DOI QR코드

DOI QR Code

Effect of Irradiation on the Mixture of Egg White Proteins Responsible for Foaming Property

  • Liu, Xian De (Department of Animal Science and Biotechnology, Chungnam National University) ;
  • Han, Rong Xiu (Department of Animal Science and Biotechnology, Chungnam National University) ;
  • Jin, Dong-Il (Department of Animal Science and Biotechnology, Chungnam National University) ;
  • Lee, Soo-Kee (Department of Animal Science and Biotechnology, Chungnam National University) ;
  • Jo, Cheo-Run (Department of Animal Science and Biotechnology, Chungnam National University)
  • 투고 : 2009.10.07
  • 심사 : 2009.12.18
  • 발행 : 2009.12.01

초록

Irradiation of egg white increased foaming ability significantly. To investigate the protein modification by irradiation responsible for the increase of foaming ability, 3 major egg white proteins were purchased and mixed (7.7 g/L ovalbumin, 1.8 g/L ovotransferrin, 0.5 g/L lysozyme) as a model system and irradiated at 0, 2.5, and 5 kGy. The different protein expressions were evaluated using 2-D electrophoresis and it was found that ovotransferrin was cleaved by irradiation and molecular weight and isoelectric point were changed. In addition, many uncharacterized proteins were found and it indicated that irradiation modified proteins randomly but mainly fragmentation was observed. Therefore, it can be concluded that protein fragmentation of 3 major egg white proteins responsible for foaming ability may be the main reason for the improvement of foaming ability.

키워드

참고문헌

  1. Ball, H. R. and Gardner, F. A. 1968. Physical and functional properties of gamma irradiated liquid egg white. Poultry Sci. 47:1481-1487. https://doi.org/10.3382/ps.0471481
  2. Clark, D. C., Kiss, I. F., Wilde, P. J. and Wilson, D. R. 1992. The effect of irradiation on the functional properties of spray-dried egg white protein. Food Hydrocollids 5:541-548. https://doi.org/10.1016/S0268-005X(09)80123-9
  3. Damodaran, S. 2007. Amino acids, peptides, and proteins. Fenema’s Food Chemistry. 4th ed. Danadaran, S., Parkin, K. L. and Fenema, O. R. (ed.) CRC press, London, UK, pp. 269-281.
  4. Donovan, J. W., Mapes, C. J., Davis, J. G. and Garibaldi, J. A. 1975. A differntial scanning calorimetric study of the stability of egg white to heat denaturation. J. Sci. Food Agric. 26:73-83. https://doi.org/10.1002/jsfa.2740260109
  5. Filali-Mouhim, A., Audette, M., St-Louis, M., Thauvette, L., Denoroy, L., Penin, F., Chen, X., Rouleau, N., Le caer, J. P., Rossier, J., Potier, M. and Le Maire, M. 1997. Lysozyme fragmentation induced by $\gamma$-iradiolysis. Int. J. Radiat. Biol. 72:63-70. https://doi.org/10.1080/095530097143545
  6. Han, R. X., Kim, H. R., Naruse, K., Choi, S. M., Kim, B. C., Park, S. P. and Jin, D. I. 2007. Comparative study of protein profile during development of mouse placenta. Reprod. Dev. Biol. 31:253-269.
  7. Lechevalier, V., Croguennec, T., Pezennec, S., Guerin-Dublard, C., Pasco, M. and Nau, F. 2003. Ovalbumin, Ovotransferrin, Lysozyme: three model proteins for structural modifications at the air-water interface. J. Agric. Food Chem. 51:6354-6361. https://doi.org/10.1021/jf034184n
  8. Liu, X. D., Jang, A., Kim, D. H., Lee, B. D., Lee, M. and Jo, C. 2009. Effect of combination of chitosan coating and irradiation on physicochemical and functional properties of chicken egg during room temperature storage. Radiat. Phys. Chem. 78:589-591. https://doi.org/10.1016/j.radphyschem.2009.03.015
  9. Ma, C. Y., Sahasrabudhe, M. R., Poste, L. M., Harwalkar, V. R. and Chambers, J. R. 1990. Gamma irradiation of shell eggs. Internal and sensory quality, physicochemical characteristics, and functional properties. Canadian Inst. Food Sci. Technol. J. 23:226-232. https://doi.org/10.1016/S0315-5463(90)70248-9
  10. Marija, V., Marija B. R. and Bratoljub H. M. 2005 Gamma-radiation induced damage of proteins in the thick fraction of egg white. J. Serb. Chem. Soc. 11:1255-1262.
  11. Moon, S. and Song, K. B. 2001. Effect of gamma irradiation on the molecular properties of ovalbumin and ovomucoid and protection by ascorbic acid. Food Chem. 74:479-483. https://doi.org/10.1016/S0308-8146(01)00166-2
  12. Phillips, L. G., German, J. B., Foegeding, E. A., Harwalkar, A., Kilara, B. A., Lewins, B. A., Mangino, M. E., Morr, C. V., Regenstein, J. M., Smith, D. M., and Kinsella, J. E. 1990. Standardized procedure for measuring foaming properties of three proteins, a collaborative study. J. Food Sci. 55:1441-1444. https://doi.org/10.1111/j.1365-2621.1990.tb03953.x
  13. SAS. 2002. The SAS System for Windows (Release 9.1). SAS Institute Inc., Cary, NC.
  14. Schuessler, H. and Schilling, K. 1984. Oxygen effect in the radiolysis of proteins. Int. J. Radiat. Biol. 45:267-281. https://doi.org/10.1080/09553008414550381
  15. Song, H. P., Kim, B., Choe, J. H., Jung, S., Kim, K. S., Kim, D. H. and Jo, C. 2009. Improvement of foaming ability of egg white product by irradiation and its application. Radiat. Phys. Chem. 78:217-221. https://doi.org/10.1016/j.radphyschem.2008.10.001
  16. Van der Plancken, I., Van Loey, A. and Hendrickx, M. 2005. Change in sulfhydryl content of egg white proteins due to heat and pressure treatment. J. Agric. Food Chem. 53:5726-5733. https://doi.org/10.1021/jf050289+
  17. Wong, Y. C., Herald, T. J. and Hachmeister, K. A. 1996. Comparison between irradiated and thermally pasteurized liquid egg white on functional, physical, and microbiological properties. Poultry Sci. 75:803-808. https://doi.org/10.3382/ps.0750803

피인용 문헌

  1. Quality Prediction of Eggs Treated in Combination with Gamma Irradiation and Chitosan Coating Using Response Surface Methodology vol.53, pp.3, 2011, https://doi.org/10.5187/JAST.2011.53.3.253