Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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The Whitening Effect of Angelicae dahuricae Radix Water Extract in Brown Guinea Pigs

Brown guinea pig에서 백지 열수추출물의 미백효과

  • 김필순 (계명대학교 대학원 공중보건학) ;
  • 김영철 (계명대학교 대학원 공중보건학)
  • Received : 2009.09.05
  • Accepted : 2009.10.19
  • Published : 2009.10.31
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Abstract

The purpose of this study was to investigate the whitening effect of Angelicae dahuricae Radix water extract (ADWE) by its application on artificial tanning spots which were induced by 1,500 $mJ/cm^2$ of UVB radiation on the backs of brown guinea pigs weighing approximately 450~500 g. Thirty ${\mu}l$ of ADWE, at each application, were applied twice a day, 5 days a week, for 8 weeks to the guinea pigs. The artificial tanning spots were divided into 3 groups which were vehicle control group [propylene glycol: ethanol: water (5:3:2)], positive control group (2% hydroquinone) and experimental group (2% ADWE). The visible whitening effect and changes in melanin index were evaluated once a week. On completion of the experiment, the animals were sacrificed under anesthetization, and the artificial tanning spots were excised by biopsy punch and stained with Hematoxylin and Eosin (H&E) to observe histological change and also stained with Fontana-Masson's silver (F-M) and S-100 to observe melanin pigmentation and melanocytes. In the gross observation, the experimental group showed higher pigmentation than the positive control group but lower pigmentation than the vehicle control group. Histological observation confirmed that ADWE had a positive whitening effect by showing a lower distribution of melanin and melanocytes in the epidermis of experimental group than in the vehicle control group.

