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Swiprosin-1 Regulates Cytokine Expression of Human Mast Cell Line HMC-1 through Actin Remodeling

  • Ramesh, T.P. (Department of Life Science, Gwangju Institute of Science and Technology) ;
  • Kim, Young-Dae (Department of Life Science, Gwangju Institute of Science and Technology) ;
  • Kwon, Min-Sung (Department of Life Science, Gwangju Institute of Science and Technology) ;
  • Jun, Chang-Duk (Department of Life Science, Gwangju Institute of Science and Technology) ;
  • Kim, Sang-Wook (Department of Internal Medicine, Chonbuk National University College of Medicine)
  • Received : 2009.12.09
  • Accepted : 2009.12.21
  • Published : 2009.12.31
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Abstract

Background: Swiprosin-1 was identified in human CD8+ lymphocytes, mature B cells and non-lymphonoid tissue. We have recently reported that swiprosin-1 is expressed in mast cells and up-regulated in both in vitro and in vivo. Methods: The expression of cytokines and swiprosin-1 were determined by by real time PCR and conventional PCR. Pharmacological inhibitors were treated to investigate potential mechanism of swiprosin-1 in mast cell activation. Actin content was evaluated by confocal microscopy and flow cytometry. Results: The swiprosin-1 augmented PMA/A23187-induced expression of cytokines and release of histamine. However, knock-down of swiprosin-1 showed only a modest effect on PMA/A23187-induced cytokine expression, suggesting that swiprosin-1 has gain-of-function characteristics. Swiprosin-1 was found in microvilli-like membrane protrusions and highly co-localized with F-actin. Importantly, either disruption of actin by cytochalasin B or inhibition of PI3 kinase, an enzyme involved in actin remodeling, by wortmannin blocked cytokine expression only in swiprosin-1-overexpressing cells. Conclusion: These results suggest that swiprosin-1 modulates mast cell activation potentially through actin regulation.

Keywords

References

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