The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
권호 표지
DOI QR코드

DOI QR Code

The Experimental Study on Anti-inflammatory Effect of Manbunbang

만분방(慢盆方)이 염증 관련 cytokines의 유전자 발현과 생성량에 미치는 영향

  • Kwak, Sang-Ho (Dept. of Gynecology, College of Oriental Medicine, Daejeon University) ;
  • Yoo, Dong-Youl (Dept. of Gynecology, College of Oriental Medicine, Daejeon University)
  • 곽상호 (대전대학교 한의과대학 부인과교실) ;
  • 유동열 (대전대학교 한의과대학 부인과교실)
  • Published : 2009.05.29
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: This study was performed to evaluate the anti-inflammatory effect of Manbunbang extract (MBB). Methods: In order to understand the mechanism of anti-inflammatory effect of MBB, expression of cytokines and its levels in RAW 264.7 cell lines, as well as changes of cytokine gene expressions in serum, spleen, and liver tissues in acute inflammation induced mouse model were investigated. Results: 1. MBB significantly suppressed the expression levels of IL-1${\beta}$, TNF-${\alpha}$ and COX-2 mRNAs at 100 and 50 ${\mu}$g/m${\ell}$ concentrations, and IL-6 and NOS-II genes at 100, 50 and 10 ${\mu}$g/m${\ell}$ concentrations in RAW 264.7 cell lines, compared to those of the control. 2. MBB significantly reduced the production level of IL-$1{\beta}$, IL-6 and TNF-${\alpha}$ at 100 and 50 ${\mu}$g/m${\ell}$ concentrations in RAW 264.7 cell lines compared the those of the control. 3. MBB significantly reduced the production of IL-1${\beta}$, IL-6 and TNF-${\alpha}$ levels in sera of acute inflammation induced mice. 4. MBB significanlty suppressed the expression level of IL-1${\beta}$, TNF-${\alpha}$ mRNA in spleen tissues as well as IL-6 mRNA in liver tissues in acute inflammation induced mice. Conclusion: From the results above, anti-inflammatory effect of MBB through its immune regulation could be experimentally explained. Wide treatment of inflammatory diseases such as pelvic inflammation using MBB are recommended.

