The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Neuroprotective Effects of Bunsimgieum

분심기음(分心氣飮)의 도파민 세포 보호 효과

  • Kim, Ro-Sa (Dept. of Gynecology, College of Oriental Medicine, KyungHee University) ;
  • Lee, Chang-Hoon (Dept. of Gynecology, College of Oriental Medicine, KyungHee University) ;
  • Lee, Jin-Moo (Dept. of Gynecology, College of Oriental Medicine, KyungHee University) ;
  • Cho, Jung-Hoon (Dept. of Gynecology, College of Oriental Medicine, KyungHee University) ;
  • Jang, Jun-Bock (Dept. of Gynecology, College of Oriental Medicine, KyungHee University) ;
  • Lee, Kyung-Sub (Dept. of Gynecology, College of Oriental Medicine, KyungHee University)
  • 김로사 (경희대학교 한의과대학 한방부인과학 교실) ;
  • 이창훈 (경희대학교 한의과대학 한방부인과학 교실) ;
  • 이진무 (경희대학교 한의과대학 한방부인과학 교실) ;
  • 조정훈 (경희대학교 한의과대학 한방부인과학 교실) ;
  • 장준복 (경희대학교 한의과대학 한방부인과학 교실) ;
  • 이경섭 (경희대학교 한의과대학 한방부인과학 교실)
  • Published : 2009.05.29
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Abstract

Purpose: The depression accompanied with menopuase shows the relation with the dopamine secretion. These studies were undertaken to evaluate the anti- oxidative and neuroprotective effects of Bunsimgieum(BSGE) on dopaminergic neurons. Methods: To estimate the antioxidant effects, we carried out 1.1-diphenyl-2- picrylhydrazyl (DPPH) free radical scavenging assay, 2,2'-azinobis-(3-ethylbenzothiazoline -6-sulfonic acid (ABTS) radical cation decolorization assay, and measurement of total polyphenolic content. To evaluate neuroprotective effect of BSGE in vitro, We performed thiazolyl blue tetrazolium bromide (MTT) assay, reactive oxygen species (ROS) creation in SH-SY5Y. Tyrosine hydroxylase (TH) immunocytochemistry, nitric oxide (NO) assay, and TNF-${\alpha}$ assay in primary rat mesencephalic dopaminergic neurons. Results: The DPPH free radical and the ABTS radical cation inhibition activities were increased at a dose dependent manner. Total polyphenolic content was 0.83%. In SH-SY5Y culture, BSGE significantly increased the decreased cell viability by 6-OHDA at the concentrations of 10${\mu}$g/m${\ell}$ in pre-treatment group, 0.1-200${\mu}$g/m${\ell}$ in post-treatment group. The production of ROS induced by 6-OHDA was significantly inhibited in BSGE treated group. In mesencephalic dopaminergic cell culture, the BSGE group reduced the dopaminergic cell loss against 6-OHDA toxicity and the production of No and TNF-${\alpha}$ at the concentration of 5${\mu}$g/m${\ell}$. Conclusion: These results shows that BSGE has antioxidant and neuroprotective effects in the dopaminergic cells through decreasing the production of ROS, NO and TNF-${\alpha}$ which can cause many neurodegenerative changes in brain cell. We suggest that BSGE could be useful for the treatment of postmenopausal depression related with the decrease of dopamine.

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References

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