Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Detection of Cytolethal Distending Toxin and Other Virulence Characteristics of Enteropathogenic Escherichia coli Isolates from Diarrheal Patients in Republic of Korea

  • Kim, Jong-Hyun (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health) ;
  • Kim, Jong-Chul (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health) ;
  • Choo, Yun-Ae (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health) ;
  • Jang, Hyun-Chul (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health) ;
  • Choi, Yeon-Hwa (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health) ;
  • Chung, Jae-Keun (Gwang-Ju Institute of Health and Environment) ;
  • Cho, Seung-Hak (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health) ;
  • Park, Mi-Seon (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health) ;
  • Lee, Bok-Kwon (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health)
  • Published : 2009.05.31
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Abstract

Cytolethal distending toxins (CDTs) represent an emerging family of newly described bacterial products that are produced by a number of pathogens. The genes encoding these toxins have been identified as a cluster of three adjacent genes, cdtA, cdtB, and cdtC, plus 5 cdt genetic variants, designated as cdt-I, cdt-II, cdt-III, cdt-IV, and cdt-V, have been identified to date. In this study, a general multiplex PCR system designed to detect Escherichia coli cdts was applied to investigate the presence of cdt genes among isolates. As a result, among 366 E. coli strains, 2.7% were found to carry the cdtB gene. In addition, the use of type-specific primers revealed the presence of cdt-I, cdtIV, and cdt-V types of the cdt gene, yet no cdt-II or cdt-III strains. The presence of other virulence genes (stxl, stx2, eae, bfp, espA, espB, and espD) was also investigated using a PCR assay. Among the 10 cdtB gene-positive strains, 8 were identified as COT-producing typical enteropathogenic E. coli (EPEC) strains ($eae^+$, $bfp^+$), whereas 2 were identified as CDT-producing atypical EPEC strains ($eae^+$, $bfp^-$). When comparing the cytotoxic activity of the CDT-producing typical and atypical EPEC strains, the CDT-producing atypical EPEC strains appeared to be less toxic than the CDT-producing typical EPEC strains.

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References

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