Keywords

References

  1. 대한산부인과학회. 부인과학. 부산: 현대의학서적사. 1987;569-588.
  2. 韓國産婦人科學會 敎科書編纂委員會. 婦人科學. 서울: 도서출판 칼빈서적. 1997;256-283.
  3. 한의부인과학 편찬위원회. 한의부인과학(상). 서울: 정담출판사. 2002;266-281.
  4. 李秀芝. 骨盤炎 1, 2號方治療骨盤炎70 例. 山東中醫雜誌. 1993;12(1):21.
  5. 朱小南 등. 女科經驗選. 北京: 人民衛生出版社. 2006;132-134.
  6. 夏桂成. 中醫臨床婦科學. 北京: 人民 衛生出版社. 1994;285-293.
  7. 牛建昭. 現代中西婦科學. 北京: 中國 科學技術出版社. 1996;230-260.
  8. 陳自明. 婦人良方大全. 北京: 文光圖書有限公司. 1982;7:34.
  9. 羅元恺 등. 實用中醫婦科學. 上海: 上海科學技術出版社. 1994;255-259.
  10. 제종민. 稜莪消積湯의 항혈전 및 항염작용에 대한 실험적 연구. 대전대학교 대학원. 박사학위논문. 2007.
  11. 임동욱 등. 複方紅藤敗醬散의 항혈전 및 항염작용에 대한 실험적 연구. 대한한방부인과학회지. 2006;19(3):151-174.
  12. 이혜경. 加味通經湯의 항혈전, 소염 및 진통작용에 대한 연구. 대한한방부인과학회지. 2003;16(1):118-135.
  13. 이상수 등. 少腹逐瘀湯이 항혈전 및 소염, 진통에 미치는 영향. 대한한방부인과학회지. 1996;9(1):81-96.
  14. 정우석. 慢盆方이 혈전 병태 유관 인자에 미치는 영향. 원광대학교 대학원. 박사학위논문. 2007.
  15. Young JL, Libby P, Schonbeck U. Cytokines in the pathogenesis of atherosclerosis. Thromb Haemost. 2002;88:554-567.
  16. Wakefield TW, Henke PK. The role of inflammation in early and late venous thrombosis: Are there clinical implications?. Semin Vasc Surg. 2005 ;18(3):118-129. https://doi.org/10.1053/j.semvascsurg.2005.05.003
  17. Fox EA, Kahn SR. The relationship between inflammation and venous thrombosis. A systematic review of clinical studies. Thromb Haemost. 2005;94(2):362-365.
  18. Jeffrey F et al. Clinical predictors of endometritis in women with symptoms and signs of pelvic inflammatory disease. American Journal of Obstetrics and Gynecology. April. 2001;184(5) :856-864. https://doi.org/10.1067/mob.2001.113847
  19. 蕭塤. 女科經綸. 江蘇省: 江蘇科學技術出版社. 1986;40.
  20. 조수인. 흰쥐 신장 조직 손상에 대한 熟地黃의 항산화 효과. 대한본초학회지. 2003;18(4):119-126.
  21. 이용범 등. 식이성 고지혈증에 미치는 숙지황의 영향 및 항산화 활성에 관한 연구. 環境硏報. 2001;11(1):41-57.
  22. 부용출 등. 녹차와 목단피의 항산화 성분. 韓國農化學會誌. 1993;36(5):326-331.
  23. 정일윤 등. 백작약 열수 추출물의 산화적 스트레스 억제효과 및 유효 성분 동정. 한국식품영양과학회지. 2003;32(5):739-744.
  24. 최수임 등. 생약재 추출물의 hyaluronidase 저해 및 라디칼 소거 활성검색. 한국생물공학회지. 2003;18(4):282-288.
  25. 김세종. 면역학길라잡이. 서울: 고려의학. 2000;65-68.
  26. 홍천수 역. Pathophysiology로 이해하는 내과학. 서울: 도서출판 정담. 2002;10-12.
  27. Aeberi D et al. Inhibition of the TNF-pathway use of infliximab and etanercept as remission-inducing agents in case of therapy-resistant chronic inflammatory disorders. Swiss Med. Wkly. 2002;132:414-422.
  28. Feldman M et al. Anti-TNF-$\alpha$ therapy is useful in rheumatoid arthritis and Crohn's disease: analysis of the mechanism of action predicts utility in other disease. Transplant Proc. 1998;30:4126-4127. https://doi.org/10.1016/S0041-1345(98)01365-7
  29. Brian TP et al. The effect of fetal acidemia on fetal-placental vascular tone and production of inflammatory cytokines interleukin-6 and tumor necrosis factor-$\alpha$. American Journal of Obstetrics and Gynecology. 2002 ;187(4):894-897. https://doi.org/10.1067/mob.2002.127455
  30. Henrik Dollner. Histologic chorioamnionitis and umbilical serum levels of procytokines and cytokine inhibitors. An International Journal of Obstetrics and Gynecology. 2002;109:534-539. https://doi.org/10.1111/j.1471-0528.2002.01028.x
  31. Matthias Winkler. Role of cytokines and other inflammatory mediators. An International Journal of Obstetrics and Gynecology. 2003;110:118-123. https://doi.org/10.1046/j.1471-0528.2003.00062.x
  32. Charles N et al. Expression of cyclooxygenase-2 in cervical, endometrial, and ovarian malignancies. American Journal of Obstetrics and Gynecology. 2003;188(5):1174-1176. https://doi.org/10.1067/mob.2003.284
  33. 지병철, 문신용. Nitric Oxide가 인간황체화 과립막세포의 아프토시스에 미치는 영향. 대한산부인과학회지. 2002;45(5):766-773.
  34. Carla B et al. Interactions between the nitric oxide and prostaglandin E2 biosynthetic pathways in human amnion-like WISH cells. Journal of Reproductive Immunology. 2003;60(1):35-52. https://doi.org/10.1016/S0165-0378(03)00080-9