YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Combination of Green Tea Extract and L-Theanine Alleviates Electric Foot Shock Induced Stress by Modulating Neurotransmitters in Mice

녹차추출물과 테아닌 복합물의 신경전달물질 조절을 통한 항스트레스 효과

  • Received : 2009.02.03
  • Accepted : 2009.05.14
  • Published : 2009.10.31
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Abstract

Various neurotransmitters are involved in regulating stress systems. In this study, we investigated the combination relieving effect of green tea extract(GTE) and L-theanine on the stress induced by electric foot shock. Four week oral administration of GTE (24 mg/kg), L-theanine (4 mg/kg) or their combination reduced the levels of dopamine, noradrenaline and corticosterone in blood, brain cortex, hippocampus, and striatum, wherease increased serotonin level. The combination of GTE and L-theanine showed much greater effects than single treatment of each component, and the effects are comparable to diazepam (2 mg/kg). Therefore, this study suggests that the combination of GTE and L-theanine may act effective and be useful for stress relieving treatment.

Keywords

References

  1. Charney, D. S. : Neuroanatomical circuits moculating fear and anxiety behaviors. Acta. Psychiatr. Scand. Suppl. 417, 38 (2003)
  2. Millan, M. J. : The neurobiology and control of anxious states. Prog. Neurobiol. 70, 83 (2003) https://doi.org/10.1016/S0301-0082(03)00087-X
  3. Sugimoto, Y., Furutani, S., Itoh, A., Nakajima, H., Oshiro, H., Sun, S. and Yamada, J. : Effects of extracts and neferine from the embryo of Nelumbo nucifera seeds on the central nervous system. Phytomedicin. 15, 1117 (2008) https://doi.org/10.1016/j.phymed.2008.09.005
  4. Arago, G. F., Carneiro, L. M., Junior, A. P., Vieira, L. C., Bandeira, P. N., Lemos, T. L. and Viana, G. S. : A possible mechanism for anxiolytic and antidepressant effects of alphaand beta-amyrin from Protium heptaphyllum (Aubl.) March. Pharmacol. Biochem. Behav. 85, 827 (2006) https://doi.org/10.1016/j.pbb.2006.11.019
  5. L-theanine : monograph, Alter Med Rev. 10, 136 (2005)
  6. 윤상원 : Sun-$\alpha$가 함유된 음료수가 뇌파출현 및 암기력에 미치는 영향에 관한 분석 연구. 한국정신과학회지 1, 109 (1997)
  7. Kalivas, P. W. and Duffy, P. : Selective activation of dopamine transmission in the shell of the nucleus accumbens by stress. Brain Res. 675, 325 (1995) https://doi.org/10.1016/0006-8993(95)00013-G
  8. Tidey, J. W. and Miczek, K. A. : Social defeat stress selectively alters mesocorticolimbic dopamine release: an in vivo microdialysis study. Brain Res. 721, 140 (1996) https://doi.org/10.1016/0006-8993(96)00159-X
  9. Gesto, M., Soengas, J. L. and Miguez, J. M. : Acute and prolonged stress responses of brain monoaminergic activity and plasma cortisol levels in rainbow trout are modified by PAHs (naphthalene, beta-naphthoflavone and benzo(a)pyrene) treatment. Aquat. Toxicol. 86, 341 (2008) https://doi.org/10.1016/j.aquatox.2007.11.014
  10. Buffalari, D. M. and Grace, A. A. : Chronic cold stress increases excitatory effects of norepinephrine on spontaneous and evoked activity of basolateral amygdala neurons. Int. J. Neuropsychopharmacol. 12, 95 (2009) https://doi.org/10.1017/S1461145708009140
  11. Saito, T., Ishiwata, T., Hasegawa, H., Nomoto, S., Kotani, Y., Otokawa, M. and Aihara, Y. : Effect of chronic cold exposure on noradrenergic modulation in the preoptic area of thermoreulation in freely moving rats. Life Sci. 83, 79 (2008) https://doi.org/10.1016/j.lfs.2008.05.008
  12. Lapiz-bluhm, M. D., Soto-pina, A. E., Hensler, J. G. and Morilak, D. A. : Chronic intermittent cold stress and serotonin depletion induce deficits of reversal learning in an attentional set-shifting test in rats. Psychopharmacology (Berl) 202, 329 (2009) https://doi.org/10.1007/s00213-008-1224-6
  13. Parker, C. C., Ponicsan, H., Spencer, R. L., Holmes, A. and Johnson, T. E. : Restraint stress and exogenous corticosterone differentially alter sensitivity to the sedative-hypnotic effects of ethanol in inbred long-sleep and inbred short-sleep mice. Alcohol. 42, 477 (2008) https://doi.org/10.1016/j.alcohol.2008.05.004
  14. Swiergiel, A. H., Li, Y., Wei, Z. Y. and Dunn, A. J. : Effects of chlordiazepoxide on footshock- and corticotropin-releasing factor-induced increases in cortical and hypothalamic norepinephrine secretion in rats. Neurochem. Int. 52, 1220 (2008) https://doi.org/10.1016/j.neuint.2008.01.002
  15. Komiya, M., Takeuchi, T. and Harada, E. : Lemon oil vapor causes an anti-stress effect via modulating the 5-HT and DA activities in mice. Behav. Brain Res. 17. 172, 240 (2006) https://doi.org/10.1016/j.bbr.2006.05.006
  16. Seo, J., Lee, S., Lee, Y., Kwon, B., Ma, Y., Hwang, B., Hong, J. and Oh, K. : Anxiolytic -like effects of obovatol isolated from Magnolia obovata:Involvement of GABA/benzodiazepine receptors complex. Progress in Neuro-Psychopharmacology & Biological Psychiatry 31, 1363 (2007) https://doi.org/10.1016/j.pnpbp.2007.05.009
  17. Yamada, T., Terashima, T., Okubo, T., Juneja, L. R. and Tokogoshi, H. : Effects of theanine, gamma-glutamylethylamide, on neurotransmitter release and its relationship with glutamic acid neurotransmission. Nutr. Neurosci. 8, 219 (2005) https://doi.org/10.1080/10284150500170799
  18. Yokogoshi, H., Kobayashi, M., Mochizuki, M. and Terashima, T. : Effect of theanine, r-glutamylethylamide, on brain monoamines and striatal dopamine release in conscious rats. Neurochem. Res. 23, 667 (1998) https://doi.org/10.1023/A:1022490806093