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References

  1. Choi, W. H., Ann, H. S., Choi, T. Y., Jin, S. Y., Ahn, R.M. : Effects of natura extracts on UVB-induced pigmentation and inflammation in C57BL/6 mouse skin. Korean Journal of Environmental Health Science, 32(5), 492-498, 2006
  2. Lee, M., Yi, S. Y., Ahn, H. S., Ahn, R. M. : Lipid peroxidation and antioxidase activities on Hairless mouse induced by UVB irradiation and effects of tannic acid. Korean Journal of Environmental Health Science, 22(4), 102-108, 1996
  3. Park, S. N. : Skin aging and oxygen species. Journal of Cosmetic Science, 23(1), 75-132, 1997
  4. Lee, E. H., Lee, J. K., Hong, J. T., Jung, K. M., Kim, Y. K., Lee, S. E., Chung, S. Y., Lee, Y. W. : Protective effect Green Tea extract catechin on UVBinduced skin damage. Journal of Food Hygiene and Safety, 16(2), 117-124, 2001
  5. Park, Y. I. : The effect of modified yeonryeonggobondan on skin care (Focused on anti-oxidant, reducing melanogenesis, anti-inflammation and anti-bacteria).Dept. of Oriental Medicine, Graduate School of Sangji University, 2006
  6. Briganti, S., Camera, E., Picardo, M. : Chemical and instrumental approaches to treat hyperpigmentation. Pigment Cell Research, 16(2), 101-110, 2003 https://doi.org/10.1034/j.1600-0749.2003.00029.x
  7. Agar, N. and Young, A. R. : Melanogenesis : a photoprotective response to DNA damage? Mutation Research, 571(1), 121-132. 2005 https://doi.org/10.1016/j.mrfmmm.2004.11.016
  8. Maeda, K., Fukuda, M. : In vitro effectiveness of several whitening cosmetic components in human melanocytes. Journal of the Society Cosmetic Chemists, 42(6), 361-368, 1991
  9. Schrode, J. : C y tokine n etworks in t he s kin. Journal of Investigative Dermatology, 105(1), 20-24, 1995 https://doi.org/10.1038/jid.1995.5
  10. Jang, H. K., Roh, S. S. : Depigmentation activity of many herb. Dept. of Oriental Medical Ophthalmology & Otolaryngology, College of Oriental Medicine, Daejeon University, 13(2), 289-302, 2004
  11. Kobayashi, T., Imokawa, G., Bennett, D. C., Hearing, V. J. : Tyrosinase stabilization by Tyrpl(the brown locus protein). Journal of Biological Chemistry, 273(48), 3180-3185, 1998 https://doi.org/10.1074/jbc.273.6.3180
  12. Terasaka, H., Takayama, F., Satoh, K., Fujisawa, S., Sakagami, H. : Effect of antioxidants on radical intensity and cytotoxicity of hydroquinone. Anticancer Research, 20(5), 3357-3362, 2000
  13. Cabanes, J., Chazarra, S., Garcia-Carmona, F. : Kojic acid, a cosmetic skin whitening agent, is a show binding inhibitor of cathecilase activity of tyrosinase. Journal of Pharmacology, 46(12), 982-985, 1994 https://doi.org/10.1111/j.2042-7158.1994.tb03253.x
  14. Sugimoto, K., Nishhura, T., Nomura, K., Kuriki, T. :Inhibitory effects of alpha arbutin on melanin synthesis in cultured human melanoma cells and a three-dimensional human skin model. Biological & Pharmaceutical Bulletin, 27(4), 510-514, 2004 https://doi.org/10.1248/bpb.27.510
  15. Aburjai, T., Natsheh, F. M. : Plants used in cosmetics. Phytotherapy Research, 17(9), 987-1000, 2003 https://doi.org/10.1002/ptr.1363
  16. Kim, S. H., Kang, S. S., Kim. C. M. : Coumarin glycosides from the roots of Angelicae dahuricae. Archives of Pharmacological Research, 15(1), 73-77. 1992 https://doi.org/10.1007/BF02973988
  17. Pae H. O., Oh, H. C., Yun, H. C., Oh, Y. G., Jang, G.S., hwang, S. I,, Kwon, K. M., Lee, T. O., Chung, H. T. :Imperatorin, a furanocoumarin from Angrlicae dahuricae, induces cytochrome c-dependent apoptosis in human promyelocytic leukaemia, HL-60 cells. Pharmacology and Toxicology Journal, 91(1), 40-48, 2002 https://doi.org/10.1034/j.1600-0773.2002.910107.x
  18. Wang, N. H., Yoshiazaki, K., Baba, K. : Seven new bifuranocoumarins, dahuribirin A-G, from Japanese Bai Zh. Chemical Pharmaceutical Bulletin, 49(9), 1085-1088, 2001 https://doi.org/10.1248/cpb.49.1085
  19. Jin, M. H., Jung, M. H., Lim, Y. H., Lee, S. H., Kang, S. J., Cho. W. G. : Promoting synthesis of collagen from Angelica dahurica root. Korean Journal of Pharmacognosy, 35(4), 315-319, 2004
  20. Yang, S. L., Jang, S. M., Kim, N. W. : Antioxidative activity and physiological function of the Angelica dahurica root. Journal of Food Science and Nutrition, 36(1), 20-26, 2007
  21. Kim, C. M., Kwon, Y. S., Chio, S. Y. : Antithrombotic effect of the BuOH soluble fraction of Angellica dahurica root. Korean Journal of Pharmacognosy, 26, 74-77, 1995
  22. Bolognia, J. L., Murray, M. S., Pawelek, J. M. : Hairless pigmented guinea pigs : a new model for the study of mammalian pigmentation. Pigment Cell Research, 3(3), 150-156, 1990 https://doi.org/10.1111/j.1600-0749.1990.tb00280.x
  23. Yoshida, M., Hirotsu, S., Nakahara, M., Uchiwa, H.,Tomita, Y. : Histamine is involved in ultraviolet Binduced pigmentation of guinea pig skin. Journal of Investigative Dermatology, 118(2), 255-260, 2002 https://doi.org/10.1046/j.0022-202x.2001.01668.x
  24. Choi, S. Y., Kim, S. H., Hwang, J. S., Lee, B. G., Kim, H . C., Kim, S . Y. : B enzy lamide d erivative compound attenuates the ultraviolet B-induced hyperpigmentation hyperpigmentation in the brownish guinea pig skin. Biochemical Pharmacology, 67, 707-715, 2004 https://doi.org/10.1016/j.bcp.2003.09.045
  25. Snell, R. S. : The effect of ultravioet irradiation on melanogenesis. Journal of Investigative Dermatology, 40, 127-132, 1963 https://doi.org/10.1038/jid.1963.24
  26. Park, E. Y., Lee, T. J., Kim, Y. C. : Effects of white tea water extract on skin whitening using in vivo test. Journal of Korean Society of Cosmetology, 15(2), 602-610, 2009
  27. Clarys, P., Aleqaeters, K., Lambrecht, R., Barel, A. O. :Skin color measurements: comparison between three instruments: the chromameter, the dermaspectrometer and the mexameter. Skin Research and Technology, 6(4), 230-238, 2000 https://doi.org/10.1034/j.1600-0846.2000.006004230.x
  28. Kang, S. K., Park, J. S., Kim, K. R. : A s tudy on the association of the facial skin erythema index and melanin index and the general factors, Journal of the Society of Cosmetic Scientists of Korea, 13(2), 507-513, 2007
  29. Pathak, M. A., Fitzpatrick, T. B. : Preventive treatment of sunburn, dermatoheliosis, and skin cancer with sun protective agents. Dermatology in general medicine. New York McGraw-Hill. 1689-1717, 1993
  30. Masson, P. : Carcinoids (Argentaffin-cell tumors) and nerve hyperplasia of the appendicular mucosa. American Journal. of Pathology, 4(3), 181-211, 1928
  31. Agar, N., Young, A. R. : Melanogenesis: a photoprotective response to DNA damage?. Mutation Research, 571(1), 121-132, 2005 https://doi.org/10.1016/j.mrfmmm.2004.11.016
  32. Kahn H. J., Baumil, R., Marks, A. : The value of immunohistochemical studies using antibody to S-100 protein in dermatopathology. Journal of Investigative Dermatology, 21, 38-44, 1984
  33. Megumi, T., Haratake, A., Kaminaga, H., Nakahara, M., Komiya, A., Koishikawa, H., Uchiwa, H., Kawa, Y., Mizoguchi, M. : Pigmentation in intrinsically aged skin of A1 guinea pigs. Pigment Cell Research, 17(6), 651-658, 2004 https://doi.org/10.1111/j.1600-0749.2004.00183.x

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