The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Effects of Woo-Gui-Um on A${\beta}$ Toxicity and Memory Dysfunction in Mice

  • Hwang, Gwang-Ho (Graduate School of East-West Medicine, Kyung Hee University) ;
  • Kim, Bum-Hoi (College of Oriental Medicine, Kyung Hee University) ;
  • Shin, Jung-Won (Department of Anatomy & Neurobiology, School of Medicine, Washington University) ;
  • Shim, Eun-Sheb (College of Oriental Medicine, Kyung Hee University) ;
  • Lee, Dong-Eun (Graduate School of East-West Medicine, Kyung Hee University) ;
  • Lee, Sang-Yul (College of Oriental Medicine, Kyung Hee University) ;
  • Lee, Hyun-Sam (College of Oriental Medicine, Kyung Hee University) ;
  • Jung, Hyuk-Sang (College of Oriental Medicine, Kyung Hee University) ;
  • Sohn, Nak-Won (Graduate School of East-West Medicine, Kyung Hee University) ;
  • Sohn, Young-Joo (Department of Gynecology, College of Oriental Medicine, Sangji University)
  • Published : 2009.05.30
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Abstract

Objectives : Alzheimer's disease (AD) is characterized by neuronal loss and extracellular senile plaque. Moreover, the cellular actions of ${\beta}$-amyloid (A${\beta}$ play a causative role in the pathogenesis of AD. This study was designed to determine whether Woo-Gui-Um, a commonly used Korean herbal medicine, has the ability to protect cortical and hippocampal neurons against A${\beta}_{25-35}$ neurotoxicity Methods : In the present study, the authors investigated the preventative effects of the water extract of Woo-Gui-Um in a mouse model of AD. Memory impairment was induced by intraventricularly (i.c.v.) injecting A${\beta}_{25-35}$ peptides into mice. Woo-Gui-Um extract was then administered orally (p.o.) for 14 days. In addition, A${\beta}_{25-35}$ toxicity on the hippocampus was assessed immunohistochemically, by staining for Tau, MAP2, TUNEL, and Bax, and by performing an in vitro study in PC12 cells. Results : Woo-Gui-Um extract had an effect to improve learning ability and memory score in the water maze task. Woo-Gui-Um extract had significant neuroprotective effects in vivo against oxidative damage and apoptotic cell death of hippocampal neurons caused by i.c.v. A${\beta}_{25-35}$. In addition, Woo-Gui-Um extract was found to have a protective effect on A${\beta}_{25-35}$-induced apoptosis, and to promote neurite outgrowth of nerve growth factor (NGF)-differentiated PC12 cells. Conclusions : These results suggest that Woo-Gui-Um extract reduces memory impairment and Alzheimer's dementia via an anti-apoptotic effect and by regulating Tau and MAP2 in the hippocampus.

Keywords

References

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