Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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A Prospective Comparison of Fertilizability of in vitro Matured Human Oocytes Obtained from Stimulated Cycle: Conventional Versus ICSI

과배란유도 주기에서 얻어진 체외성숙 난자의 수정능: 고식적 체외수정시술과 세포질내정자주입법의 비교

  • Jee, Byung-Chul (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital) ;
  • An, So-Jung (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital) ;
  • Moon, Jeong-Hee (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital) ;
  • Hwang, Eun-Ju (Department of Obstetrics and Gynecology, Seoul National University College of Medicine) ;
  • Suh, Chang-Suk (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital) ;
  • Kim, Seok-Hyun (Department of Obstetrics and Gynecology, Seoul National University College of Medicine) ;
  • Moon, Shin-Yong (Department of Obstetrics and Gynecology, Seoul National University College of Medicine)
  • 지병철 (분당서울대학교병원 산부인과) ;
  • 안소정 (분당서울대학교병원 산부인과) ;
  • 문정희 (분당서울대학교병원 산부인과) ;
  • 황은주 (서울대학교 의과대학 산부인과학교실) ;
  • 서창석 (분당서울대학교병원 산부인과) ;
  • 김석현 (서울대학교 의과대학 산부인과학교실) ;
  • 문신용 (서울대학교 의과대학 산부인과학교실)
  • Published : 2009.12.30
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Abstract

Objective: The aim of this study was to compare the fertilization and cleavage rates of human in vitro matured oocytes after fertilized by conventional in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). Methods: A total of 135 GV stage oocytes were obtained from 59 women who received ovarian stimulation and IVF during Jan 2007 to Oct 2008. Ovarian hyperstimulation was performed using hMG or recombinant FSH with GnRH antagonist and then ovulation triggered by recombinant hCG. The immature oocytes obtained from stimulation cycles were cultured in IVM medium up to 48 hrs; commercial medium supplemented with rFSH 75 mIU/mL, rhCG 0.5 IU/mL and rEGF 10 ng/mL. The in vitro matured oocytes were fertilized by conventional IVF (41 GV oocytes) or ICSI method (94 GV oocytes). Results: Maturation rate were 51.2% and 59.6% in conventional IVF group and ICSI group, respectively. There was no significant difference in fertilization rates between two groups; 71.4% and 80.4%, respectively. The cleavage rate was also similar in two groups. Conclusion: The presented data suggest that conventional IVF has comparable fertilization and cleavage potential compared with ICSI as the insemination method of immature human oocytes obtained from stimulated cycle.

목 적: 고식적 체외수정시술과 세포질내정자주입법을 사용하여 체외성숙 난자를 수정시키고 수정률과 배발달율을 비교하고자 하였다. 연구방법: 2007년 1월부터 2008년 8월까지 난소과자극과 체외수정시술을 받은 59명의 여성에서 135개의 배아소포 단계의 미성숙난자만을 얻어 75 mIU/mL rFSH, 0.5 IU/mL rhCG, 10 ng/mL rEGF가 포함된 체외성숙용 배양액에서 최대 48시간까지 배양하였다. 체외성숙된 난자는 고식적 체외수정방법 (n=41) 또는 세포질내정자주입법 (n=94)을 이용하여 수정시켰으며 이후 배발달율을 관찰하였다. 결 과: 고식적 체외수정군과 세포질내정자주입군에서 체외성숙률은 각각 51.2%와 59.6%였고, 수정률은 각각 71.4%와 80.4%로 두 군간에 통계적으로 유의한 차이는 없었다. 배발달율도 두 군에서 비슷하게 관찰되었다. 결 론: 난소과자극을 통해 얻은 미성숙난자는 고식적 체외수정법을 사용하더라도 세포질내정자주입법과 동등한 수정률과 배발달율을 보임을 확인하였다.

