Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Soyangin-Hyeongbangpaedok-san on Anti-CD3 Stimulated Mouse T Cells In Vivo and In Vitro

소양인 형방패독산(荊防敗毒散)의 마우스 T 세포 활성에 대한 실험적 연구

  • Kim, Joong-Bae (Department of Pathology, College of Oriental Medicine & Institute of Oriental Medicine, Kyunghee University) ;
  • Kang, Hee (Department of East-West Medical Science, Graudate School of East-West Medical Science, Kyunghee University) ;
  • Ahn, Kwang-Seok (Department of Pathology, College of Oriental Medicine & Institute of Oriental Medicine, Kyunghee University) ;
  • Shim, Bum-Sang (Department of Pathology, College of Oriental Medicine & Institute of Oriental Medicine, Kyunghee University) ;
  • Kim, Sung-Hoon (Cancer Preventive Material Development Research Center, Kyunghee University) ;
  • Choi, Seung-Hoon (Department of Pathology, College of Oriental Medicine & Institute of Oriental Medicine, Kyunghee University) ;
  • Ahn, Kyoo-Seok (Department of Pathology, College of Oriental Medicine & Institute of Oriental Medicine)
  • 김중배 (경희대학교 한의과대학 병리학교실 & 경희대학교 한의학 연구소) ;
  • 강희 (경희대학교 동서의학대학원 동서의과학) ;
  • 안광석 (경희대학교 한의과대학 병리학교실 & 경희대학교 한의학 연구소) ;
  • 심범상 (경희대학교 한의과대학 병리학교실 & 경희대학교 한의학 연구소) ;
  • 김성훈 (암예방소재개발연구센터) ;
  • 최승훈 (경희대학교 한의과대학 병리학교실 & 경희대학교 한의학 연구소) ;
  • 안규석 (경희대학교 한의과대학 병리학교실 & 경희대학교 한의학 연구소)
  • Published : 2009.06.25
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Abstract

Soyangin-Hyeongbangpaedok-san(SHBPDS) is a herbal formula used for the common cold or upper respiratory illness. In order to investigate the effect of SHBPDS, mice were orally administered with SHBPDS alcohol extract for 7 days followed by intravenous anti-CD3 injection. In addition, splenocytes and CD4 T cells were cultured with SHBPDS in response to anti-CD3 in vitro and cytokines and transcription factors were evaluated. In vivo treatment with SHBPDS significantly augmented the expressions of the percentage of CD4 T cells and CD 69, an indicator of early T cell activation. Serum levels of IL-4 were significantly increased but those of IFN-${\gamma}$ and IL-2 did not reach statistical significance. The expressions of IFN-${\gamma}$ and T-bet mRNA were significantly downregulated in SHBPDS treated mice while those of IL-4 and C-Maf were significantly upregulated. In vitro stimulation of splenocytes and CD4 T cells by SHBPDS resulted in a reduction in IFN-${\gamma}$ secretion and STAT4 activity. The IL-4 releases from both cells were slightly reduced, but STAT6 activity was rather increased. In conclusion, SHBPDS exerted an inhibition in the expression of IFN-${\gamma}$, T-bet and STAT4 while IL-4, C-Maf and STAT6 were increased. Further studies are required to examine its pharmacological effects using more appropriate animal experiments.

Keywords

References

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