Asian Journal of Turfgrass Science (아시안잔디학회지)

Turfgrass Society of Korea (한국잔디학회)
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Development of SCAR markers in Creeping bentgrass(Agrostis palustrics Huds.) cultivars

Creeping bentgrass(Agrostis palustrics Huds.) 품종별 SCAR markers 개발

  • Received : 2009.09.01
  • Accepted : 2009.11.22
  • Published : 2009.12.31
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Abstract

Creeping bentgrass (Agrostis palustrics Huds.) is cool season turfgrasse that is used for putting green in golf course. Creeping bentgrass cultivars are difficult to distinguish with the same species because of similar morphological characters and low level of genetic diversity. The SCAR markers using the specific DNA can be useful for differentiating between creeping bentgrass cultivars. Five RAPD primers were used for specific band detection among creeping bentgrass cultivars, penncross, penn A-4, crenshaw, L-93, CY-2, T-1. The pairs of SCAR primers for six cultivers were designed by the specific sequences of the bands that amplified by RAPD. Three of the six SCAR primers could not make the use as SCAR primers because the specific false bands were detected in all cultivars. The remaining pairs of SCAR primer, CY850F/R, T700F/R, L2900F/R, amplified the specific band at expected size for three cultivars, CY-2, T-1, L-93, respectively. The CY850F/R primer amplified a band of 850bp in CY-2 cultivar, the T700F/R primer amplified a band of 700bp in T-1 cultivar, and the L2900F/R primer amplified a band of 2.9kb in L-93 cultivar. In this study we developed the SCAR markers to identify and distinguish the inerseeded creeping bentgrass cultivars in a golf course green.

크리핑벤트그래스는 골프코스의 퍼팅그린에서 주로 사용하고 있는 한지형잔디 초종이다. 크리핑벤트그래스 품종들은 형태적인 특성이나 유전적인 다양성이 협소하여 품종간 구분이 매우 어렵다. 따라서 이들 품종간 유전적인 다양성이나 골프코스 그린의 인터씨딩율에 대한 조사를 위해 SCAR marker를 개발하고자 본 연구를 수행하였다. penncross, penn A-4, crenshaw, L-93, CY-2, T-1 공시품종들에 대한 SCAR marker 개발을 위해 5개 RAPD primer에 대한 품종 간 구분이 가능한 특이적 band를 선발하였다. 선발한 특이적 band의 염기서열을 분석하여 품종별 SCAR primer를 제작하였다. 각 품종의 SCAR primer별로 특이적 band가 나타나는지에 대한 여부를 검정한 결과, penncross, penn A-4, crenshaw 품종의 SCAR primer는 6개 공시품종에서 동일한 크기의 band가 나타나 SCAR marker로써 활용이 어려웠다. 하지만 CY-2의 CY850F/R primer는 850bp, T-1의 T700F/R primer는 700bp, L-93의 L2900F/R는 2.9kb에서 특이적 band가 나타나 3개 품종별 SCAR marker로서 활용이 가능하였다. 따라서 본 연구에서 개발한 3개 품종별 SCAR marker를 활용하여 크리핑벤트그래스의 품종별 유전적 다양 및 골프코스 그린의 인터씨딩율 조사에 활용이 가능할 것으로 기대된다.

Keywords

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