오약침(草烏藥鍼)이 L5 척수신경 결찰에 의한 흰쥐의 동통모델에 미치는 영향

Effects of Radix Aconiti Herbal Acupuncture Injected at Hwando($GB_{30}$) on Neuropathic Pain in Rats

  • 방성필 (동신대학교 한의과대학 침구학교실) ;
  • 류미선 (동신대학교 한의과대학 침구학교실) ;
  • 김재홍 (동신대학교 한의과대학 침구학교실) ;
  • 위통순 (동신대학교 한의과대학 침구학교실) ;
  • 윤대환 (동신대학교 한의과대학 경혈학교실) ;
  • 윤여충 (동신대학교 한의과대학 침구학교실) ;
  • 여경찬 (국립의료원 침구과) ;
  • 정호석 (자생한방병원 침구과)
  • Bang, Sung-Pil (Department of Acupuncture and Moxibustion, College of Oriental Medicine, Dongshin University) ;
  • Ryu, Mi-Seon (Department of Acupuncture and Moxibustion, College of Oriental Medicine, Dongshin University) ;
  • Kim, Jae-Hong (Department of Acupuncture and Moxibustion, College of Oriental Medicine, Dongshin University) ;
  • Wei, Tung-Sheun (Department of Acupuncture and Moxibustion, College of Oriental Medicine, Dongshin University) ;
  • Yun, Dae-Hwan (Department of Acupoint, College of Oriental Medicine, Dongshin University) ;
  • Yun, Yeo-Choong (Department of Acupuncture and Moxibustion, College of Oriental Medicine, Dongshin University) ;
  • Yeo, Kyeong-Chan (Department of Acupuncture and Moxibustion, National Medical Center) ;
  • Jung, Ho-Seok (Department of Acupuncture and Moxibustion, Jaseng Hospital of Oriental Medicine)
  • 투고 : 2009.11.04
  • 심사 : 2009.11.17
  • 발행 : 2009.12.20

초록

Objectives : The purpose of this study is to examine if Radix Aconiti herbal acupuncture may be effective to the neuropathic pain(mechanical allodynia, cold allodynia) in a rat model of neuropathic pain. Methods : To produce the model of neuropathic pain, under isoflurane 2.5% anesthesia, L5 spinal nerve was ligated by 6-0 silk thread. After neurophathic surgery, the author examined if the animals exhibited the behavioral sign of allodynia. The allodynia was assessed by stimulating the medial malleolus with von frey filament and acetone. Three days after the neuropathic surgery, Radix Aconiti herbal acupuncture was injected at Hwando($GB_{30}$) one time per two days for 2 weeks. After that, the author examined the withdrawal response of neuropathic rats' legs by von frey filament and acetone stimulation. And also the author examined c-Fos in the midbrain central gray of neuropathic rats and the change of WBC, RBC, HGB, HCT count in the blood of neuropathic rats. Results : The Radix Aconiti herbal acupuncture injected Hwando($GB_{30}$) decreased the withdrawal response of mechanical allodynia that assessed with von frey filament in RA1-$GB_{30}$, RA2-$GB_{30}$ group as compared with control group. The Radix Aconiti herbal acupuncture injected Hwando($GB_{30}$) decreased the withdrawal response of chemical allodynia(cold allodynia) that assessed with acetone in RA1-$GB_{30}$, RA2-$GB_{30}$ group as compared with control group. The Radix Aconiti herbal acupuncture injected Hwando($GB_{30}$) showed the significant between control group and RA2-$GB_{30}$ group in the c-Fos expression. The Radix Aconiti herbal acupuncture injected Hwando($GB_{30}$) did not show the significant between control group and RA1-$GB_{30}$ group, RA2-$GB_{30}$ group and RA3-$GB_{30}$ group in the WBC, RBC, HGB, HCT count. Conclusions : We have noticed that Radix Aconiti herbal acupuncture at Hwando($GB_{30}$) decreased mechanical allodynia and cold allodynia in the model of neuropathic pain compared with the control group. C-fos expression in the central gray of that group was also decreased compared with the control group. This study can be used as a basic resource on a study and a treatment of pain.

