동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제23권3호
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  • Pages.673-677
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  • 2009
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  • 1738-7698(pISSN)
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  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

RAW 264.7 대식세포에서 Gelidium amansii의 항염증 효과

Anti-inflammatory Effects of Gelidium amansii in RAW 264.7 Macrophages

  • 최원식 (순천향대학교 생명공학과) ;
  • 김영선 (순천향대학교 생명공학과) ;
  • 이상현 (원광대학교 한의과대학 방제학교실) ;
  • 채규윤 (원광대학교 생명나노화학부) ;
  • 이영행 (원광대학교 생명나노화학부)
  • Choi, Won-Sik (Department of Biotechnology, Soonchunhyang University) ;
  • Kim, Young-Sun (Department of Biotechnology, Soonchunhyang University) ;
  • Lee, Sang-Hyun (Department of Oriental Medical Prescription, College of Oriental Medicine, Wonkwang University) ;
  • Chai, Kyu-Yun (Division of Nanobiochemistry, Wonkwang University) ;
  • Lee, Young-Haeng (Division of Nanobiochemistry, Wonkwang University)
  • 발행 : 2009.06.25
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초록

In order to verify the anti-inflammatory effects of Gelidium amansii, RAW264.7 macrophages were incubated with the extract of 70% ethanol solution (Ex), and activated with the endotoxin lipopolysaccharide (LPS). Ex inhibited the expression of the pro-inflammatory enzymes, including inducible nitric oxide (NO) synthase (iNOS) and cyclooxygenase-2 (COX-2), and the production of iNOS-mediated NO and COX-2-mediated prostglandin $E_2$ ($PGE_2$) production in a dose-dependent manner. Ex also reduced the release of the pro-inflammatory cytokines, including tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-1${\beta}$ (IL-1${\beta}$) and IL-6 in LPS-activated macrophages, The observed anti-inflammatory effects of Ex was associated with inactivation of the nuclear factor ${\kappa}B$ (NF-${\kappa}B$) that mediates the induction of iNOS, COX-2, TNF-${\alpha}$, IL-1${\beta}$, and IL-6. Further studies showed that Ex inactivated NF-${\kappa}B$ through inhibition of phosphorylation of the inhibitory ${\kappa}B$ ($l{\kappa}B$), Taken together, these results suggest that Gelidium amansii exerts anti-inflammatory effects by inhibiting the expression of pro-inflammatory enzymes and the secretion of pro-inflammatory cytokines via inactivation of NF-${\kappa}B$ and/or $l{\kappa}B$.

키워드

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