Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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The Anticancer Effect of Inonotus obliquus Pilat (Chaga) Processed by Nanomill Technology In vivo

나노밀 가공된 차가버섯의 항암효과

  • Kim, Dong-Heui (Department of Environmental Medical Biology, Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University) ;
  • Teng, Yung-Chien (Department of Environmental Medical Biology, Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University) ;
  • Yoon, Yang-Sook (Department of Environmental Medical Biology, Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University) ;
  • Qi, Xu-Feng (Department of Environmental Medical Biology, Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University) ;
  • Jeong, Hyun-Seok (Leejema Ltd., Hongcheon Life Health Research Center) ;
  • Chang, Byung-Soo (Department of Cosmetology, Hanseo University) ;
  • Lee, Kyu-Jae (Department of Environmental Medical Biology, Institute of Lifelong Health, Wonju College of Medicine, Yonsei University)
  • 김동희 (연세대학교 원주의과대학 환경의생물학교실, 기초의학연구소) ;
  • 등영건 (연세대학교 원주의과대학 환경의생물학교실, 기초의학연구소) ;
  • 윤양숙 (연세대학교 원주의과대학 환경의생물학교실, 기초의학연구소) ;
  • 최주봉 (연세대학교 원주의과대학 환경의생물학교실, 기초의학연구소) ;
  • 정현석 (주식회사 이제마) ;
  • 장병수 (한서대학교 보건학부 피부미용학과) ;
  • 이규재 (연세대학교 원주의과대학 환경의생물학교실, 평생건강사업단)
  • Received : 2009.04.16
  • Accepted : 2009.06.20
  • Published : 2009.06.30
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Abstract

Extracts and fractions of Inonotus obliquus (Chaga in Russia) have been known to have various biological activities, including antimutagenic, anticancer, antioxidative, and immunostimulating effects. This study was performed to confirm anticancer effect of 10% superfine Chaga mushroom processed by nano-mill technology on C57BL/6 mice. Chaga particles belonged in the size of 1 ${\mu}m$ was about 40% after nanomill processing according to the volume distribution. As the result of subcutaneous injection of B16BL6 melanoma cells to the mice, the tumor volume (p<0.001) and tumor weight (p<0.01) was significantly decreased in the experimental (NCh) group as compared with control (C) group and the tumor growth inhibitory rate was 29.2%. On examination of survival rate after intraperitoneal injection of B16BL6 melanoma cells, the mean survival time per a mouse was 17.7 and 26.0 days in C and NCh group respectively. The survival rate of NCh group was 40% when that of C group was 0% at the 35th day. On the result of examination to confirm histological toxicity by Chaga superfine particles, both groups did not show any morphological and pathological changes in the small and large intestine under the light microscope. These results suggest that feeding of superfine Chaga produced by nanomill technique has a tumor growth inhibitory effect in vivo.

차가버섯(Inonotus obliquus)의 추출물과 분획들은 항돌연변이, 항암, 항산화 및 면역자극 효과 등을 비롯하여 다양한 생물학적 활성을 갖는 것으로 알려져 있다. 본 연구는 나노분쇄 기법으로 제작된 10% 차가사료를 C57BL/6 생쥐에게 식이한 후 항암효과를 확인하기 위해 시행되었다. 부피평균에 의한 입도분석에 따르면 1 ${\mu}m$ 내에 포함되는 초미세 차가버섯 입자는 전체의 40%인 것으로 나타났다. 생쥐에게 B16BL6 흑색종세포를 피하로 주사한 결과, 종양의 부피(p<0.001)와 무게(p<0.01)는 초미세 차가버섯을 식이한 실험군(Nch)이 일반사료를 먹인 대조군(C)에 비해 현저히 감소하였고, 종양성장 억제율은 29.2%를 나타내었다. 흑색종 세포를 복강 내로 주사한 후 생존율을 관찰한 결과, 마리 당 평균 생존기간은 대조군과 실험군이 각각 17.7일과 26.0일이었다. 대조군이 모두 사망한 날인 35일 째 실험군의 생존율은 40%를 보여주었다. 초미세 차가입자가 소화기관에 문제를 유발시킬 수 있는 가능성 때문에 안전성을 확인하기 위해 소장과 대장을 조직학적으로 관찰한 결과 두 군 모두 형태학적 또는 병리학적 변화는 관찰되지 않았다.

Keywords

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