Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Commercial Application of Porcine MC1R Gene Polymorphisms to Korean Pork Industry

돼지 MC1R 유전자변이의 양돈산업 적용

  • Ha, You-Kyoung (Department of Animal Science, Chungbuk National University) ;
  • Choi, Jung-Suk (Department of Animal Science, Chungbuk National University) ;
  • Kim, Sang-Wook (Department of Animal Science, Chungbuk National University) ;
  • Choi, Yang-Il (Department of Animal Science, Chungbuk National University) ;
  • Lee, Seug-Soo (National Livestock Research Institute) ;
  • Choi, Jae-Won (ChungBuk Institute of Livestock and Veterinary Research) ;
  • Jeon, Soon-Hong (ChungBuk Institute of Livestock and Veterinary Research) ;
  • Kim, Kwan-Suk (Department of Animal Science, Chungbuk National University)
  • 하유경 (충북대학교 농업생명환경대학 축산학과) ;
  • 최정석 (충북대학교 농업생명환경대학 축산학과) ;
  • 김상욱 (충북대학교 농업생명환경대학 축산학과) ;
  • 최양일 (충북대학교 농업생명환경대학 축산학과) ;
  • 이승수 (국립축산과학원 개량평과과) ;
  • 최재원 (충청북도 축산위생연구소) ;
  • 전순홍 (충청북도 축산위생연구소) ;
  • 김관석 (충북대학교 농업생명환경대학 축산학과)
  • Received : 2009.05.08
  • Accepted : 2009.06.09
  • Published : 2009.06.01
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Abstract

The pork from black-coated pigs is famous among-consumers for better eating quality. The loci affecting black coat color was identified in pig chromosome 6 in which several genetic effects on pork quality have been reported. The melanocortin 1 receptor (MC1R) gene is a major gene which plays a key role in regulation of eumelanin (black/brown) and phaeomelanin (red/yellow). In this study, the MC1R gene polymorphism was analyzed for pig breed determination and genetic association with pork quality traits. MC1R Ala243Thr variation was analyzed to determine a specific genotype for four commercial pig breeds (Landrace, Yorkshire, Berkshire and, Duroc) and a Korean native pigs (KNP). Then we developed original KNP-specific DNA markers to determine the pork from black-coated pigs using MC1R DNA sequences. The total length of the MC1R coding sequence ranged 1451bp in KNP. KNP had the 0201 allele pertaining to $E^{D1}$ but some of the KNP had the $E^P$ allele, probably reflecting the geneticintrogression of $E^P$ allele into KNP. Furthermore, a relationship between Leu102Pro single nucleotide polymorphism (SNP) genotype and pork quality phenotype were analyzed in F2 reciprocal-crossbred population between KNP and Yorkshire. Association analysis indicated that the allele of the MC1R gene has no effect on pork quality. These results suggest that black coat-color is not directly associated with preferred pork quality, but the black-coat color pig breed may have other genetic components for superior pork quality.

본 연구는 한국재래돼지의 MC1R 유전자형을 결정하고 MC1R 유전자의 변이와 육질과의 연관성을 규명하기 위하여 실시하였다. 재래돼지의 MC1R 유전자형을 분석한 결과 중국재래돼지품종이 속하는 $E^{D1}$ 내에서도 0201형에 속하는 것으로 나타났으며 몇몇의 재래돼지에서는 $E^P$ 유전자형이 출현하였는데 이는 Berkshire 품종의 혼입으로 인한 것으로 사료된다. 흑색의 모색과 관련되어 있다고 보고된 Leu102Pro (T1132C) 변이를 재래돼지와 Yorkshire의 양방향 교배로 생산된 F2 집단의 171 두를 대상으로 분석하고 육질형질과의 연관성을 분석하였다. 모든 육질형질에 대하여 유의적인 효과를 나타내지 않았으며 이로써 돼지의 흑색모색과 육질과는 연관성이 없으며 다른 육질관련 유전자에 의해 영향을 받는다는 근거를 제공하였다. 또한 Duroc 품종에서 AA으로 고정되어 있어 다른 품종과의 구분 기준이 되는 Ala243Thr (G1554A) 변이를 이용하여 종돈검정소에서 출하된 Landrace, Yorkshire, Duroc, Berkshire 순종집단에 대한 유전자형 빈도를 조사하였으며 Berkshire를 제외한 나머지 품종에서 이형접합체가 일부 출현하였다.

Keywords

References

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