Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
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The Effects of Needle Electrode Electrical Stimulation on Cellular Necrosis Blocking the Hippocampus after Induction of Ischemia

침전극저주파 자극이 해마의 세포사 차단에 미치는 영향

  • Lee, Jung-Sook (Department of Occupation Therapy, Dong-ju, College University) ;
  • Kim, Byung-Ki (Department of Molecular Biology, Dong-eui university) ;
  • Kim, Sung-Won (Department of Occupation Therapy, Han-yeong College University) ;
  • Kim, Ji-Sung (Department of Physical Therapy, Suwon Women's College) ;
  • Kim, Dong-Il (Department of Ob&Gy Dongguk Univ. Iisan Korean Medicine Hospital) ;
  • Song, Chi-Won (Division of General Services, National Institute of Toxicological Research)
  • 이정숙 (동주대학교 작업치료학과) ;
  • 김병기 (동의대학교 생물학과) ;
  • 김성원 (한영대학교 작업치료학과) ;
  • 김지성 (수원여자대학교 물리치료학과) ;
  • 김동일 (동국대학교 부인학과) ;
  • 송치원 (국립독성과학원 연구지원과)
  • Published : 2009.06.30
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Abstract

Objectives : This study was performed to investigate the effects of Needle Electrode Electrical Stimulation (NEES) on ischemia-induced cerebrovascular accidents. After obstruction and reperfusion of ${\ast}{\ast}$ arteries in white mice, the amounts of necrosis and inflammation related substances IL-6, Caspase-3, and PARP, C-fos were measured in neurons of the hippocampus. The following results were obtained. Methods : This study used 21 male specific pathogen free (SPF) SD (Sprague Dawley) rats, 8 weeks of age and approximately 300 g in weight, that were given at least 1 week to adapt to the lab environment Each exposed artery was completely occluded with non-absorbent suture thread and kept in that state for 5 minutes. The sutures were then removed to allow reperfusion of blood. Test group is control group for comparison with the common carotid artery occlusion models, a GI group that underwent common carotid artery occlusion, and a needle electrode electrical stimulation (NEES) group that underwent NEES after artery occlusion. The GI and NEES groups were given 12, 24, or 48 hours of reperfusion before NEES. NEES device (PG6, ITO, Japan, 9V, current, 2Hz) was used to stimulate the right and left acupoint ST36 of the SD rats for 30 minutes while they were sedated with 3% isoflurane. An immunohistochemistry test was done on the forebrains of the GI induced rats. All the data collected from this study was symbolized and analyzed using a statistics processing program (SPSS 12.0K/PC). The level of significance was set at ${\alpha}$=0.05 and a T-TEST analysis was used to find out the effects of treatment on each of the groups: the normal group, the CVA induced group, and the treatment after CVA induction group. Results : Both PARP and C-fos immuno-reactive cells, related to apoptosis, were greater in the GI groups than the NEES group. Caspase and IL-6 immuno-reactive cells, related to inflammation, were greater in the GI and NEES groups than the control group. Conclusions : This research was conducted to study the effects of NEES on CVA due to ischemia. Occlusion and reperfusion was performed on the common carotid arteries of white rats, after which amounts of substances related to neuron necrosis and inflammation - PARP, IL-6, Caspase-3, and C-fos - were measured in the Hippocampus

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