Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
권호 표지

Populations of Rod and Cone Photoreceptors in the Hamster Retina

햄스터 망막에서의 광수용체 분포

  • Yu, Song-Hee (Department of Biology, College of Natural Sciences, and Brain Science and Engineering Institute, Kyungpook National University) ;
  • Kim, Hyun-Jin (Department of Biology, College of Natural Sciences, and Brain Science and Engineering Institute, Kyungpook National University) ;
  • Lee, Kyoung-Pil (Department of Biology, College of Natural Sciences, and Brain Science and Engineering Institute, Kyungpook National University) ;
  • Lee, Eun-Shil (Department of Biology, College of Natural Sciences, and Brain Science and Engineering Institute, Kyungpook National University) ;
  • Lee, Jea-Young (Department of Biology, College of Natural Sciences, and Brain Science and Engineering Institute, Kyungpook National University) ;
  • Jeon, Chang-Jin (Department of Biology, College of Natural Sciences, and Brain Science and Engineering Institute, Kyungpook National University)
  • 유성희 (경북대학교 자연과학대학 생물학과, 생명과학 및 뇌 과학 연구소) ;
  • 김현진 (경북대학교 자연과학대학 생물학과, 생명과학 및 뇌 과학 연구소) ;
  • 이경필 (경북대학교 자연과학대학 생물학과, 생명과학 및 뇌 과학 연구소) ;
  • 이은실 (경북대학교 자연과학대학 생물학과, 생명과학 및 뇌 과학 연구소) ;
  • 이제영 (경북대학교 자연과학대학 생물학과, 생명과학 및 뇌 과학 연구소) ;
  • 전창진 (경북대학교 자연과학대학 생물학과, 생명과학 및 뇌 과학 연구소)
  • Received : 2009.09.23
  • Accepted : 2009.12.24
  • Published : 2009.12.31
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Abstract

We report on a quantitative analysis of cone and rod photoreceptors in hamster retina. Cone and rod photoreceptors were counted in retinal whole mounts using differential interference contrast (DIC) optics microscopy after staining of cone photoreceptors were stained with peroxidase-labeled peanut lectin. Middle-to-long-wave-sensitive-(M/L-), and shortwave-sensitive-(S-) cone opsins were visualized by observed using confocal microscope after immunocytochemical procedure. The average cone density was 9,307 $cells/mm^2$, giving a total of cones of 293,060 cone cells per retina. The peak density of cone cells (12,857 $cells/mm^2$) was found 0.3 mm from the optic disk (OD) of the nasal retina. The average rod density was 300,082 $cells/mm^2$, giving a total number of rods of 9,448,150 cells. The peak density of rod cells was found 0.3 mm from the OD of the dorsal retina. Of all photoreceptors studied, the total percentage of rods and cones were 96.99% and cones 3.01%, respectively. The mean ratio of rod and cone was 32.24 : 1. The cone photoreceptors of hamster contained both M/L- and S-cone opsins. The present results suggest that the hamster retina is strongly rod-dominated with some photopic property of vision.

본 연구에서는 햄스터 망막의 추상과 간상 광수용체의 양적 분석을 하였다. 추상 광수용체를 과산화 효소로 표지된 피넛 렉틴(peroxidase-labeled peanut lectin)을 사용하여 염색한 뒤 광학 미분간섭 현미경(DIC, differential interference contrast optics)을 사용하여 관찰하였다. 또한 면역세포화학적 방법으로 추상 광수용체에 위치한 M/L-옵신과 S-옵신을 염색한 뒤 공초점 형광 현미경(confocal microscope)을 사용하여 관찰하였다. 추상세포의 평균 밀도는 9,307 $cells/mm^2$이며 전체 추상세포는 293,060개였다. 추상세포의 최대값(12,857 $cells/mm^2$)은 맹점(시신경 유두, optic disk)로부터 코 쪽 망막(nasal retina)으로 0.3 mm 떨어진 곳에 위치하였다. 간상세포의 평균은 300,082 $cells/mm^2$으로 전체 간상세포는 9,448,150개였다. 간상세포의 최대값(340,000 $cells/mm^2$)은 맹점으로부터 등쪽 망막(dorsal retina) 방향으로 0.3 mm 떨어진 곳에서 발견되었다. 평균적으로 전체 광수용체 중, 간상세포의 전체 분포는 96.99%이고 추상세포는 3.01%를 차지하였다. 또한 간상과 추상의 평균 비는 32.24 : 1이었다. 햄스터의 추상세포는 M/L-와 S-옵신을 가진다. 본 연구 결과는 햄스터가 일부 명소시를 가지는 강한 간상 우세 망막임을 제시한다.

Keywords

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