Keywords

References

  1. Chian RC. In-vitro maturation of immature oocytes for infertile women with PCOS. Reprod Biomed Online 2004; 8: 547-52 https://doi.org/10.1016/S1472-6483(10)61101-7
  2. Jee BC, Han SH, Moon JH, Suh CS, Kim SH. Influence of well-defined protein source on in vitro maturation of human oocyte: human follicular fluid versus human serum albumin. Fertil Steril 2008; 89: 348-52 https://doi.org/10.1016/j.fertnstert.2007.02.052
  3. Lim JH, Yang SH, Chian RC. New alternative to infertility treatment for women without ovarian stimulation. Reprod Biomed Online 2007; 14: 547-9 https://doi.org/10.1016/S1472-6483(10)61042-5
  4. Holzer H, Scharf E, Chian RC, Demirtas E, Buckett W, Tan SL. IVM of oocytes collected from unstimulated ovaries for oocyte donation. Fertil Steril 2007; 88: 62-7 https://doi.org/10.1016/j.fertnstert.2006.11.087
  5. Huang JY, Tulandi T, Holzer H, Tan SL, Chian RC. Combining ovarian tissue cryobanking with retrieval of immature oocytes followed by IVM and vitrification: an additional strategy of fertility preservation. Fertil Steril 2008; 89: 567-72 https://doi.org/10.1016/j.fertnstert.2007.03.090
  6. Oktay K, Demirtas E, Son WY, Lostritto K, Chian RC, Tan SL. In vitro maturation of germinal vesicle oocytes recovered after premature luteinizing hormone surge: description of a novel approach to fertility preservation. Fertil Steril 2008; 89: 228.e19-22 https://doi.org/10.1016/j.fertnstert.2007.02.028
  7. Heindryckx B, De Sutter P, Gerris J, Dhont M, Van der Elst J. Embryo development after successful somatic cell nuclear transfer to in vitro matured human germinal vesicle oocytes. Hum Reprod 2007; 22: 1982-90 https://doi.org/10.1093/humrep/dem106
  8. Jurema MW, Nogueira D. In vitro maturation of human oocytes for assisted reproduction. Fertil Steril 2006; 86: 1277-91 https://doi.org/10.1016/j.fertnstert.2006.02.126
  9. Hyun CS, Cha JH, Son WY, Yoon SH, Kim KA, Lim JH. Optimal ICSI timing after the first polar body extrusion in in-vitro matured human oocytes. Hum Reprod 2007; 22: 1991-5 https://doi.org/10.1093/humrep/dem124
  10. Hwang JL, Lin YH, Tsai YL. In vitro maturation and fertilization of immature oocytes: a comparative study of fertilization techniques. J Assist Reprod Genet 2000; 17: 39-43 https://doi.org/10.1023/A:1009450014064
  11. Soderstrom-Anttila V, Mäkinen S, Tuuri T, Suikkari AM. Favourable pregnancy results with insemination of in vitro matured oocytes from unstimulated patients. Hum Reprod 2005; 20: 1534-40 https://doi.org/10.1093/humrep/deh768
  12. Barnes FL, Kausche A, Tiglias J, Wood C, Wilton L, Trounson AO. Production of embryos from in vitro-matured primary human oocytes. Fertil Steril 1996; 65: 1151-6 https://doi.org/10.1016/S0015-0282(16)58330-7
  13. Nagy ZP, Cecile J, Liu J, Loccufier A, Devroey P, Van Steirteghem A. Pregnancy and birth after intracytoplasmic sperm injection of in vitro matured germinal-vesicle stage oocytes: case report. Fertil Steril 1996; 65: 1047-50
  14. De Vos A, Van Steirteghem A. Zona hardening, zona drilling and assisted hatching: new achievements in assisted reproduction. Cells Tissues Organs 2000; 166: 220-7 https://doi.org/10.1159/000016734
  15. Cavilla JL, Kennedy CR, Byskov AG, Hartshorne GM. Human immature oocytes grow during culture for IVM. Hum Reprod 2008; 23: 37-45