키워드

참고문헌

  1. Devor M. Nerve pathophysiology and mechanisms of pain in causalgia. J Auton Nerv syst. 1983 ; 7 : 371-84. https://doi.org/10.1016/0165-1838(83)90090-5
  2. Bonica JJ. Causalgia and other reflex sympathetic dystrophies. The management of pain. Edited by Bonica JJ. Pennsylvania : Lea and Febiger Publishers. 1990 : 220-43.
  3. 이배환. 신경병리성 동통의 조절에 있어서 아드레날린계와 아편계의 상호작용. 한국심리학회지 : 생물 및 생리. 2001 ; 13(1) : 31-43.
  4. Baron R. Peripheral neuropathic pain from mechanisms to symptoms. Clinical Journal of Pain. 2000 ; 16(2) : S12-S20. https://doi.org/10.1097/00002508-200006001-00004
  5. 대한약침학회 학술위원회. 약침학. 서울. 엘스비어코리아. 2008 : 3, 6-8.
  6. 신민교. 臨床本草學. 서울. 永林社. 1997 : 302-4.
  7. 全國韓醫科大學 鍼灸經穴學敎室. 鍼灸學(上). 파주 : 集文堂. 1998 : 243, 643-5.
  8. 김성철. 환도혈 오공약침 자극이 백서의 신경병리성 통증 억제에 미치는 영향. 대한침구학회지. 2004 ; 21(3) : 145-67.
  9. 이동현. 환도혈 봉독 약침 자극이 백서의 신경병리성 동통 억제에 미치는 영향. 대한침구학회지. 2005 ; 22(5) : 67-77.
  10. 정정희. 건측 취혈 다종 침자법이 백서의 신경병리성 동통에 미치는 영향. 대한침구학회지. 2007 ; 24(5) : 137-50.
  11. 정정희. 후계, 위중, 후계배위중 침자가 백서의 신경병리성 동통억제 및 c-Fos 단백 발현에 미치는 영향. 대한침구학회지. 2004 ; 21(1) : 240-51.
  12. 오창록. 위중, 후계, 위중배후계 전침 및 침자가 백서의 신경병리성 동통 억제에 미치는 영향. 대한침구학회지. 2005 ; 22(1) : 77-90.
  13. 윤덕미. 신경병적 통증. 대한통증학회지. 1995 ; 8(2) : 219-24.
  14. Bennett GJ, Xie YK. A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man. Pain. 1988 ; 33(1) : 87-107. https://doi.org/10.1016/0304-3959(88)90209-6
  15. Seltzer Z, Dubner R, Shir Y. A novel behavioral model of neuropathic pain disorders produced in rats by partial sciatic nerve injury. Pain. 1990 ; 43(2) : 205-18. https://doi.org/10.1016/0304-3959(90)91074-S
  16. Kim SH, Chung JM. An experimental modal for peripheral neuropathy produced by segmental spinal nerve ligation in the rat. Pain. 1992 ; 50(3) : 355-63. https://doi.org/10.1016/0304-3959(92)90041-9
  17. DeLeo JA, Coombs DW, Willenbring S, Colburn RW, Fromm C, Wagner R, Twitchell BB. Characterization of a neuropathic pain model: Sciatic cryoneurolysis in the rat. Pain. 1994 ; 56(1) : 9-16. https://doi.org/10.1016/0304-3959(94)90145-7
  18. 河北醫學院 編著. 靈樞經校釋(下冊) 刺節眞邪篇. 北京 : 人民衛生出版社. 1982 : 352.
  19. 楊維傑 篇. 黃帝內經 素問 靈樞 解釋. 서울 : 성보사. 1980 : 23-50.
  20. 丁光迪 主編. 諸病源候論校注(上). 北京 : 人民衛生出版社. 1992 : 152.
  21. 木下晴都. 통증과 침구치료. 대구 : 동양종합통신교육출판부. 1982 : 231-2.
  22. 木下晴都. 좌골신경통과 침구치료. 대구 : 동양종합통신교육출판부. 1985 : 19-22, 33-54.
  23. 丁光迪 主編. 諸病源候論校注(下). 北京 : 人民衛生出版社. 1992 : 854.
  24. 安榮基. 경혈학총서. 서울 : 성보사. 2002 : 578-9.
  25. 張隱庵 外. 本草三家合註. 서울 : 성보출판사. 1981 : 164.
  26. 盧富來, 成樂箕. 생초오와 법제초오수침이 흰쥐의 Adjuvant 관절염에 미치는 영향. 대한침구학회지. 1994 ; 11(1) : 435-50.
  27. Sauviat MP. Effects of neurotoxins on the electrical activity and contraction of the heart muscle. C R Seance Soc Boil Fil. 1997 ; 191(3) : 451-71.
  28. Shir Y, Seltzer Z. Effects of sympathectomy in a model of causalgiform pain produced by partial sciatic nerve injury in rats. Pain. 1991 ; 45(3) : 309-20. https://doi.org/10.1016/0304-3959(91)90056-4
  29. Heavner JE, Calvillo O, Racz GB. Thermal grill illusion and complex regional pain syndrome type I (reflex sympathetic dystrophy). Reg Anesth. 1997 ; 22(3) : 257-9. https://doi.org/10.1016/S1098-7339(06)80011-8
  30. Wahren LK, Torebjork E, Nystrom B. Quantitative sensory testing before and after regional guanethidine block in patients with neuralgia in the hand. Pain. 1991 ; 46(1) : 23-30. https://doi.org/10.1016/0304-3959(91)90029-W
  31. Robertson GS, Fibiger HC. Neuroleptics increase c-Fos expression in the forebrain : contrasting effects of haloperidol and clozapine. Neuroscience. 1992 : 46(2) : 315-28. https://doi.org/10.1016/0306-4522(92)90054-6
  32. Schilling K, Curran T, Morgan JI. The excitement of immediate-early genes. Ann NY Acod Sci. 1991 ; 627 : 115-23. https://doi.org/10.1111/j.1749-6632.1991.tb25917.x
  33. Bullitt E. Expression of c-fos-like protein as a marker for neuronal activity following noxious stimulation in the rat. J Comp Neurol. 1990 ; 296(4) : 517-30. https://doi.org/10.1002/cne.902960402